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CC BY-NC-ND 4.0 · Indian J Plast Surg 2021; 54(03): 314-320
DOI: 10.1055/s-0041-1734573
Original Article

Effects of Caffeine Consumption on Autologous Full-Thickness Skin Graft Healing in an Animal Model

Tommy Supit 
1   Department of Surgery, Faculty of Medicine Diponegoro University, Dr. Kariadi General Hospital, Semarang, Jawa Tengah, Indonesia
2   Department of Biomedical Science, Faculty of Medicine Diponegoro University, Semarang, Jawa Tengah, Indonesia
,
Neni Susilaningsih
2   Department of Biomedical Science, Faculty of Medicine Diponegoro University, Semarang, Jawa Tengah, Indonesia
3   Department of Histology, Faculty of Medicine Diponegoro University, Semarang, Jawa Tengah, Indonesia
,
Awal Prasetyo
2   Department of Biomedical Science, Faculty of Medicine Diponegoro University, Semarang, Jawa Tengah, Indonesia
4   Department of Anatomical Pathology, Faculty of Medicine Diponegoro University, Dr. Kariadi General Hospital, Semarang, Jawa Tengah, Indonesia
,
Najatullah › Author Affiliations Financial Disclosure None.
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Abstract

Background There exists contradictory evidence that states both the beneficial and deleterious effects of caffeine on wound healing. The general population might unknowingly consume caffeine that negatively affects wound healing. The main objective of this study is to investigate the effect of daily caffeine consumption on wound healing, specifically full-thickness skin graft (FTSG).

Methods Forty Sprague–Dawley rats were randomized into four groups of equal size: control-dose (CD), low-dose (LD), medium-dose (MD), and high-dose (HD) caffeine groups. After autologous FTSG, all subjects in the intervention group were given daily pure caffeine gavage. The FTSG was explanted 7 days posttransplant. The graft viability, secondary contraction, and adherence were evaluated macroscopically, while fibroblast and collagen deposition was analyzed microscopically with hematoxylin eosin stain.

Results The least graft viability (72.8 ± 20.7%, clinical wound assessment scale [CWAS] 2.4), highest secondary contraction (11.4 ± 10.5%), and fibroblast count (331.8 ± 88.6 cells/5 high power fields) were observed in the MD group. More collagen synthesis was observed in subjects who consumed caffeine. The level of secondary contraction, fibroblast count as well as graft viability and collagen synthesis were positively correlated.

Conclusions Daily consumption of caffeine impairs graft viability when given in medium dose and increases collagen synthesis, irrespective of dosage. This study was in experimental rats; the results are not directly translatable to humans.

Keywords

caffeine - graft - autologous - full-thickness skin graft - wound - healing


Publication History

Article published online:
16 September 2021

© 2021. Association of Plastic Surgeons of India. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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  • References

