Limonene Enhances the cAMP Response Element (CRE)-Dependent Transcriptional Activity Activated via Adenosine A2 A Receptor in a Neural-Crest Derived Cell Line, PC-12

Jiro Takito; Masaki Ota; Chisato Oba; Hironori Fujiwara; Kiyoshi Murata; Kikuji Yamaguchi; Nobuyuki Uozumi; Masanori Nakamura; Hiroshi Inomata; Yasushi Ohizumi

doi:10.1055/s-0042-118778

Planta Medica International Open, Table of Contents

Planta Medica International Open 2016; 3(03): e60-e62
DOI: 10.1055/s-0042-118778

Letter

Georg Thieme Verlag KG Stuttgart · New York

Limonene Enhances the cAMP Response Element (CRE)-Dependent Transcriptional Activity Activated via Adenosine A2 A Receptor in a Neural-Crest Derived Cell Line, PC-12

Jiro Takito^*

¹Department of Anti-Dementia Functional Food Development, Graduate School of Engineering, Tohoku University, Aoba, Aramaki, Sendai, Japan

²Department of Oral Anatomy and Developmental Biology, School of Dentistry, Showa University, Hatanodai, Shinagawa, Tokyo, Japan

,

Masaki Ota

³Research Center of Supercritical Fluid Technology, Tohoku University, Aoba-ku, Aramaki, Sendai, Japan

,

Chisato Oba

³Research Center of Supercritical Fluid Technology, Tohoku University, Aoba-ku, Aramaki, Sendai, Japan

,

Hironori Fujiwara

¹Department of Anti-Dementia Functional Food Development, Graduate School of Engineering, Tohoku University, Aoba, Aramaki, Sendai, Japan

,

Kiyoshi Murata

⁴Japan Royal Jelly (JRJ) Co. Ltd., R&D Center, Ganbara, Kami-machi, Kami-gun, Miyagi, Japan

,

Kikuji Yamaguchi^*

¹Department of Anti-Dementia Functional Food Development, Graduate School of Engineering, Tohoku University, Aoba, Aramaki, Sendai, Japan

⁵Japan Royal Jelly (JRJ) Co. Ltd., Kyobashi, Chuo-ku, Tokyo, Japan

,

Nobuyuki Uozumi

⁶Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba, Aramaki, Sendai, Japan

,

Masanori Nakamura

²Department of Oral Anatomy and Developmental Biology, School of Dentistry, Showa University, Hatanodai, Shinagawa, Tokyo, Japan

,

Hiroshi Inomata

³Research Center of Supercritical Fluid Technology, Tohoku University, Aoba-ku, Aramaki, Sendai, Japan

,

Yasushi Ohizumi^*

¹Department of Anti-Dementia Functional Food Development, Graduate School of Engineering, Tohoku University, Aoba, Aramaki, Sendai, Japan

⁷Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Suruga-ku, Shizuoka, Japan

⁸Kansei Fukushi Research Center, Tohoku Fukushi University, Kunimi, Aoba-ku, Sendai, Japan

› Author Affiliations

Abstract

Prevention and treatment of Alzheimerʼs disease are urgent problems for elderly people in developed countries. To seek useful compounds that treat, delay the onset, or prevent the development of Alzheimerʼs disease, we screened natural resources using the cyclic AMP response element-dependent transcription in the PC12 cell line. We previously found that honey bee royal jelly stimulates the cyclic AMP response element-dependent transcription activity in PC12 cells. Here, we report that R-limonene, a monoterpene, enhanced the royal jelly-induced cyclic AMP response element-dependent transcription activity, whereas R-limonene showed no effect in absence of royal jelly. The enhancement was inhibited by ZM241385, an antagonist for adenosine A2 A receptor. R-limonene also enhanced the cyclic AMP response element-dependent transcription activity activated by adenosine and AMP. Thus, R-limonene represents a novel type of modulator that enhances the CRE-dependent transcription via adenosine A2 A receptor pathway.

Key words

adenosine receptor - CRE-dependent transcription - limonene - memory - royal jelly

Full Text

References

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