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DOI: 10.1055/s-0041-1741447
Treatment of Muscle Injury with Stem Cells – Experimental Study in Rabbits
Article in several languages: português | English
Abstract
Objective Histological and macroscopic evaluation of the healing process of acute lesions of the femoral rectus muscle using stem cells derived from adipose tissue-derived stem cells (ADSCs).
Method An experimental study was conducted with 18 hind legs of New Zealand rabbits, which were divided into three study groups according to the intervention to be performed. In group I, no surgical procedure was performed; in group II—SHAN, the experimental lesion was performed without any additional intervention protocol; in group III—Intervention, the addition of ADSCs was performed in the same topography of the experimental lesion. After the proposed period, 2 weeks, the material was collected and submitted to macroscopic and histological evaluation.
Results The quantitative analysis showed that the addition of ADSCs is related to the reduction of inflammatory cells in the 2-week evaluation (164.2 cells in group II – SHAN to 89.62 cells in group III – ADSC). The qualitative analysis of the slides with Picrosirius red, noticed an increase in orange/yellow fibers in group III – ADSC, which evidences a final healing process. The macroscopic evaluation found no difference between the groups.
Conclusion The use of ADSCs in the treatment of acute muscle injury presented histological advantages when compared to their non-use.
Keywords
mesenchymal stem cells - regenerative medicine - muscular diseases - muscles - regeneration* Multicenter study developed in two research centers at Escola Paulista de Medicina, Universidade Federal de São Paulo and at the Department of Biological Sciences, Campus Diadema, UNIFESP, São Paulo, Brazil.
Financial Support
The study was funded by the National Council for Scientific and Technological Development (CNPq) - process number 311237/2018-5.
Authors' Contributions
Each author contributed individually and significantly to the development of the present article.
Publication History
Received: 26 June 2021
Accepted: 20 September 2021
Article published online:
07 February 2022
© 2022. Sociedade Brasileira de Ortopedia e Traumatologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)
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