Analyses of Bone Regeneration Capacity of Freeze-Dried Bovine Bone and Combined Deproteinized–Demineralized Bovine Bone Particles in Mandibular Defects: The Potential Application of Biological Forms of Bovine-Bone Filler

David Buntoro Kamadjaja; Handhito Satriyo; Aris Setyawan; Yeni Dian Lesmaya; Jefry Wahyudi Safril; Ni Putu Mira Sumarta; Andra Rizqiawan; Coen Pramono Danudiningrat; Ta To Tran

doi:10.1055/s-0041-1736291

European Journal of Dentistry, Inhaltsverzeichnis

CC BY 4.0 · Eur J Dent 2022; 16(02): 403-413
DOI: 10.1055/s-0041-1736291

Original Article

Analyses of Bone Regeneration Capacity of Freeze-Dried Bovine Bone and Combined Deproteinized–Demineralized Bovine Bone Particles in Mandibular Defects: The Potential Application of Biological Forms of Bovine-Bone Filler

Autoren

David Buntoro Kamadjaja

¹Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
Handhito Satriyo

¹Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
Aris Setyawan

¹Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
Yeni Dian Lesmaya

¹Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
Jefry Wahyudi Safril

¹Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
Ni Putu Mira Sumarta

¹Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
Andra Rizqiawan

¹Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
Coen Pramono Danudiningrat

¹Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
Ta To Tran

²Department of Oral Surgery, Faculty of Dentistry, Van Lang University, Ho Chi Minh, Vietnam

Abstract

Objective This study aimed to evaluate bone regeneration capacity of FDBX granules compared to composite DBBM/DFDBX granules for filling of bone defect in rabbit mandible.

Material and Methods Critical size defects were created in 45 rabbits' mandible. The defect in the control group is left untreated, while in other groups the defects were filled with FDBX granules and composite DBBM/DFDBX granules, respectively. Specimens were collected at 2, 4, and 8 weeks for histology and immunohistochemical analyses. Significant difference is set at p-value < 0.05.

Results The osteoblast-osteoclast quantification, osteoblast expression of Runx2, alkaline phosphatase, collagen-I, and osteocalcin, and osteoclast expression of receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG) in FDBX groups were statistically comparable (p > 0.05) with the composite group, while OPG/RANKL ratio, bone healing scores, and trabecular area were significantly higher (p < 0.05) in the composite compared to FDBX group.

Conclusion Composite DBBM/DFDBX granules, within the limitation of this study, has better bone forming capacity than FDBX granules for filling of bone defects in the mandible.

Keywords

bovine - deproteinized - demineralized - osteoconductive - osteoinductive - bone filler - innovation

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Referenzen

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