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Planta Med 2016; 82(15): 1381-1386
DOI: 10.1055/s-0042-108208
Natural Product Chemistry and Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Use of Random Amplified Polymorphic DNA (RAPD) Technique to Study the Genetic Diversity of Eight Aloe Species

Shahira M. Ezzat
Pharmacognosy Department, College of Pharmacy, Cairo University, Cairo, Egypt
,
Abeer M. El Sayed
Pharmacognosy Department, College of Pharmacy, Cairo University, Cairo, Egypt
,
Maha M. Salama
Pharmacognosy Department, College of Pharmacy, Cairo University, Cairo, Egypt
› Author Affiliations
Further Information

Publication History

received 22 March 2016
revised 21 April 2016

accepted 23 April 2016

Publication Date:
23 June 2016 (online)

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Abstract

The genus Aloe comprises over 400 species of flowering succulent plants. Aloe leaves are used in the treatment of asthma, gastrointestinal ulcers, cardiovascular disease, tumors, burns, and diabetes. They are rich in anthraquinones, such as aloin, aloe-emodin, chrysophanol, aloinoside A, and aloinoside B. The various species of Aloe show chemical and morphological similarity and diversity, which depend on the genotype and environmental conditions. In a continuity to our interest in the genus Aloe, this study targets the authentication of eight different Aloe species, Aloe vera (A1), Aloe arborescens (A2), Aloe eru (A3), Aloe grandidentata (A4), Aloe perfoliata (A5), Aloe brevifolia (A6), Aloe saponaria (A7), and Aloe ferox (A8), grown in Egypt by using the technique of random amplified polymorphic DNA. Twelve decamer primers were screened in amplification with genomic DNA extracted from all species, of which five primers yielded species-specific reproducible bands. Out of 156 loci detected, the polymorphic, monomorphic, and unique loci were 107, 26, and 23, respectively. Based on a dendrogram and similarity matrix, the eight Aloe species were differentiated from each other and showed more divergence. Aloe species prevailed similarity coefficients of 54–70 % by which they could be classified into three major groups. Thus, this technique may contribute to the identification of these Aloe species that have great morphological similarity in the Egyptian local markets.

Key words

Aloe species - Xanthorrhoeaceae - authentication - DNA markers - clustering

Supporting Information

 

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