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Horm Metab Res 2016; 48(10): 682-686
DOI: 10.1055/s-0042-114039
Endocrine Research
© Georg Thieme Verlag KG Stuttgart · New York

Effect of Diabetes on Circulating Pancreatic Hormones in Pregnant Rats and Their Offspring

I. L. Iessi
1   Laboratory of Experimental Research in Gynecology and Obstetrics, Program of Gynecology, Obstetrics and Mastology Graduate Course, Botucatu Medical School, Unesp Univ Estadual Paulista, Botucatu, São Paulo State, Brazil
2   Laboratory of Cellular and Metabolic Research, Department of Biomedical Science, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
,
Y. K. Sinzato
1   Laboratory of Experimental Research in Gynecology and Obstetrics, Program of Gynecology, Obstetrics and Mastology Graduate Course, Botucatu Medical School, Unesp Univ Estadual Paulista, Botucatu, São Paulo State, Brazil
,
F. Q. Gallego
1   Laboratory of Experimental Research in Gynecology and Obstetrics, Program of Gynecology, Obstetrics and Mastology Graduate Course, Botucatu Medical School, Unesp Univ Estadual Paulista, Botucatu, São Paulo State, Brazil
,
J. H. Nielsen
2   Laboratory of Cellular and Metabolic Research, Department of Biomedical Science, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
,
D. C. Damasceno
1   Laboratory of Experimental Research in Gynecology and Obstetrics, Program of Gynecology, Obstetrics and Mastology Graduate Course, Botucatu Medical School, Unesp Univ Estadual Paulista, Botucatu, São Paulo State, Brazil
› Author Affiliations
Further Information

Publication History

received 31 May 2016

accepted 27 July 2016

Publication Date:
15 September 2016 (online)

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Abstract

The aim of the study was to investigate the role of diabetic intrauterine environment on circulating insulin, glucagon, and somatostatin levels in pregnant rats, fetuses, and offspring. Diabetes was induced in female Wistar rats by streptozotocin at birth or as adult and the animals were assigned into: control (C); mildly diabetic (MD); and severely diabetic (SD). The rats were mated and distributed into 2 subgroups: euthanasia at day 21 of pregnancy and at day 10 postpartum. Both MD and SD dams showed impaired oral glucose tolerance. SD dams had lower body weight and insulin levels compared to C and MD dams. SD fetuses presented hyperglycemia and reduction of insulin and glucagon levels compared to C and MD fetuses. SD newborns had diminished total pancreatic insulin and plasma somatostatin compared to the other groups. MD dams and fetuses had lower glucagon and somatostatin levels compared to C dams. MD offspring had maintained lower somatostatin levels to neonatal period. Diabetes causes alterations in circulating levels of pancreatic hormones in the mother and offspring.

Key words

diabetes - glucagon - insulin - somatostatin - pregnancy - streptozotocin
 

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