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CC BY-NC-ND 4.0 · Arquivos Brasileiros de Neurocirurgia: Brazilian Neurosurgery 2022; 41(04): e316-e323
DOI: 10.1055/s-0041-1740618
Original Article

The Effect of Ascorbic Acid Supplementation on the Time of Healing of Rats Submitted to Neurosurgical Procedures

Efeito da suplementação de ácido ascórbico no tempo de cicatrização de ratos submetidos a procedimentos neurocirúrgicos
Tiago Gonçalves Rosa
1   Hospital Universitário Evangélico Mackenzie, Curitiba, PR, Brazil
› Author Affiliations Funding Coordination for the Improvement of Higher Education Personnel (CAPES).
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Abstract

Introduction Vitamin C is an essential nutrient for both humans and rats and has been noted for its beneficial properties, among them, healing.

Objective To verify the effect of oral and subcutaneous vitamin C supplementation on the healing time of surgical wounds of rats skulls.

Statistical Methodology Thirty male Wistar rats were divided into 3 groups: 10 from the control group (GI), 10 from the group treated with oral vitamin C (GII), and 10 from the group treated with subcutaneous vitamin C (GIII). Vitamin C was administered to GI and GIII animals from the 3rd to the 7th postoperative day, totaling 10 days of administration at a dose of 100 mg/kg/day. On the 4th day of the study, the rats were submitted to a surgical procedure consisting of a 2-cm incision of the skin of the animals' heads and suturing with single stitches. After a determined period, the rats were killed and submitted to the collection of material for study by the picrosirius red technique for the evaluation of collagen types I and III, the degree of hematoxylin and eosin healing, and the rate of contraction of the wound on subsequent days. The results were described in averages, medians, minimum and maximum values, and standard deviations. For the comparison of the three groups, the analysis of variance with one factor (one-way ANOVA) or Kruskal-Wallis non-parametric test was used. The normality of the variables was evaluated by the Shapiro-Wilk test. Values of p < 0.05 indicated statistical significance. The data were analyzed using the IBM SPSS Statistics for Windows, v.20.0. software. (IBM Corp., Armonk, NY, USA).

Results The amount of collagen type III was higher in the groups that received vitamin C, however, without significant difference (n = 0.292). In relation to the rate of contraction of the surgical wound, it was higher in the groups treated with vitamin C, with a significant difference between groups I and II (p = 0.001), and between groups I and III (p < 0.001). No significant difference was found between the groups that were treated with vitamin C (p = 0.227).

Conclusion Healing was more effective in the groups treated with vitamin C than in the group that did not receive vitamin supplementation. There was no significant difference in healing between the groups receiving oral or subcutaneous vitamin C.

Resumo

Introdução A vitamina C é um nutriente essencial tanto para humanos quanto para ratos e tem-se destacado por suas propriedades benéficas, entre elas, a cicatrização.

Objetivo Verificar o efeito da suplementação de vitamina C oral e subcutânea no tempo de cicatrização de feridas cirúrgicas do crânio de ratos.

Metodologia estatística: Foram utilizados 30 ratos Wistar, machos, divididos em 3 grupos, 10 do grupo controle (GI), 10 do grupo tratado com vitamina C oral (GII) e 10 do grupo tratado com vitamina C subcutânea (GIII). A vitamina C foi administrada aos animais de GII e GIII, do 3° dia ao 7° dia pós-operatório, totalizando 10 dias de sua administração, na dose de 100 mg/kg/dia. No 4° dia do estudo, os ratos foram submetidos ao procedimento cirúrgico, que consistiu na incisão de 2 cm da pele da cabeça dos animais e sutura com pontos simples. Após um período determinado, os ratos foram mortos e submetidos a coleta do material para estudo por meio da técnica de picrosirius red para avaliação do colágeno tipos I e III, o grau de cicatrização pela hematoxilina e eosina, e pela taxa de contração da ferida nos dias subsequentes. Os resultados foram descritos por médias, medianas, valores mínimos e máximos, e desvios padrões. Para a comparação dos três grupos, foi usado o modelo de análise da variância com um fator (ANOVA) ou o teste não-paramétrico de Kruskal-Wallis. A condição de normalidade das variáveis foi avaliada pelo teste de Shapiro-Wilk. Valores de p < 0,05 indicaram significância estatística. Os dados foram analisados com o programa computacional IBM SPSS Statistics for Windows, v.20.0. (IBM Corp., Armonk, NY, EUA).

Resultados: A quantidade de colágeno tipo III foi maior nos grupos que receberam vitamina C, porém, sem diferença significativa (n = 0,292). Em relação à taxa de contração da ferida operatória, ela foi maior nos grupos tratados com vitamina C, com diferença significativa entre os grupos I e II (p = 0,001), e entre os grupos I e III (p < 0,001), não sendo encontrada diferença significativa entre os grupos que foram tratados com vitamina C (p = 0,227).

Conclusão A cicatrização foi mais efetiva nos grupos tratados com vitamina C em relação ao grupo que não recebeu suplementação da vitamina. Não houve diferença significativa na cicatrização entre os grupos que receberam a vitamina C oral ou subcutânea.

Keywords

scalp - healing - ascorbic acid - Wistar rats

Palavras-chave

escalpe - cicatrização - ácido ascórbico - ratos Wistar


Publication History

Received: 23 May 2021

Accepted: 30 July 2021

Article published online:
16 December 2022

© 2022. Sociedade Brasileira de Neurocirurgia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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