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CC BY-NC-ND 4.0 · Eur J Dent 2021; 15(04): 702-706
DOI: 10.1055/s-0041-1730041
Original Article

Association between Interleukin-1β Gene Polymorphism and Chronic Periodontitis

Muhammad Mansoor Majeed
1   Department of Oral Biology, Altamash Institute of Dental Medicine, Karachi, Pakistan
,
Imtiaz Ahmed
2   Department of Orthodontics, Dow University of Health Sciences, Karachi, Pakistan
,
Talat Roome
3   Section of Molecular Pathology, Department of Pathology, Dow Diagnostic Reference and Research Laboratory, Dow University of Health Sciences, Karachi, Pakistan
,
Tehseen Fatima
4   Dow College of Biotechnology, Dow University of Health Sciences, Karachi, Pakistan
,
Rafat Amin
5   Dow College of Biotechnology, Dow University of Health Sciences, Karachi, Pakistan
› Author Affiliations Funding None.
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Abstract

Objectives Periodontitis is a pathological condition of the oral cavity, originating from multiple factors, including microbial, environmental and genetic factors. The vulnerability to several pathologies has been studied with the relationship to genetic polymorphisms, and one of the most prominent is the single nucleotide polymorphisms throughout the genome. The study aimed to find out the association of single nucleotide polymorphism (SNP) of interleukin-1β +3954 gene with chronic periodontitis (CP) in Pakistan

Materials and Methods This case–control study was conducted at Dow University of Health Sciences. DNA was extracted from the blood and amplified by using conventional polymerase chain reaction of respective genes followed by sequencing. Mann–Whitney test accessed the difference of clinical parameters between cases and controls, and Fisher’s exact test was applied to access the association of alleles between subjects. Data entered and analyzed using SPSS 21.

Results Significant differences were observed in clinical parameters in cases and controls (p < 0.001). In the IL-1β +3954 gene, T alleles were significantly higher in cases as compared with controls (p < 0.001). Genotype CC was significantly dominant in the controls and genotype CT and TT in patients (Chi-square = 19.83, p < 0.001).

Conclusion Within the study’s limits, IL-1β +3954 gene polymorphism is associated with periodontitis and is expected to be among the several causes of respective pathology in Pakistan’s population.

Keywords

Chronic periodontitis - Interleukin-1β gene - Pakistan - single nucleotide - polymorphism


Publication History

Article published online:
24 July 2021

© 2021. European Journal of Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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  • References

