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CC BY-NC-ND 4.0 · Rev Bras Ortop (Sao Paulo) 2022; 57(02): 218-222
DOI: 10.1055/s-0041-1729592
Artigo Original
Trauma

Hematological Changes after Caprine Demineralized Bone Matrix Implantation in Ulnar Critical Defect of Rabbit Model[*]

Artikel in mehreren Sprachen: português | English
Olawale Alimi Alimi 
1   Departamento de Cirurgia e Radiologia Veterinária, University of Ilorin, Ilorin, Nigéria
2   Departamento de Cirurgia e Radiologia Veterinária, Usmanu Danfodiyo University, Sokoto, Nigéria
,
Adamu Abdul Abubakar 
2   Departamento de Cirurgia e Radiologia Veterinária, Usmanu Danfodiyo University, Sokoto, Nigéria
,
Abubakar Sadiq Yakubu 
2   Departamento de Cirurgia e Radiologia Veterinária, Usmanu Danfodiyo University, Sokoto, Nigéria
,
Sani Abdullahi Shehu 
3   Departamento de Anatomia Veterinária, Usmanu Danfodiyo Universi ty, Sokoto, Nigéria
,
Salman Zubairu Abdulkadir 
1   Departamento de Cirurgia e Radiologia Veterinária, University of Ilorin, Ilorin, Nigéria
2   Departamento de Cirurgia e Radiologia Veterinária, Usmanu Danfodiyo University, Sokoto, Nigéria
,
Ekaete Ime Oviawe 
2   Departamento de Cirurgia e Radiologia Veterinária, Usmanu Danfodiyo University, Sokoto, Nigéria
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Abstract

Objective Several animal models have been used in fracture healing and bone graft studies, but hematological responses are seldom reported. Therefore, the present study reported the hematological changes observed in rabbits that underwent xenografting of caprine demineralized bone matrix (CDBM).

Method Twenty-four (24) male rabbits (2.5 ± 0.5kg) were acquired for the purpose of this study and were randomly assigned to three groups: autologous bone graft (ABG), unfilled (NC), and caprine demineralized bone matrix (CDBM). Blood samples were collected through cardiac puncture under xylazine-ketamine anesthesia on day 0 (baseline), and on days 28 and 56 postsurgery and were analyzed manually within 2 hours of collection. Statistical analysis was performed using a two-way analysis of variance (ANOVA) with repeated measures, and a p-value < 0.05 was considered significant.

Result There was an overall significant difference in the values of total white blood cell count (p = 0.0043), neutrophil count (p < 0.0001), monocyte count (p = 0.0184), red blood cell count (p = 0.003), hemoglobin concentration (p < 0.0001) and packed cell volume (p < 0.0001) across the days and the treatment groups. There was, however, no overall significant difference in lymphocyte count (p = 0.4923), basophil count (p = 0.4183), and eosinophil count (0.4806) within days.

Conclusion Response to CDBM grafting in rabbits could, therefore, be said to be characterized by marked leukocytosis with neutrophilia, lymphocytosis, and monocytosis by day 28 of postgrafting. This could form the basis with which hematology can be used to monitor body response of bone graft animal models.

Keywords

bone grafting - bone xenograft - experimental animal model - fracture healing - hematology

* Work developed at the Department of Veterinary Surgery and Radiology at Usmanu Danfodiyo University, Sokoto, Nigeria.




Publikationsverlauf

Eingereicht: 29. August 2020

Angenommen: 08. Januar 2021

Artikel online veröffentlicht:
13. August 2021

© 2021. Sociedade Brasileira de Ortopedia e Traumatologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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