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Drug Res (Stuttg) 2017; 67(03): 149-155
DOI: 10.1055/s-0042-118448
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Novel 99mTc-Radiolabeled Anionic Linear Globular PEG-Based Dendrimer-Chlorambucil: Non-Invasive Method for In-Vivo Biodistribution

Seyedeh Masoomeh Ghoreishi
1   Faculty of Pharmacy, Department of Radiopharmacy, Tehran University of Medical Sciences, Tehran, Iran
,
Ali khalaj
1   Faculty of Pharmacy, Department of Radiopharmacy, Tehran University of Medical Sciences, Tehran, Iran
,
Ahmad Bitarafan-Rajabi
2   Department of Nuclear Medicine, Cardiovascular Intervention Research Center, Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran
,
Amir Darbandi Azar
2   Department of Nuclear Medicine, Cardiovascular Intervention Research Center, Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran
,
Mehdi Shafiee Ardestani
1   Faculty of Pharmacy, Department of Radiopharmacy, Tehran University of Medical Sciences, Tehran, Iran
,
Artin Assadi
1   Faculty of Pharmacy, Department of Radiopharmacy, Tehran University of Medical Sciences, Tehran, Iran
› Author Affiliations
Further Information

Publication History

received 23 March 2016

accepted 09 May 2016

Publication Date:
19 December 2016 (online)

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Abstract

Chlorambucil (CBL) is an alkylating agent, which widely use in the treatment of various types of tumors. The main purpose of this study is to evaluate the in-vivo biodistribution of CBL conjugated to the anionic dendrimer, which has a great ability to labeled with 99mTc through binding to carboxylate terminate groups. Whole body scans were used to analyze the percentage of the injected dose in different times. Radiochemical purity (RCP) and in-vivo biodistribution were also calculated with the SPECT/CT instrument. Our study proposes a new method for RCP determination and shows that this carrier is a promising agent to complex with the 99mTc and biological assessment.

Key words

dendrimer - chlorambucil - labeling - region-of-interest
 

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