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Planta Med 2016; 82(17): 1468-1474
DOI: 10.1055/s-0042-110856
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Phenylpyruvic Acid-2-O-β-D-Glucoside Attenuates High Glucose-Induced Apoptosis in H9c2 Cardiomyocytes

Phiwayinkosi Vusi Dludla
1   Biomedical Research and Innovation Platform (BRIP), Medical Research Council (MRC), Tygerberg, South Africa
2   Division of Medical Physiology, Faculty of Health Sciences, Stellenbosch _University, Tygerberg, South Africa
,
Christo John Frederick Muller
1   Biomedical Research and Innovation Platform (BRIP), Medical Research Council (MRC), Tygerberg, South Africa
,
Elizabeth Joubert
3   Post-Harvest and Wine Technology Division, Agricultural Research Council (ARC) Infruitec-Nietvoorbij, Stellenbosch, South Africa
4   Department of Food Science, Stellenbosch University, Stellenbosch, South Africa
,
Johan Louw
1   Biomedical Research and Innovation Platform (BRIP), Medical Research Council (MRC), Tygerberg, South Africa
,
Kwazi Bethuel Gabuza
1   Biomedical Research and Innovation Platform (BRIP), Medical Research Council (MRC), Tygerberg, South Africa
,
Barbara Huisamen
1   Biomedical Research and Innovation Platform (BRIP), Medical Research Council (MRC), Tygerberg, South Africa
2   Division of Medical Physiology, Faculty of Health Sciences, Stellenbosch _University, Tygerberg, South Africa
,
M. Faadiel Essop
5   Cardio-Metabolic Research Group (CMRG), Department of Physiological Sciences, Stellenbosch University, Stellenbosch, South Africa
,
Rabia Johnson
1   Biomedical Research and Innovation Platform (BRIP), Medical Research Council (MRC), Tygerberg, South Africa
› Author Affiliations
Further Information

Publication History

received 13 December 2015
revised 01 June 2016

accepted 14 June 2016

Publication Date:
12 July 2016 (online)

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Abstract

Chronic hyperglycemia is closely associated with impaired substrate metabolism, dysregulated mitochondrial membrane potential, and apoptosis in the diabetic heart. As adult cardiomyocytes display a limited capacity to regenerate following an insult, it is essential to protect the myocardium against the detrimental effects of chronic hyperglycemia. This study therefore investigated whether phenylpyruvic acid-2-O-β-D-glucoside, present in Aspalathus linearis (rooibos), is able to attenuate hyperglycemia-induced damage in H9c2 cardiomyocytes. H9c2 cardiomyocytes were exposed to a high glucose concentration (33 mM) prior to treatment with phenylpyruvic acid-2-O-β-D-glucoside (1 µM), metformin (1 µM), or a combination of phenylpyruvic acid-2-O-β-D-glucoside and metformin (both at 1 µM). Our data revealed that high glucose exposure increased cardiac free fatty acid uptake and oxidation, mitochondrial membrane potential, and apoptosis (caspase 3/7 activity and TUNEL), and decreased the Bcl2/Bax protein expression ratio. Phenylpyruvic acid-2-O-β-D-glucoside treatment, alone or in combination with metformin, attenuated these glucose-induced perturbations, confirming its protective effect in H9c2 cardiomyocytes exposed to chronic hyperglycemia.

Key words

phenylpyruvic acid-2-O-β-D-glucoside - hyperglycemia - fatty acid uptake and oxidation - myocardial apoptosis

Supporting Information

 

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