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CC BY 4.0 · Eur J Dent 2022; 16(02): 367-372
DOI: 10.1055/s-0041-1735907
Original Article

Association of HPV and EBV in Oral Verrucous Squamous Cell Carcinoma and Oral Verrucous Hyperplasia

Eakapong Tamboon
1   Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Phetmany Sihavong
2   Dental Basic Science Department, Faculty of Dentistry, University of Health Science, Lao P.D.R, Thailand
3   Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Nakarin Kitkumthorn
1   Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Dusit Bumalee
3   Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Tawepong Arayapisit
4   Department of Anatomy, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Puangwan Lapthanasupkul
3   Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
› Author Affiliations Funding This research was partially supported by International Dental Collaboration of The Mekong River Region (IDCMR) scholarship, Faculty of Dentistry, Mahidol University.
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Abstract

Objective Oral verrucous squamous cell carcinoma or oral verrucous carcinoma (OVC) is a rare verrucous variant of oral squamous cell carcinoma (OSCC), which accounts for 2 to 12% of all oral carcinomas. Oral verrucous hyperplasia (OVH) is clinically similar to OVC and has been proposed to be a precursor lesion of OVC. Etiopathogenesis of both lesions is still inconspicuous. Oncogenic viruses such as human papillomavirus (HPV) and Epstein–Barr virus (EBV) have been reported to be associated with some cases of OSCC, and we hypothesized that it may act as a causative agent of these verrucous lesions. This study aimed to investigate frequency of HPV and EBV infections in OVC and OVH.

Material and Methods Using polymerase chain reaction (PCR), a total of 35 formalin-fixed paraffin-embedded (FFPE) tissue samples, including 27 OVC samples and 8 OVH samples, were investigated for HPV and EBV infection. HeLa and B95-8 cell lines were used as positive controls of HPV and EBV PCR, respectively.

Results All OVC and OVH samples show a positivity to GAPDH, whereas neither HPV nor EBV PCR products was detected in both OVC and OVH samples.

Conclusions In summary, our study demonstrated that HPV and EBV are not involved in pathogenesis of OVC and OVH. Other etiologic factors contributing to OVC and OVH need to be further clarified.

Keywords

oral verrucous squamous cell carcinoma - oral verrucous carcinoma - oral verrucous hyperplasia - HPV - EBV


Publication History

Article published online:
02 December 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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