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CC BY-NC-ND 4.0 · Rev Bras Ortop (Sao Paulo) 2022; 57(02): 218-222
DOI: 10.1055/s-0041-1729592
Artigo Original
Trauma

Hematological Changes after Caprine Demineralized Bone Matrix Implantation in Ulnar Critical Defect of Rabbit Model[*]

Article in several languages: português | English
Olawale Alimi Alimi 
1   Departamento de Cirurgia e Radiologia Veterinária, University of Ilorin, Ilorin, Nigéria
2   Departamento de Cirurgia e Radiologia Veterinária, Usmanu Danfodiyo University, Sokoto, Nigéria
,
Adamu Abdul Abubakar 
2   Departamento de Cirurgia e Radiologia Veterinária, Usmanu Danfodiyo University, Sokoto, Nigéria
,
Abubakar Sadiq Yakubu 
2   Departamento de Cirurgia e Radiologia Veterinária, Usmanu Danfodiyo University, Sokoto, Nigéria
,
Sani Abdullahi Shehu 
3   Departamento de Anatomia Veterinária, Usmanu Danfodiyo Universi ty, Sokoto, Nigéria
,
Salman Zubairu Abdulkadir 
1   Departamento de Cirurgia e Radiologia Veterinária, University of Ilorin, Ilorin, Nigéria
2   Departamento de Cirurgia e Radiologia Veterinária, Usmanu Danfodiyo University, Sokoto, Nigéria
,
Ekaete Ime Oviawe 
2   Departamento de Cirurgia e Radiologia Veterinária, Usmanu Danfodiyo University, Sokoto, Nigéria
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Abstract

Objective Several animal models have been used in fracture healing and bone graft studies, but hematological responses are seldom reported. Therefore, the present study reported the hematological changes observed in rabbits that underwent xenografting of caprine demineralized bone matrix (CDBM).

Method Twenty-four (24) male rabbits (2.5 ± 0.5kg) were acquired for the purpose of this study and were randomly assigned to three groups: autologous bone graft (ABG), unfilled (NC), and caprine demineralized bone matrix (CDBM). Blood samples were collected through cardiac puncture under xylazine-ketamine anesthesia on day 0 (baseline), and on days 28 and 56 postsurgery and were analyzed manually within 2 hours of collection. Statistical analysis was performed using a two-way analysis of variance (ANOVA) with repeated measures, and a p-value < 0.05 was considered significant.

Result There was an overall significant difference in the values of total white blood cell count (p = 0.0043), neutrophil count (p < 0.0001), monocyte count (p = 0.0184), red blood cell count (p = 0.003), hemoglobin concentration (p < 0.0001) and packed cell volume (p < 0.0001) across the days and the treatment groups. There was, however, no overall significant difference in lymphocyte count (p = 0.4923), basophil count (p = 0.4183), and eosinophil count (0.4806) within days.

Conclusion Response to CDBM grafting in rabbits could, therefore, be said to be characterized by marked leukocytosis with neutrophilia, lymphocytosis, and monocytosis by day 28 of postgrafting. This could form the basis with which hematology can be used to monitor body response of bone graft animal models.

Keywords

bone grafting - bone xenograft - experimental animal model - fracture healing - hematology

* Work developed at the Department of Veterinary Surgery and Radiology at Usmanu Danfodiyo University, Sokoto, Nigeria.




Publication History

Received: 29 August 2020

Accepted: 08 January 2021

Article published online:
13 August 2021

© 2021. Sociedade Brasileira de Ortopedia e Traumatologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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  • Referências

