Malondialdehyde Level and Tissue Apoptosis Count as an Early-Detection Marker of Oral Potentially Malignant Disorders

Amalia Yosi Firdausa; Sally Salsabila Ahimsa; Rafdan Affan Ahmada; Natasya Fauzia Sukmawati; Diah Savitri Ernawati; Adiastuti Endah Parmadiati; Bagus Soebadi; Desiana Radithia; Saka Winias; Fatma Yasmin Mahdani; Riyan Iman Marsetyo; Rosnah Binti Zain; Nurina Febriyanti Ayuningtyas

doi:10.1055/s-0042-1743154

European Journal of Dentistry, Table of Contents

CC BY 4.0 · Eur J Dent 2023; 17(01): 155-160
DOI: 10.1055/s-0042-1743154

Original Article

Malondialdehyde Level and Tissue Apoptosis Count as an Early-Detection Marker of Oral Potentially Malignant Disorders

Amalia Yosi Firdausa

¹Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia

,

Sally Salsabila Ahimsa

¹Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia

,

Rafdan Affan Ahmada

¹Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia

,

Natasya Fauzia Sukmawati

¹Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia

,

Diah Savitri Ernawati

²Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya-Indonesia

,

Adiastuti Endah Parmadiati

²Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya-Indonesia

,

Bagus Soebadi

²Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya-Indonesia

,

Desiana Radithia

²Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya-Indonesia

,

Saka Winias

²Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya-Indonesia

,

Fatma Yasmin Mahdani

²Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya-Indonesia

,

Riyan Iman Marsetyo

²Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya-Indonesia

,

Rosnah Binti Zain

³Faculty of Dentistry, MAHSA University, Bandar Saujana Putra, Jenjarom Selangor, Malaysia

⁴Oral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, Malaya, Malaysia

,

Nurina Febriyanti Ayuningtyas

²Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya-Indonesia

› Author Affiliations

Abstract

Objectives The malondialdehyde (MDA) level and TA count represent the progression of oral potentially malignant disorders (OPMD) to malignancy and thus may be used as an indicator of oral epithelial dysplasia (OED). This study aimed to determine the MDA level and tissue apoptosis (TA) count in oropharyngeal tissue of Wistar rats exposed to sidestream cigarette smoke.

Materials and Methods Wistar rats were divided into three groups: T4 group (4-week cigarette smoke exposure), T8 group (8-week cigarette smoke exposure), and control group, which was not exposed to cigarette smoke. The oropharyngeal tissue of the rats from each group was examined histopathologically to count the number of apoptotic cells, and then the blood serum was made to measure the MDA level.

Statistical Analysis Bonferroni test was performed to see the differences in each group for MDA level. While the data from tissue apoptosis were analyzed using Mann-Whitney U test for the significance. All data were considered significant if p < 0.05.

Results The MDA level and TA count increased as the duration of cigarette smoke exposure increased. In the T8 group, the MDA level and TA count were significantly higher compared with the T4 and control groups with a p-value < 0.05.

Conclusions Exposure to sidestream cigarette smoke increased the TA count and MDA level in the oropharyngeal tissue of Wistar rats. The TA count and MDA level may be used as markers of OPMD.

Keywords

tobacco addiction - apoptosis index - malondialdehyde - oral epithelial dysplasia - oral potentially malignant disorder - cancer

Full Text

References

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