PDF herunterladen
CC BY 4.0 · Glob Med Genet 2022; 09(02): 182-184
DOI: 10.1055/s-0042-1743260
Rapid Communication

Nucleotide Sequence Sharing between the Human Genome and Primers for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Detection

Darja Kanduc 
1   Department of Biosciences, Biotechnologies, and Biopharmaceutics, University of Bari, Bari, Italy
› Institutsangaben Funding None.
› Weitere Informationen
Auch verfügbar auf
   Lizenzen und Reprints

Abstract

This study shows that oligonucleotide sequences are shared between the human genome and primers that have been proposed/used for SARS-CoV-2 detection by polymerase chain reaction (PCR). The high level of sharing (namely, up to 19mer with a maximum number of gaps equal to 2) might bear implications for the diagnostic validity of SARS-CoV-2 detection by PCR.

Keywords

PCR primers - SARS-CoV-2 detection - false positives


Publikationsverlauf

Eingereicht: 29. November 2021

Angenommen: 29. Dezember 2021

Artikel online veröffentlicht:
08. März 2022

© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

  • References

  • 1 Kanduc D. Rare human codons and HCMV translational regulation. J Mol Microbiol Biotechnol 2017; 27 (04) 213-216
    CrossrefPubMedSuche in Google Scholar
  • 2 Kanduc D. Human codon usage: the genetic basis of pathogen latency. Glob Med Genet 2021; 8 (03) 109-115
    Thieme ConnectPubMedSuche in Google Scholar
  • 3 Keita AK, Koundouno FR, Faye M. et al. Resurgence of Ebola virus in 2021 in guinea suggests a new paradigm for outbreaks. Nature 2021; 597 (7877): 539-543
    CrossrefPubMedSuche in Google Scholar
  • 4 Dayama P, Sampath K. Dengue disease outbreak detection. Stud Health Technol Inform 2014; 205: 1105-1109
    PubMedSuche in Google Scholar
  • 5 Wu D, Wu T, Liu Q, Yang Z. The SARS-CoV-2 outbreak: what we know. Int J Infect Dis 2020; 94: 44-48
    CrossrefPubMedSuche in Google Scholar
  • 6 Patriquin G, Davidson RJ, Hatchette TF. et al. Generation of false-positive SARS-CoV-2 antigen results with testing conditions outside manufacturer recommendations: a scientific approach to pandemic misinformation. Microbiol Spectr 2021; 9 (02) e0068321
    CrossrefPubMedSuche in Google Scholar
  • 7 Benoit J, Benoit SW, Lippi G, Henry BM. False negative RT-PCR or false positive serological testing in SARS-CoV-2 diagnostics? Navigating between Scylla and Charybdis to prevent misclassification bias in COVID-19 clinical investigations. Diagnosis (Berl) 2020; 7 (04) 405-407
    CrossrefPubMedSuche in Google Scholar
  • 8 Verna R, Alallon W, Murakami M. et al. Analytical performance of COVID-19 detection methods (RT-PCR): scientific and societal concerns. Life (Basel) 2021; 11 (07) 660
    Suche in Google Scholar
  • 9 Corman VM, Landt O, Kaiser M. et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill 2020; 25 (03) 2000045
    CrossrefPubMedSuche in Google Scholar
  • 10 Surkova E, Nikolayevskyy V, Drobniewski F. False-positive COVID-19 results: hidden problems and costs. Lancet Respir Med 2020; 8 (12) 1167-1168
    CrossrefPubMedSuche in Google Scholar
  • 11 Eurosurveillance Editorial Team. Response to retraction request and allegations of misconduct and scientific flaws. Euro Surveill 2021; 26 (05) 2102041
    CrossrefPubMedSuche in Google Scholar
  • 12 Kanduc D. From anti-severe acute respiratory syndrome coronavirus 2 immune response to cancer onset via molecular mimicry and cross-reactivity. Glob Med Genet 2021; 8 (04) 176-182
    Thieme ConnectPubMedSuche in Google Scholar
  • 13 Kanduc D. Thromboses and hemostasis disorders associated with coronavirus disease 2019: the possible causal role of cross-reactivity and immunological imprinting. Glob Med Genet 2021; 8 (04) 162-170
    Thieme ConnectPubMedSuche in Google Scholar
  • 14 Kanduc D. From anti-SARS-CoV-2 immune responses to COVID-19 via molecular mimicry. Antibodies (Basel) 2020; 9 (03) 33
    CrossrefPubMedSuche in Google Scholar
  • 15 Kanduc D. From anti-SARS-CoV-2 immune response to the cytokine storm via molecular mimicry. Antibodies (Basel) 2021; 10 (04) 36
    CrossrefPubMedSuche in Google Scholar
  • 16 Altschul SF, Madden TL, Schäffer AA. et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997; 25 (17) 3389-3402
    Suche in Google Scholar
  • 17 Boratyn GM, Thierry-Mieg J, Thierry-Mieg D, Busby B, Madden TL. Magic-BLAST, an accurate RNA-seq aligner for long and short reads. BMC Bioinformatics 2019; 20 (01) 405
    CrossrefPubMedSuche in Google Scholar
  • 18 Gadkar VJ, Goldfarb DM, Young V. et al. Development and validation of a new triplex real-time quantitative reverse Transcriptase-PCR assay for the clinical detection of SARS-CoV-2. Mol Cell Probes 2021; 58: 101744
    CrossrefPubMedSuche in Google Scholar
  • 19 Qasem A, Shaw AM, Elkamel E, Naser SA. Coronavirus disease 2019 (COVID-19) diagnostic tools: a focus on detection technologies and limitations. Curr Issues Mol Biol 2021; 43 (02) 728-748
    CrossrefPubMedSuche in Google Scholar
  • 20 Uniprot. Accessed November 2021 at: https://www.uniprot.org/uniprot/?query=proteome:UP000464024&sort=score