The Protective Potential of Phyllanthus niruri and Corilagin on Gastric Lesions Induced in Rodents by Different Harmful Agents

Luiz C. Klein-Júnior; Luísa Mota da Silva; Thaise Boeing; Lincon Bordignon Somensi; Ana P. Beber; Jady A. R. Rocha; Amélia T. Henriques; Sérgio F. Andrade; Valdir Cechinel-Filho

doi:10.1055/s-0042-107356

Planta Medica, Inhaltsverzeichnis

Planta Med 2017; 83(01/02): 30-39
DOI: 10.1055/s-0042-107356

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

The Protective Potential of Phyllanthus niruri and Corilagin on Gastric Lesions Induced in Rodents by Different Harmful Agents

Luiz C. Klein-Júnior

¹Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí – UNIVALI, Itajaí, Brazil

²Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí – UNIVALI, Itajaí, Brazil

³Laboratório de Pesquisa em Farmacognosia e Controle de Qualidade de Fitoterápicos, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

,

Luísa Mota da Silva

¹Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí – UNIVALI, Itajaí, Brazil

²Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí – UNIVALI, Itajaí, Brazil

,

Thaise Boeing

¹Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí – UNIVALI, Itajaí, Brazil

²Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí – UNIVALI, Itajaí, Brazil

,

Lincon Bordignon Somensi

¹Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí – UNIVALI, Itajaí, Brazil

²Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí – UNIVALI, Itajaí, Brazil

,

Ana P. Beber

¹Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí – UNIVALI, Itajaí, Brazil

²Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí – UNIVALI, Itajaí, Brazil

,

Jady A. R. Rocha

²Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí – UNIVALI, Itajaí, Brazil

,

Amélia T. Henriques

³Laboratório de Pesquisa em Farmacognosia e Controle de Qualidade de Fitoterápicos, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

,

Sérgio F. Andrade

¹Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí – UNIVALI, Itajaí, Brazil

²Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí – UNIVALI, Itajaí, Brazil

,

Valdir Cechinel-Filho

¹Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí – UNIVALI, Itajaí, Brazil

²Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí – UNIVALI, Itajaí, Brazil

› Institutsangaben

Abstract

The gastroprotective effect of the methanol extract of Phyllantus niruri and its main constituent, corilagin, were studied in vivo. The extract (50, 125, or 250 mg/kg, p. o.) inhibited ethanol-induced lesions in rats by 43 % (p < 0.001), 69 % (p < 0.001), and 99 % (p < 0.001), respectively. It also inhibited the formation of indomethacin-induced gastric ulcers in rats by 80 % (p < 0.01), 89 % (p < 0.01), and 97 % (p < 0.01). A decrease in lipid hydroperoxide levels (p < 0.01) and in myeloperoxidase activity (p < 0.05) evidenced a reduction of oxidative damage and neutrophil infiltration in gastric tissues from ulcerated mice using ethanol/HCl. Potent in vitro free radical scavenger activity (IC₅₀ = 0.07) using the DPPH assay was observed. In contrast, the extract (250 mg/kg, i. d.) did not show antisecretory activity in pylorus-ligated rats, and also failed to inhibit the H⁺,K⁺-ATPase activity in vitro. However, in pylorus-ligated rats, the extract (50, 125, and 250 mg/kg, i. d.) increased adhered mucus content by 22 % (p < 0.05), 28 % (p < 0.01), and 38 % (p < 0.01), respectively. The involvement of prostaglandins, nonprotein endogenous sulfhydryl compounds, α ₂-receptors, and endogenous nitric oxide in the gastroprotection elicited by the extract was also evaluated. Finally, corilagin reduced the lesion area of ethanol-induced gastric ulcers in mice by 88 % (30 mg/kg, p. o.; p < 0.001). Based on these results, it has been concluded that P. niruri methanol extract possesses gastroprotective activity by different and complementary pathways, which together promote an improvement in gastric cytoprotection. The presence of corilagin may partially explain the effectiveness of the extract against gastric damage.

Key words

Phyllanthus niruri - Phyllanthaceae - corilagin - gastric ulcer - prostaglandins - NP-SH compounds - nitric oxide

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Referenzen

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