Open Access
CC BY 4.0 · Eur J Dent 2023; 17(02): 319-329
DOI: 10.1055/s-0042-1745768
Original Article

Bone Remodeling in Mandible of Wistar Rats with Diabetes Mellitus and Osteoporosis

Nike Hendrijantini
1   Department of Prosthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Yonatan Christian Suisan
2   Resident of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Rizko Wira Artha Megantara
2   Resident of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Bambang Agustono Satmoko Tumali
1   Department of Prosthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Mefina Kuntjoro
1   Department of Prosthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Muhammad Dimas Aditya Ari
1   Department of Prosthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Ratri Maya Sitalaksmi
1   Department of Prosthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
3   Division for Globalization Initiative, Graduate School of Dentistry, Tohoku University, Sendai, Japan
› Institutsangaben

Funding This research was supported by Universitas Airlangga International Collaboration Research Grant with number 212/UN3/2021.
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Abstract

Objectives This study aimed to determine some of bone molecular expressions and its possible bone remodeling pathway between diabetes mellitus (DM) and osteoporosis model in the mandibular bone of Wistar rats.

Materials and Methods Twenty-seven female Wistar rats were divided randomly into control and treatment groups. Treatment groups were injected with streptozotocin intraperitoneally to induce DM (P1) and underwent bilateral ovariectomy to generate osteoporosis (P2). All groups were terminated after 12 weeks. Immunohistochemical and hematoxylin–eosin staining were performed to determine the expression of Runt-related transcription factor 2 (RUNX2), Osterix, vascular endothelial growth factor (VEGF), receptor activator of nuclear factor κB ligand (RANKL), osteoprotegerin (OPG), tartrate-resistant acid phosphatase (TRAP), and observed the osteoblast and osteoclast. Statistical analysis was performed using one-way analysis of variance.

Results The lowest mean of RUNX2 and VEGF expression was found in the P2 group. The lowest mean of Osterix expression was found in the P1 group. Both P1 and P2 groups of osteoblast/osteoclast ratio were decreased. There were no significant differences in the expression of TRAP between all groups; however, increased expression of RANKL/OPG ratio was only found in the P2 group.

Conclusion DM and osteoporosis induce changes in the bone remodeling pathway which are represented by a decrease in osteoblast biomarkers and an increase in osteoclast biomarkers.



Publikationsverlauf

Artikel online veröffentlicht:
04. Juli 2022

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