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Planta Med 2016; 82(11/12): 1105-1109
DOI: 10.1055/s-0042-108738
Natural Product Chemistry and Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Efficient Isolation of Dihydropyranocoumarins and Simple Coumarins from Mutellina purpurea Fruits[*]

Krystyna Skalicka-Woźniak
1   Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Lublin, Poland
,
Tomasz Mroczek
1   Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Lublin, Poland
,
Magdalena Walasek
1   Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Lublin, Poland
,
Kazimierz Głowniak
1   Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Lublin, Poland
2   Department of Cosmetology, University of Information Technology and Management in Rzeszow, Rzeszow, Poland
› Author Affiliations
Further Information

Publication History

received 26 February 2016
revised 23 April 2016

accepted 02 May 2016

Publication Date:
10 June 2016 (online)

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Abstract

For the first time, the separation of coumarin derivatives from the petroleum ether extract of the fruits of Mutellina purpurea through high-performance countercurrent chromatography (HPCCC) is described. Four compounds, pteryxin (1; 2.72 mg), hyuganin C (2; 7.94 mg), osthol (3; 4.30 mg), and hyuganin A (4; 3.09 mg), were obtained in a single run following the injection of crude extract (300 mg). Additionally, auraptenol (5) and hyuganin D (6) were identified using LC-ESI-(Q)TOF-MS. The structures of the isolated compounds were elucidated through spectroscopic (NMR and MS) methods. This is apparently the first report of the identification of dihydropyranocoumarins in this plant, and the first time that HPCCC was used to separate them.

Key words

Mutellina purpurea - Apiaceae - high-performance countercurrent chromatography - dihydropyranocoumarins - coumarins - spectroscopic analysis

* Dedicated to Professor Dr. Dr. h. c. mult. Kurt Hostettmann in recognition of his outstanding contribution to natural product research.


Supporting Information

 

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