Association between Interleukin-1β Gene Polymorphism and Chronic Periodontitis

Muhammad Mansoor Majeed; Imtiaz Ahmed; Talat Roome; Tehseen Fatima; Rafat Amin

doi:10.1055/s-0041-1730041

European Journal of Dentistry, Table of Contents

CC BY-NC-ND 4.0 · Eur J Dent 2021; 15(04): 702-706
DOI: 10.1055/s-0041-1730041

Original Article

Association between Interleukin-1β Gene Polymorphism and Chronic Periodontitis

Muhammad Mansoor Majeed

¹Department of Oral Biology, Altamash Institute of Dental Medicine, Karachi, Pakistan

,

Imtiaz Ahmed

²Department of Orthodontics, Dow University of Health Sciences, Karachi, Pakistan

,

Talat Roome

³Section of Molecular Pathology, Department of Pathology, Dow Diagnostic Reference and Research Laboratory, Dow University of Health Sciences, Karachi, Pakistan

,

Tehseen Fatima

⁴Dow College of Biotechnology, Dow University of Health Sciences, Karachi, Pakistan

,

Rafat Amin

⁵Dow College of Biotechnology, Dow University of Health Sciences, Karachi, Pakistan

› Author Affiliations

Abstract

Objectives Periodontitis is a pathological condition of the oral cavity, originating from multiple factors, including microbial, environmental and genetic factors. The vulnerability to several pathologies has been studied with the relationship to genetic polymorphisms, and one of the most prominent is the single nucleotide polymorphisms throughout the genome. The study aimed to find out the association of single nucleotide polymorphism (SNP) of interleukin-1β +3954 gene with chronic periodontitis (CP) in Pakistan

Materials and Methods This case–control study was conducted at Dow University of Health Sciences. DNA was extracted from the blood and amplified by using conventional polymerase chain reaction of respective genes followed by sequencing. Mann–Whitney test accessed the difference of clinical parameters between cases and controls, and Fisher’s exact test was applied to access the association of alleles between subjects. Data entered and analyzed using SPSS 21.

Results Significant differences were observed in clinical parameters in cases and controls (p < 0.001). In the IL-1β +3954 gene, T alleles were significantly higher in cases as compared with controls (p < 0.001). Genotype CC was significantly dominant in the controls and genotype CT and TT in patients (Chi-square = 19.83, p < 0.001).

Conclusion Within the study’s limits, IL-1β +3954 gene polymorphism is associated with periodontitis and is expected to be among the several causes of respective pathology in Pakistan’s population.

Keywords

Chronic periodontitis - Interleukin-1β gene - Pakistan - single nucleotide - polymorphism

Full Text

References

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