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CC BY 4.0 · Eur J Dent 2022; 16(02): 403-413
DOI: 10.1055/s-0041-1736291
Original Article

Analyses of Bone Regeneration Capacity of Freeze-Dried Bovine Bone and Combined Deproteinized–Demineralized Bovine Bone Particles in Mandibular Defects: The Potential Application of Biological Forms of Bovine-Bone Filler

David Buntoro Kamadjaja
1   Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Handhito Satriyo
1   Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Aris Setyawan
1   Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Yeni Dian Lesmaya
1   Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Jefry Wahyudi Safril
1   Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Ni Putu Mira Sumarta
1   Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Andra Rizqiawan
1   Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Coen Pramono Danudiningrat
1   Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Ta To Tran
2   Department of Oral Surgery, Faculty of Dentistry, Van Lang University, Ho Chi Minh, Vietnam
› Institutsangaben Funding This work was financially supported by The Ministry of Research Technology and Higher Education, Republic of Indonesia under the contract with Universitas Airlangga Surabaya, Indonesia (grant no. 352/UN3.14/PT/2020).
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Abstract

Objective This study aimed to evaluate bone regeneration capacity of FDBX granules compared to composite DBBM/DFDBX granules for filling of bone defect in rabbit mandible.

Material and Methods Critical size defects were created in 45 rabbits' mandible. The defect in the control group is left untreated, while in other groups the defects were filled with FDBX granules and composite DBBM/DFDBX granules, respectively. Specimens were collected at 2, 4, and 8 weeks for histology and immunohistochemical analyses. Significant difference is set at p-value < 0.05.

Results The osteoblast-osteoclast quantification, osteoblast expression of Runx2, alkaline phosphatase, collagen-I, and osteocalcin, and osteoclast expression of receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG) in FDBX groups were statistically comparable (p > 0.05) with the composite group, while OPG/RANKL ratio, bone healing scores, and trabecular area were significantly higher (p < 0.05) in the composite compared to FDBX group.

Conclusion Composite DBBM/DFDBX granules, within the limitation of this study, has better bone forming capacity than FDBX granules for filling of bone defects in the mandible.

Keywords

bovine - deproteinized - demineralized - osteoconductive - osteoinductive - bone filler - innovation


Publikationsverlauf

Artikel online veröffentlicht:
23. November 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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