  • 1 Kassebaum NJ, Bernabé E, Dahiya M, Bhandari B, Murray CJ, Marcenes W. Global burden of severe periodontitis in 1990-2010: a systematic review and meta-regression. J Dent Res 2014; 93 (11) 1045-1053
    CrossrefPubMedGoogle Scholar
  • 2 Armitage GC, Wu Y, Wang HY, Sorrell J, di Giovine FS, Duff GW. Low prevalence of a periodontitis-associated interleukin-1 composite genotype in individuals of Chinese heritage. J Periodontol 2000; 71 (02) 164-171
    CrossrefPubMedGoogle Scholar
  • 3 Greenwood D, Slack RC, Barer MR, Irving WL. Medical Microbiology: A Guide to Microbial Infections: Pathogenesis, Immunity, Laboratory Diagnosis, and Control. With student consult online access: Elsevier Health Sciences 2012
  • 4 Arora S, Ramachandra SS, Abdullah F, Gundavarapu KC. Interleukin 1β (+ 3954;− 511) genotype polymorphism and its association with severe chronic generalized periodontitis in the Malaysian Population. Contemp Clin Dent 2017; 8 (01) 102-105
    CrossrefPubMedGoogle Scholar
  • 5 Heidari Z, Moudi B, Mahmoudzadeh-Sagheb H. Immunomodulatory factors gene polymorphisms in chronic periodontitis: an overview. BMC Oral Health 2019; 19 (01) 29
    CrossrefPubMedGoogle Scholar
  • 6 Shazam H, Shaikh F, Hussain Z, Majeed MM, Khan S, Khurshid Z. Evaluation of osteocalcin levels in saliva of periodontitis patients and their correlation with the disease severity: a cross-sectional study. Eur J Dent 2020; 14 (03) 352-359
    Article in Thieme ConnectPubMedGoogle Scholar
  • 7 Hamza SA, Wahid A, Afzal N. et al Effect of sodium bicarbonate mouth wash on salivary ph and interleukin-1β levels among smokers. Eur J Dent 2020; 14 (02) 260-267
    Article in Thieme ConnectPubMedGoogle Scholar
  • 8 Sharma S, Saimbi CS, Koirala B, Shukla R. Effect of various mouthwashes on the levels of interleukin-2 and interferon-γ in chronic gingivitis. J Clin Pediatr Dent 2008; 32 (02) 111-114
    CrossrefPubMedGoogle Scholar
  • 9 Nakamura I, Jimi E. Regulation of osteoclast differentiation and function by interleukin-1. Vitam Horm 2006; 74: 357-370
    CrossrefPubMedGoogle Scholar
  • 10 Ng PYB, Donley M, Hausmann E, Hutson AD, Rossomando EF, Scannapieco FA. Candidate salivary biomarkers associated with alveolar bone loss: cross-sectional and in vitro studies. FEMS Immunol Med Microbiol 2007; 49 (02) 252-260
    CrossrefPubMedGoogle Scholar
  • 11 He K, Jian F, He T, Tang H, Huang B, Wei N. Analysis of the association of TNF-α, IL-1A, and IL-1B polymorphisms with peri-implantitis in a Chinese non-smoking population. Clin Oral Investig 2020; 24 (02) 693-699
    CrossrefPubMedGoogle Scholar
  • 12 Cheng R, Wu Z, Li M, Shao M, Hu T. Interleukin-1β is a potential therapeutic target for periodontitis: a narrative review. Int J Oral Sci 2020; 12 (01) 1-9
    CrossrefPubMedGoogle Scholar
  • 13 Huang W, He B-Y, Shao J, Jia X-W, Yuan Y-D. Interleukin-1β rs1143627 polymorphism with susceptibility to periodontal disease. Oncotarget 2017; 8 (19) 31406-31414
    CrossrefPubMedGoogle Scholar
  • 14 Lobene RR, Weatherford T, Ross NM, Lamm RA, Menaker L. A modified gingival index for use in clinical trials. Clin Prev Dent 1986; 8 (01) 3-6
    Google Scholar
  • 15 Loe H, Silness J. Periodontal disease in pregnancy. I. Prevalence and severity. Acta Odontol Scand 1963; 21: 533-551
    CrossrefPubMedGoogle Scholar
  • 16 Ramfjord SP. Indices for prevalence and incidence of periodontal disease. J Periodontol 1959; 30 (01) 51-59
    CrossrefGoogle Scholar
  • 17 Bagnall RD, Molloy LK, Kalman JM, Semsarian C. Exome sequencing identifies a mutation in the ACTN2 gene in a family with idiopathic ventricular fibrillation, left ventricular noncompaction, and sudden death. BMC Med Genet 2014; 15 (01) 99
    CrossrefPubMedGoogle Scholar
  • 18 da Silva FRP, Vasconcelos ACCG, de Carvalho França LF, Di Lenardo D, Nascimento HMS, Vasconcelos DFP. Association between the rs1143634 polymorphism in interleukin-1B and chronic periodontitis: Results from a meta-analysis composed by 54 case/control studies. Gene 2018; 668: 97-106
    CrossrefPubMedGoogle Scholar
  • 19 Smith AJ, Keen LJ, Billingham MJ. et al Extended haplotypes and linkage disequilibrium in the IL1R1-IL1A-IL1B-IL1RN gene cluster: association with knee osteoarthritis. Genes Immun 2004; 5 (06) 451-460
    CrossrefPubMedGoogle Scholar
  • 20 Domínguez-Pérez RA, Loyola-Rodriguez JP, Abud-Mendoza C, Alpuche-Solis AG, Ayala-Herrera JL, Martínez-Martínez RE. Association of cytokines polymorphisms with chronic peridontitis and rheumatoid arthritis in a Mexican population. Acta Odontol Scand 2017; 75 (04) 243-248
    CrossrefPubMedGoogle Scholar
  • 21 He Z, Sun Y, Wu J. et al Evaluation of genetic variants in IL-1B and its interaction with the predisposition of osteoporosis in the northwestern Chinese Han population. J Gene Med 2020; 22 (10) e3214
    PubMedGoogle Scholar
  • 22 Majumder P, Panda SK, Ghosh S, Dey SK. Interleukin gene polymorphisms in chronic periodontitis: a case-control study in the Indian population. Arch Oral Biol 2019; 101: 156-164
    CrossrefPubMedGoogle Scholar
  • 23 Masamatti SS, Kumar A, Baron TKA, Mehta DS, Bhat K. Evaluation of interleukin -1B (+3954) gene polymorphism in patients with chronic and aggressive periodontitis: a genetic association study. Contemp Clin Dent 2012; 3 (02) 144-149
    CrossrefPubMedGoogle Scholar
  • 24 Moreira PR, de Sá AR, Xavier GM. et al A functional interleukin-1 β gene polymorphism is associated with chronic periodontitis in a sample of Brazilian individuals. J Periodontal Res 2005; 40 (04) 306-311
    CrossrefPubMedGoogle Scholar
  • 25 Ma L, Chu W-M, Zhu J, Wu Y-N, Wang Z-L. Interleukin-1β (3953/4) C→T polymorphism increases the risk of chronic periodontitis in Asians: evidence from a meta-analysis of 20 case-control studies. Arch Med Sci 2015; 11 (02) 267-273
    CrossrefPubMedGoogle Scholar
  • 26 Mazurek-Mochol M, Dembowska E, Malinowski D, Safranow K, Pawlik A. IL-1β rs1143634 and rs16944 polymorphisms in patients with periodontal disease. Arch Oral Biol 2019; 98: 47-51
    CrossrefPubMedGoogle Scholar
  • 27 Das A, Kundu R, Mondal D, Pal AK, Bagchi S. Evaluation of genetic polymorphism of interleukin-1β+ 3954 (rs1143634) in chronic & aggressive periodontitis in Bengali population of West Bengal, India. Int J Adv Sci Res 2020; 5 (04) 61-66
    Google Scholar