Suppression of Cartilage Degradation by Zingerone Involving the p38 and JNK MAPK Signaling Pathway

Jetsada Ruangsuriya; Piyaporn Budprom; Nawarat Viriyakhasem; Patiwat Kongdang; Chatchadawalai Chokchaitaweesuk; Nutnicha Sirikaew; Siriwadee Chomdej; Korakot Nganvongpanit; Siriwan Ongchai

doi:10.1055/s-0042-113387

Planta Medica, Table of Contents

Planta Med 2017; 83(03/04): 268-276
DOI: 10.1055/s-0042-113387

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Suppression of Cartilage Degradation by Zingerone Involving the p38 and JNK MAPK Signaling Pathway

Jetsada Ruangsuriya

¹Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

,

Piyaporn Budprom

¹Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

,

Nawarat Viriyakhasem

¹Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

,

Patiwat Kongdang

¹Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

,

Chatchadawalai Chokchaitaweesuk

¹Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

,

Nutnicha Sirikaew

¹Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

,

Siriwadee Chomdej

²Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand

,

Korakot Nganvongpanit

³Animal Bone and Joint Research Laboratory, Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand

,

Siriwan Ongchai

⁴Thailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry and the Center of Excellence for Innovation in Chemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

› Author Affiliations

Abstract

Zingerone, an active compound that is present in cooked ginger, has been claimed to be a bioactive ingredient that holds the potential of preventing and/or treating diseases involving inflammation. In this study, zingerone was used to discover its properties against joint inflammation using interleukin-1β-induced osteoarthritis in cartilage explant and cell culture models. Zingerone was supplemented into the cartilage explant and cell culture media at different concentrations along with the presence of interleukin-1β, an inducer of osteoarthritis. Markers indicating cartilage degradation, inflammation, and the signaling molecules involved in the inflammatory induction were investigated. Diacerien, an anti-osteoarthritic drug, was used as a positive control. Zingerone at a concentration of 40 µM reduced the level of matrix metalloproteinase-13 to about 31.95 ± 4.33 % compared with the interleukin-1β-treated group and halted cartilage explant degradation as indicated by reducing the accumulative release of sulfated glycosaminoglycans by falling to the control concomitantly with an elevation of the remaining contents of uronic acid and collagen in the explant tissues when zingerone was added. In the SW1353 cell line model, zingerone efficiently suppressed the expression of TNF-α, interleukin-6, and interleukin-8 mRNA levels and tended to reduce the levels of both p38 and c-Jun N-terminal kinase phosphorylation. From the results of this study, it can be concluded that zingerone potentially reduced cartilage degradation, which is partially involved in p38 and c-Jun N-terminal kinases of the mitogen activator protein kinase signaling pathway leading to the reduction of proinflammatory cytokine amplification effects and cartilage-degrading enzyme syntheses. This finding supports the contention that ginger holds positive pharmaceutical effects against osteoarthritis.

Key words

Zingiberaceae - Zingiber officinale - ginger - zingerone - osteoarthritis - cartilage explants - chondroprotection

Full Text

References

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