Curcumin Encapsulated into Methoxy Poly(Ethylene Glycol) Poly(ε-Caprolactone) Nanoparticles Increases Cellular Uptake and Neuroprotective Effect in Glioma Cells

Gregory Marslin; Bruno Filipe Carmelino Cardoso Sarmento; Gregory Franklin; José Alberto Ribeiro Martins; Carlos Jorge Ribeiro Silva; Andreia Ferreira Castro Gomes; Marisa Passos Sárria; Olga Maria Fernandes Pereira Coutinho; Alberto Carlos Pires Dias

doi:10.1055/s-0042-112030

Planta Medica, Table of Contents

Planta Med 2017; 83(05): 434-444
DOI: 10.1055/s-0042-112030

Formulation and Delivery Systems of Natural Products

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Curcumin Encapsulated into Methoxy Poly(Ethylene Glycol) Poly(ε-Caprolactone) Nanoparticles Increases Cellular Uptake and Neuroprotective Effect in Glioma Cells

Authors

Gregory Marslin

¹Centre for the Research and Technology of Agro-Environment and Biological Sciences (CITAB-UM), AgroBioPlant Group, Department of Biology, University of Minho, Braga, Portugal
Bruno Filipe Carmelino Cardoso Sarmento

²CESPU, IINFACTS – Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Instituto Superior de Ciências da Saúde-Norte, Gandra-PRD, Portugal

³INEB – Instituto de Engenharia Biomédica, University of Porto, Porto, Portugal
Gregory Franklin

⁴Department of Integrative Plant Biology, Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland
José Alberto Ribeiro Martins

⁵Department of Chemistry, University of Minho, Braga, Portugal
Carlos Jorge Ribeiro Silva

⁵Department of Chemistry, University of Minho, Braga, Portugal
Andreia Ferreira Castro Gomes

⁶CBMA – Centro de Biologia Molecular e Ambiental, Department of Biology, University of Minho, Braga, Portugal
Marisa Passos Sárria

⁶CBMA – Centro de Biologia Molecular e Ambiental, Department of Biology, University of Minho, Braga, Portugal

⁷INL – International Iberian Nanotechnology Laboratory, Braga, Portugal
Olga Maria Fernandes Pereira Coutinho

¹Centre for the Research and Technology of Agro-Environment and Biological Sciences (CITAB-UM), AgroBioPlant Group, Department of Biology, University of Minho, Braga, Portugal
Alberto Carlos Pires Dias

¹Centre for the Research and Technology of Agro-Environment and Biological Sciences (CITAB-UM), AgroBioPlant Group, Department of Biology, University of Minho, Braga, Portugal

Abstract

Curcumin is a natural polyphenolic compound isolated from turmeric (Curcuma longa) with well-demonstrated neuroprotective and anticancer activities. Although curcumin is safe even at high doses in humans, it exhibits poor bioavailability, mainly due to poor absorption, fast metabolism, and rapid systemic elimination. To overcome these issues, several approaches, such as nanoparticle-mediated targeted delivery, have been undertaken with different degrees of success. The present study was conducted to compare the neuroprotective effect of curcumin encapsulated in poly(ε-caprolactone) and methoxy poly(ethylene glycol) poly(ε-caprolactone) nanoparticles in U251 glioblastoma cells. Prepared nanoparticles were physically characterized by laser doppler anemometry, transmission electron microscopy, and X-ray diffraction. The results from laser doppler anemometry confirmed that the size of poly(ε-caprolactone) and poly(ethylene glycol) poly(ε-caprolactone) nanoparticles ranged between 200–240 nm for poly(ε-caprolactone) nanoparticles and 30–70 nm for poly(ethylene glycol) poly(ε-caprolactone) nanoparticles, and transmission electron microscopy images revealed their spherical shape. Treatment of U251 glioma cells and zebrafish embryos with poly(ε-caprolactone) and poly(ethylene glycol) poly(ε-caprolactone) nanoparticles loaded with curcumin revealed efficient cellular uptake. The cellular uptake of poly(ethylene glycol) poly(ε-caprolactone) nanoparticles was higher in comparison to poly(ε-caprolactone) nanoparticles. Moreover, poly(ethylene glycol) poly(ε-caprolactone) di-block copolymer-loaded curcumin nanoparticles were able to protect the glioma cells against tBHP induced-oxidative damage better than free curcumin. Together, our results show that curcumin-loaded poly(ethylene glycol) poly(ε-caprolactone) di-block copolymer nanoparticles possess significantly stronger neuroprotective effect in U251 human glioma cells compared to free curcumin and curcumin-loaded poly(ε-caprolactone) nanoparticles.

Key words

curcumin - PEGylated nanoparticles - neuroprotection - cellular uptake - zebrafish

Full Text

References

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