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Planta Med 2016; 82(17): 1456-1462
DOI: 10.1055/s-0042-109173
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Protective Effects of a Diarylheptanoid from Curcuma comosa Against Hydrogen Peroxide-Induced Astroglial Cell Death

Jaturavit Vattanarongkup
1   Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok, Thailand
,
Pawinee Piyachaturawat
2   Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand
,
Patoomratana Tuchinda
3   Department of Chemistry, Faculty of Science, Mahidol University, Bangkok, Thailand
,
Pimtip Sanvarinda
1   Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok, Thailand
,
Yupin Sanvarinda
1   Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok, Thailand
,
Nattinee Jantaratnotai
1   Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok, Thailand
› Author Affiliations
Further Information

Publication History

received 06 January 2016
revised 16 May 2016

accepted 18 May 2016

Publication Date:
24 June 2016 (online)

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Abstract

Oxidative stress is one of the major mechanisms causing neuronal and astroglial cell death in various neurological disorders such as Alzheimerʼs disease, Parkinsonʼs disease, and brain ischemia. Two diarylheptanoids, (3R)-1,7-diphenyl-(4E,6E)-4,6-heptadien-3-ol (ASPP 049) and (3S)-7-(3,4-dihydroxyphenyl)-1-phenyl-(1E)-1-hepten-3-ol (ASPP 092), isolated from Curcuma comosa were investigated for cytoprotective effects on C6 astroglial cells using hydrogen peroxide (H2O2) exposure as a model of oxidative stress. ASPP 092 demonstrated free radical scavenging activity comparable to that of vitamin C, while ASPP 049 showed no antioxidant activity. Treatment with H2O2 at 400 µM for 12 h caused 79 % C6 astroglial cell death which was significantly reduced to 37 % by pretreatment with ASPP 092 (5 µM). In addition, ASPP 092 attenuated the increase in reactive oxygen species production and the decrease in total glutathione level induced by H2O2. The mechanism of ASPP 092 protection against H2O2-induced apoptotic signaling appeared to involve prevention of increase in the level of phosphorylated p53 and the Bax/Bcl-2 ratio as well as cleaved caspase-3. These findings provide new evidence that the diarylheptanoid ASPP 092 from C. comosa possesses antiapoptotic properties and could be further developed as a potential treatment for oxidative stress-related neuronal diseases.

Key words

Curcuma comosa - Zingiberaceae - apoptosis - C6 astroglia - diarylheptanoid - hydrogen peroxide - oxidative stress
 

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