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Planta Med 2017; 83(01/02): 63-69
DOI: 10.1055/s-0042-108203
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

The Hydroalcoholic Extract of Leaves of Mandevilla moricandiana Induces NO-Mediated Vascular Relaxation

Letícia Lima Dias Moreira Ferreira
1   Laboratório Integrado de Pesquisa, Universidade Federal do Rio de Janeiro – Campus Macaé Professor Aloísio Teixeira, Rio de Janeiro, Brazil
,
Márcio Vinícius Gomes
2   Laboratório de Produtos Bioativos, IMCT, Universidade Federal do Rio de Janeiro – Campus Macaé Professor Aloísio Teixeira, Rio de Janeiro, Brazil
,
Bruno Meirelles Paes
1   Laboratório Integrado de Pesquisa, Universidade Federal do Rio de Janeiro – Campus Macaé Professor Aloísio Teixeira, Rio de Janeiro, Brazil
,
Paula Lima do Carmo
1   Laboratório Integrado de Pesquisa, Universidade Federal do Rio de Janeiro – Campus Macaé Professor Aloísio Teixeira, Rio de Janeiro, Brazil
,
Tatiana Ungaretti Paleo Konno
3   Núcleo de Pesquisa em Ecologia e Desenvolvimento Sócio-Ambiental de Macaé, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
,
Francisco de Assis Esteves
3   Núcleo de Pesquisa em Ecologia e Desenvolvimento Sócio-Ambiental de Macaé, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
,
Norberto Peporine Lopes
4   Departamento de Física e Química da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil
,
José Carlos Tomaz
4   Departamento de Física e Química da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil
,
Ivana Correa Ramos Leal
5   Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
,
Denise Oliveira Guimarães
2   Laboratório de Produtos Bioativos, IMCT, Universidade Federal do Rio de Janeiro – Campus Macaé Professor Aloísio Teixeira, Rio de Janeiro, Brazil
,
Michelle Frazão Muzitano
2   Laboratório de Produtos Bioativos, IMCT, Universidade Federal do Rio de Janeiro – Campus Macaé Professor Aloísio Teixeira, Rio de Janeiro, Brazil
,
Juliana Montani Raimundo
1   Laboratório Integrado de Pesquisa, Universidade Federal do Rio de Janeiro – Campus Macaé Professor Aloísio Teixeira, Rio de Janeiro, Brazil
› Author Affiliations
Further Information

Publication History

received 17 December 2015
revised 20 April 2016

accepted 27 April 2016

Publication Date:
10 June 2016 (online)

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Abstract

Natural products extracted from plants represent a valuable source of new bioactive substances. Many studies describe the potential of plant products for the treatment of cardiovascular diseases. Species of the Mandevilla genus have been studied for their biological activities, mainly as antioxidant, anti-inflammatory, and vasorelaxant. However, the phytochemical and pharmacological profiles of Mandevilla moricandiana have not been investigated yet. The aim of this study was to evaluate the vasodilator effect of the hydroalcoholic extract of the leaves of M. moricandiana, as well as its chemical profile. Chemical analysis and quantification of major compounds were performed by HPLC analysis. Total flavonoid content was quantified based on rutin equivalents, and major compounds were identified based on HPLC-DAD-MS analysis. M. moricandiana leaf extract-induced vasodilation was investigated in rat aortic rings precontracted with phenylephrine. The total flavonoids were quantified as 3.25 ± 0.11 % w/w of the hydroalcoholic leaf extract, and HPLC-DAD-MS allowed for the identification of luteolin and quercetin glycosides. The maximal relaxant effect of the hydroalcoholic leaf extract was 86.07 ± 1.68 % at a concentration of 30 µg/mL (p < 0.05; n = 6). The concentration of hydroalcoholic extract of the leaves of M. moricandiana necessary to reduce phenylephrine-induced contractions of the endothelium-intact aorta by 50 % was 0.82 ± 0.10 µg/mL. M. moricandiana leaf extract-induced vasodilation was abolished in aortas pretreated with NG-nitro-L-arginine methyl ester and 1H-[1,2,4]oxadiazolo-[4,3-α]quinoxalin-1-one. In addition, diphenhydramine partially inhibited the effect of the hydroalcoholic extract of the leaves of M. moricandiana. Thus, M. moricandiana-induced relaxation depends on the endothelium and on the activation of the nitric oxide/cyclic GMP pathway, with the involvement of endothelial histamine H1 receptors. Luteolin and quercetin glycosides seem to contribute to the extract activity.

Key words

Mandevilla moricandiana - Apocynaceae - vasodilatation - flavonoids - nitric oxide - rat aorta

Supporting Information

 

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