Planta Med 2021; 87(15): 1289
DOI: 10.1055/s-0041-1736909
Abstracts
8. Poster Contributions
8.8 Medicinal plants and natural product research on Traditional Medicines

Development and physicochemical characterization of nanoliposomes with incorporated oleocanthal, oleacein, oleuropein and hydroxytyrosol

W Li
1   Institute of Chemical Industry of Forestry Products, CAF, Nanjing, China
2   Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, NKUA, Athens, Greece
3   College of Materials Science and Technology, Beijing Forest University, Beijing, China
,
M Chountoulesi
4   Division of Pharmaceutical Technology, Department of Pharmacy, NKUA, Athens, Greece
,
L Antoniadi
2   Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, NKUA, Athens, Greece
,
A Angelis
2   Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, NKUA, Athens, Greece
,
J Lei
3   College of Materials Science and Technology, Beijing Forest University, Beijing, China
,
M Halabalaki
2   Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, NKUA, Athens, Greece
,
C Demetzos
4   Division of Pharmaceutical Technology, Department of Pharmacy, NKUA, Athens, Greece
,
S Mitakou
2   Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, NKUA, Athens, Greece
,
C Wang
1   Institute of Chemical Industry of Forestry Products, CAF, Nanjing, China
› Institutsangaben
This research was supported by the Fundamental Research Funds for The Central Non-profit Research Institution of CAF (CAFYBB2019GC001-12)
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Oleocanthal (OLEO), Oleacein (OLEA), Hydroxytyrosol (HT) and Oleuropein (OLEU) are high-value secondary metabolites of Olea europaea (Oleaceae) with numerous biological activities. Nevertheless, their absorption during the oral administration or skin applications is limited due to low bioavailability. The incorporation of sensitive compounds in drug delivery nanosystems is a well-established method to overcome degradation and bioavailability problems.

The aim of the present work is the development and physicochemical characterization of nanoliposomes with incorporated OLEO, OLEA, HT and OLEU, after their chromatographic isolation in pure form, from extra virgin olive oil (OLEO, OLEA, HT) or olive leaves (OLEU), following previously developed processes [1] [2].

Each pure compound was incorporated in liposomes, prepared from hydrogenated soya phosphatidylcholine (HSPC) lipid, by thin-film hydration preparation method. Their physicochemical stability of size, polydispersity index (PDI) and ζ-potential was evaluated during storage by light scattering methods.

The physicochemical characterization of liposomes was carried out by FTIR, XRD, TGA, DSC and rheology methods. Their morphology was illustrated by negative-staining TEM and Cryo-TEM, revealing well-dispersed round-shaped vesicles.

According to the results, liposomes of larger sizes exhibited higher encapsulation efficiencies of higher concentrations of compounds. According to release studies in simulated body fluid (SBF), OLEU and HT were released in higher percentage than OLEO and OLEA. Their release exponents indicated a Fickian diffusion, while the release mechanisms were well-fitted with Ritger-Peppas model. Taken into account all the obtained results, the proposed liposomes could be suitable carriers for targeted nutrient delivery in the human body.



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Artikel online veröffentlicht:
13. Dezember 2021

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  • References

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