  • 1 Ogawa R, Hyakusoku H, Ono S. Useful tips for successful skin grafting. J Nippon Med Sch 2007; 74 (06) 386-392
    CrossrefPubMedGoogle Scholar
  • 2 Cereda E, Neyens JCL, Caccialanza R, Rondanelli M, Schols JMGA. Efficacy of a disease-specific nutritional support for pressure ulcer healing: A systematic review and meta-analysis. J Nutr Health Aging 2017; 21 (06) 655-661
    CrossrefPubMedGoogle Scholar
  • 3 Poole R, Kennedy OJ, Roderick P, Fallowfield JA, Hayes PC, Parkes J. Coffee consumption and health: umbrella review of meta-analyses of multiple health outcomes. BMJ 2017; 359: j5024
    CrossrefPubMedGoogle Scholar
  • 4 Almeida AA, Farah A, Silva DAM, Nunan EA, Glória MBA. Antibacterial activity of coffee extracts and selected coffee chemical compounds against enterobacteria. J Agric Food Chem 2006; 54 (23) 8738-8743
    CrossrefPubMedGoogle Scholar
  • 5 Ojeh N, Stojadinovic O, Pastar I, Sawaya A, Yin N, Tomic-Canic M. The effects of caffeine on wound healing. Int Wound J 2016; 13 (05) 605-613
    CrossrefPubMedGoogle Scholar
  • 6 Feoktistov I, Biaggioni I, Cronstein BN. Adenosine receptors in wound healing, fibrosis and angiogenesis. Handb Exp Pharmacol 2009; ( (193) 383-397
    CrossrefPubMedGoogle Scholar
  • 7 Horrigan LA, Kelly JP, Connor TJ. Immunomodulatory effects of caffeine: friend or foe?. Pharmacol Ther 2006; 111 (03) 877-892
    CrossrefPubMedGoogle Scholar
  • 8 Spriet LL. Exercise and sport performance with low doses of caffeine. Sports Med 2014; 44 (Suppl. 02) S175-S184
    CrossrefPubMedGoogle Scholar
  • 9 Nair AB, Jacob S. A simple practice guide for dose conversion between animals and human. J Basic Clin Pharm 2016; 7 (02) 27-31
    CrossrefPubMedGoogle Scholar
  • 10 Branski LK, Mittermayr R, Herndon DN. et al A porcine model of full-thickness burn, excision and skin autografting. Burns 2008; 34 (08) 1119-1127
    CrossrefPubMedGoogle Scholar
  • 11 Šmahel J. Biology of the stage of plasmatic imbibition. Br J Plast Surg 1971; 24 (02) 140-143
    CrossrefPubMedGoogle Scholar
  • 12 Takabayashi Y, Ishihara M, Kuwabara M. et al Improved survival of full-thickness skin graft with low-molecular weight heparin-protamine micro/nanoparticles including platelet-rich plasma. Ann Plast Surg 2017; 78 (05) 562-568
    CrossrefPubMedGoogle Scholar
  • 13 Zhang F, Oswald TM, Lin L, Wang S, Lin S, Lineaweaver WC. Improvement of full-thickness skin graft survival by application of vascular endothelial growth factor in rats. Ann Plast Surg 2008; 60 (05) 589-593
    CrossrefPubMedGoogle Scholar
  • 14 Kawamata S, Ozawa J, Hashimoto M, Kurose T, Shinohara H. Structure of the rat subcutaneous connective tissue in relation to its sliding mechanism. Arch Histol Cytol 2003; 66 (03) 273-279
    CrossrefPubMedGoogle Scholar
  • 15 Naldaiz-Gastesi N, Bahri OA, López de Munain A, McCullagh KJA, Izeta A. The panniculus carnosus muscle: an evolutionary enigma at the intersection of distinct research fields. J Anat 2018; 233 (03) 275-288
    CrossrefPubMedGoogle Scholar
  • 16 Tavis MJ, Thornton JW, Harney JH, Danet RT, Woodroof EA, Bartlett RH. Mechanism of skin graft adherence: collagen, elastin, and fibrin interactions. Surg Forum 1977; 28: 522-524
    PubMedGoogle Scholar
  • 17 Arauz J, Zarco N, Segovia J, Shibayama M, Tsutsumi V, Muriel P. Caffeine prevents experimental liver fibrosis by blocking the expression of TGF-β. Eur J Gastroenterol Hepatol 2014; 26 (02) 164-173
    CrossrefPubMedGoogle Scholar
  • 18 Tatler AL, Barnes J, Habgood A, Goodwin A, McAnulty RJ, Jenkins G. Caffeine inhibits TGFβ activation in epithelial cells, interrupts fibroblast responses to TGFβ, and reduces established fibrosis in ex vivo precision-cut lung slices. Thorax 2016; 71 (06) 565-567
    CrossrefPubMedGoogle Scholar
  • 19 Donejko M, Przylipiak A, Rysiak E, Głuszuk K, Surażyński A. Influence of caffeine and hyaluronic acid on collagen biosynthesis in human skin fibroblasts. Drug Des Devel Ther 2014; 8: 1923-1928
    PubMedGoogle Scholar
  • 20 Burdan F. Pharmacology of Caffeine. In: Coffee in Health and Disease Prevention. Elsevier; 2015: 823-829
    CrossrefGoogle Scholar
  • 21 Grieb G, Steffens G, Pallua N, Bernhagen J, Bucala R. Circulating fibrocytes-biology and mechanisms in wound healing and scar formation. Int Rev Cell Mol Biol 2011; 291: 1-19
    CrossrefPubMedGoogle Scholar
  • 22 Tuncali D, Yavuz N, Cigsar B, Gokrem S, Aslan G. Effect of full-thickness skin graft initial dimension on secondary wound contraction: experimental study in rats. Dermatol Surg 2005; 31 (05) 542-545
    CrossrefPubMedGoogle Scholar
  • 23 Kamran A, Javad FM, Sahram F, Jaber MS. A comparison of survival and secondary contraction in expanded versus conventional full-thickness skin grafts: an experimental study in rats. Eplasty 2012; 12: e20
    PubMedGoogle Scholar