Planta Med 2021; 87(15): 1294
DOI: 10.1055/s-0041-1736923
Abstracts
8. Poster Contributions
8.8 Medicinal plants and natural product research on Traditional Medicines

Protective effects of Phytohustil® compared to its active ingredient Althaea officinalis L. root extract (STW42) on primary human epidermal keratinocytes

Gollin 1
J Müller
2   PSDC, Bayer Consumer Health, Steigerwald Arzneimittelwerk GmbH, Darmstadt, Germany.
,
O Kelber
2   PSDC, Bayer Consumer Health, Steigerwald Arzneimittelwerk GmbH, Darmstadt, Germany.
,
G A Bonaterra
1   Philipps University Marburg, Marburg, Germany
,
R Kinscherf
1   Philipps University Marburg, Marburg, Germany
› Author AffiliationsJ.M. and OK are employees of Steigerwald Arzneimittelwerk GmbH, Darmstadt. The study was supported by Steigerwald Arzneimittelwerk GmbH, Darmstadt. Arzneimittelwerk GmbH, Darmstadt
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Introduction Root extracts of Althaea officinalis L. (STW42) are used for the treatment of dry cough. Limiting the effect only to its polysaccharide components is not enough to explain its effects [1]. Coumarins and flavonoids, have anti-inflammatory/-oxidative properties and contribute to the beneficial effects of STW42 [1]. Keratinocytes act as the major barrier to agents that cause injury in the oral mucosa. The aim of this project is to investigate the anti-inflammatory/-oxidative properties of Phytohustil® on normal human epidermal keratinocytes (NHEK).

Material and methods NHEK were pre-incubated (5–6h) with Phytohustil®, its excipients, or its API STW42, followed by H2O2 (pro-oxidative) or LPS (pro-inflammatory) and further incubated (24h). Viability was measured by PrestoBlue®. Intracellular ROS were quantified with DCFDA. The release of IL-1β was determined by ELISA.

Results 50-1000µg/ml Phytohustil® or STW42 (24h-48h) revealed no cytotoxicity. Pro-inflammatory stimulation of NHEK with LPS (50μg/ml) induced an IL-1β release by 67% compared to negative control. Pre-treatment with 100-400µg/ml Phytohustil® inhibited the IL-1β release by 18%-35% and STW42 by 35% in comparison with LPS-stimulated NHEK. Pre-treatment (5h) with 1000μg/ml STW42 followed by co-incubation with 400μM H2O2 inhibited the H2O2-induced ROS production by 8- to 17-fold compared to treatment with 400μM H2O2 alone.

Conclusions Our results demonstrate the protective anti-inflammatory properties of Phytohustil® and STW42 and confirm the antioxidative properties of its active ingredient. The in vitro effects of Phytohustil® on NHEK may support its importance as a therapeutic drug for the treatment of irritated oropharyngeal mucosa and for the treatment of the symptomatic dry cough associated with this.



Publication History

Article published online:
13 December 2021

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