  • 1 Alimi OA, Abdulwahab WF, Amid SA. et al. Hematological prediction study of peritonitis following laparotomy in goats. J Vet Med Sci 2020; 82 (05) 531-535
    CrossrefPubMedSearch in Google Scholar
  • 2 Tambuwal FM, Agaie BM, Bangana A. Haematological and Biochemical Values of Apparently Healthy Red Sokoto Goats. In: Proceeding of 27th Annual Conference. FUTA, Akure, Nigeria: Nigerian Society of Animal production (NSAP) 2002: 50-53
    PubMedSearch in Google Scholar
  • 3 Akinrinmade JF, Akinrinde AS. Hematological and serum biochemical indices of West African dwarf goats with foreign body rumen impaction. Niger J Physiol Sci 2012; 27 (01) 83-87
    PubMedSearch in Google Scholar
  • 4 Etim NN, Williams ME, Akpabio U, Offiong EEA. Haematological Parameters and Factors Affecting Their Values. Agric Sci 2014; 2 (01) 37-47
    Search in Google Scholar
  • 5 Aderemi FA. Effects of replacement of wheat bran with cassava root sieviatesupplemented or unsupplemented with enzyme on the haematology and serum biochemistry of pullet chicks. Trop J Anim Sci 2004; 7 (01) 147-153
    Search in Google Scholar
  • 6 Doyle D. William Hewson (1739-74): the father of haematology. Br J Haematol 2006; 133 (04) 375-381
    CrossrefPubMedSearch in Google Scholar
  • 7 Chineke C, Ologun A, Ikeobi CO. Haematological Parameters in Rabbit Breeds and Crosses in Humid Tropics. Pak J Biol Sci 2006; 9 (11) 2102-2106
    CrossrefSearch in Google Scholar
  • 8 Moore DM, Zimmerman K, Smith SA. Hematological Assessment in Pet Rabbits: Blood Sample Collection and Blood Cell Identification. Clin Lab Med 2015; 35 (03) 617-627
    CrossrefPubMedSearch in Google Scholar
  • 9 Kumar D, Bhargava MK, Singh R. et al. Haematological Changes during Fracture Healing in Goats. IOSR J Agric Vet Sci 2016; 9 (09) 1-3
    CrossrefSearch in Google Scholar
  • 10 Arpağ OF, Damlar I, Altan A, Tatli U, Günay A. To what extent does hyaluronic acid affect healing of xenografts? A histomorphometric study in a rabbit model. J Appl Oral Sci 2018; 26 (00) e20170004
    CrossrefPubMedSearch in Google Scholar
  • 11 Monazzah S, Oryan A, Bigham-Sadegh A, Meimandi-Parizi A. Application of bovine bone versus bovine DBM graft on bone healing of radial defect in rat. Comp Clin Pathol 2017; 26 (06) 1293-1298
    CrossrefSearch in Google Scholar
  • 12 Bigham-Sadegh A, Oryan A. Selection of animal models for pre-clinical strategies in evaluating the fracture healing, bone graft substitutes and bone tissue regeneration and engineering. Connect Tissue Res 2015; 56 (03) 175-194
    CrossrefPubMedSearch in Google Scholar
  • 13 Ajai S, Sabir A, Mahdi AA, Srivastava RN. Evaluation of Serum Alkaline Phosphatase as a Biomarker of Healing Process Progression of Simple Diaphyseal Fractures in Adult Patients. Int Res J Biol Sci Int Res J Biol Sci 2013; 2 (02) 2278-3202
    Search in Google Scholar
  • 14 Bigham-Sadegh A, Mirshokraei P, Karimi I, Oryan A, Aparviz A, Shafiei-Sarvestani Z. Effects of adipose tissue stem cell concurrent with greater omentum on experimental long-bone healing in dog. Connect Tissue Res 2012; 53 (04) 334-342
    CrossrefPubMedSearch in Google Scholar
  • 15 Korkmaz M, Oztürk H, Bulut O, Unsaldi T, Kaloğlu C. [The effect of definitive continuous distraction employed with the Ilizarov type external fixation system on fracture healing: an experimental rabbit model]. Acta Orthop Traumatol Turc 2005; 39 (03) 247-257
    PubMedSearch in Google Scholar
  • 16 Washington IM, Van Hoosier G. Clinical Biochemistry and Hematology. The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents. 2012: 57-116
    Search in Google Scholar
  • 17 Toth C, Klarik Z, Kiss F, Toth E, Hargitai Z, Nemeth N. Early postoperative changes in hematological, erythrocyte aggregation and blood coagulation parameters after unilateral implantation of polytetrafluoroethylene vascular graft in the femoral artery of beagle dogs. Acta Cir Bras 2014; 29 (05) 320-327
    CrossrefPubMedSearch in Google Scholar
  • 18 Grover RK, Sobti VK. Clinical, haematological and radiological evaluation of fragmented autogenous cortical bone grafting of radius in dogs. Zentralbl Veterinarmed A 1998; 45 (05) 303-308
    CrossrefPubMedSearch in Google Scholar
  • 19 Arens D, Wilke M, Calabro L. et al. A rabbit humerus model of plating and nailing osteosynthesis with and without Staphylococcus aureus osteomyelitis. Eur Cell Mater 2015; 30: 148-161 , discussion 161–162
    CrossrefPubMedSearch in Google Scholar
  • 20 Melillo A. Rabbit Clinical Pathology. J Exot Pet Med 2007; 16 (03) 135-145
    CrossrefPubMedSearch in Google Scholar
  • 21 Guizzardi S, Di Silvestre M, Scandroglio R, Ruggeri A, Savini R. Implants of heterologous demineralized bone matrix for induction of posterior spinal fusion in rats. Spine 1992; 17 (06) 701-707
    CrossrefPubMedSearch in Google Scholar
  • 22 Bigham AS, Shadkhast M, Bigham Sadegh A, Shafiei Z, Lakzian A, Khalegi MR. Evaluation of osteoinduction properties of the demineralized bovine foetal growth plate powder as a new xenogenic biomaterial in rat. Res Vet Sci 2011; 91 (02) 306-310
    CrossrefPubMedSearch in Google Scholar