Gamma-Terpinene Modulation of LPS-Stimulated Macrophages is Dependent on the PGE2/IL-10 Axis

Theresa R. Ramalho; Luciano R. Filgueiras; Maria Talita Pacheco de Oliveira; Ana Luisa de Araujo Lima; Claudio Roberto Bezerra-Santos; Sonia Jancar; Marcia Regina Piuvezam

doi:10.1055/s-0042-107799

Planta Medica, Table of Contents

Planta Med 2016; 82(15): 1341-1345
DOI: 10.1055/s-0042-107799

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Gamma-Terpinene Modulation of LPS-Stimulated Macrophages is Dependent on the PGE2/IL-10 Axis

Theresa R. Ramalho

¹Laboratory of Immunopharmacology, Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brasil

,

Luciano R. Filgueiras

¹Laboratory of Immunopharmacology, Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brasil

,

Maria Talita Pacheco de Oliveira

²Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, Brasil

,

Ana Luisa de Araujo Lima

³Natural Synthetic and Bioactive Products Post-graduation Program, Health Sciences Center, Federal University of Paraíba, João Pessoa, Paraíba, Brasil

,

Claudio Roberto Bezerra-Santos

⁴Laboratory of Immunopharmacology, Department de Physiology and Pathology, Federal University of Paraíba, João Pessoa, Paraíba, Brasil

,

Sonia Jancar

¹Laboratory of Immunopharmacology, Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brasil

,

Marcia Regina Piuvezam

³Natural Synthetic and Bioactive Products Post-graduation Program, Health Sciences Center, Federal University of Paraíba, João Pessoa, Paraíba, Brasil

⁴Laboratory of Immunopharmacology, Department de Physiology and Pathology, Federal University of Paraíba, João Pessoa, Paraíba, Brasil

› Author Affiliations

Abstract

Gamma-terpinene is a monoterpene present in the essential oils of several plants, including those from the Eucalyptus genus. This molecule was recently described as anti-inflammatory and microbiocidal, but little is known about the mechanisms behind its effects. The aim of the present study was to investigate the effect of gamma-terpinene on the lipopolysaccharide-induced production of cytokines by murine peritoneal macrophages. Gamma-terpinene treatment was found to reduce the production of proinflammatory cytokines, such as interleukin-1β and interleukin-6, and enhance that of the anti-inflammatory cytokine interleukin-10. This was accompanied by increased levels of the enzyme cycloxygenase-2 and its product, the lipid mediator prostaglandin E₂. Inhibition of cycloxygenase-2 with nimesulide abolished the potentiating effect of gamma-terpinene on interleukin-10 production. Moreover, nimesulide treatment also abrogated the inhibitory effect of gamma-terpinene on interleukin-1β and interleukin-6. Furthermore, in macrophages from mice deficient in the interleukin-10 gene, gamma-terpinene failed to inhibit interleukin-1β and interleukin-6 production. These results suggest that this monoterpene promotes the prostaglandin E₂/interleukin-10 axis, which inhibits the production of these proinflammatory cytokines.

Key words

gamma-terpinene - macrophage - cycloxygenase-2 - prostaglandin E2

Full Text

References

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