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CC BY 4.0 · Eur J Dent 2022; 16(03): 543-548
DOI: 10.1055/s-0041-1735794
Original Article

Validation of RNA Aptamer Probes to Image Candida albicans in Paraffin-Embedded Sections of Wistar Rat Tongue

Boy M. Bachtiar
1   Department of Oral Biology and Oral Science Research Center, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
,
Chatchawan Srisawat
2   Department of Biochemistry and NANOTEC-Mahidol, University Center of Excellence in Nanotechnology for Cancer Diagnosis and Treatment, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok Noi, Bangkok, Thailand
,
Retno Pudji Rahayu
3   Department of Oral and Maxillofacial Pathology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Retno D. Soedjodono
4   Department of Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia
,
Silvia Arin Prabandari
5   Primate Research Center, IPB University, Bogor, Indonesia
,
Endang W. Bachtiar
1   Department of Oral Biology and Oral Science Research Center, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
› Institutsangaben
Funding This work was supported by a research grant from Kemenristek/BRIN 2020 to B.M.B.
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Abstract

Objective This study aimed to validate the use of Ca-apt-1, an RNA aptamer, that we generated previously as a probe for immunostaining of Candida albicans in rat tongue paraffin-fixed tissue sections

Material and Methods The performance of Ca-apt-1 as a detector molecule was compared with that of anti-C. albicans polyclonal antibody (PcAb), which was used as a positive control. Immunostaining images were visualized by light microscopy and were analyzed by using ImageJ software.

Results Microscopic results demonstrated that Ca-apt-1 specifically recognized and immunostained C. albicans cells of rat tongue candidiasis, with a specificity comparable to that of PcAb. ImageJ analysis showed that the area (pixels) detected by Ca-apt-1 was wider than that detected by the antibody. This indicates that the binding affinity of Ca-apt-1 toward C. albicans was better than that of PcAb on paraffin-embedded tissues.

Conclusion This study demonstrates that Ca-apt-1 can be used as a probe for immunostaining of fixed tissue sections for oral candidiasis diagnosis.

Keywords

aptamer probe - Candida albicans - immunostaining - paraffin-embedded tissue staining - oral candidiasis


Publikationsverlauf

Artikel online veröffentlicht:
25. Oktober 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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