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DOI: 10.1055/s-0042-1746415
Antibacterial and Cytotoxicity of Root Canal Sealer with the Addition of Chitosan Nanoparticle at Various Concentrations
Funding This study was funded by Faculty of Dentistry, Universitas Gadjah Mada (grant 656/UN1/FKG1/Set.KG1/LT/2020).
Abstract
Objectives The aim of this study was to evaluate the addition of chitosan nanoparticle with concentrations of 0, 10, 20, and 30% to the epoxy resin-based (ERB) sealer on its antibacterial and cytotoxicity effect.
Methods and Materials This research was divided into two studies, the first study was the addition of chitosan with a concentration of 0% (as control), 10, 20, and 30% to an ERB sealer on its antibacterial effect, and the second study was on its cytotoxicity. An agar diffusion test was employed to determine the antibacterial effect on Enterococcus faecalis. An MTT (3-{4,5-dimethylthiazol-2-yl}-2,5-diphenyl tetrazolium bromide) assay was utilized to test the cytotoxicity by evaluating cell viability.
Statistical Analysis One-way analysis of variance and Tukey's test (α = 0.05) were used to analyze data obtained from each evaluation with a significance level of 95%.
Results The addition of chitosan nanoparticles at concentrations 10, 20, and 30% produced a greater inhibition zone of E. faecalis (p < 0.05), however, had less cytotoxicity compared with no addition of chitosan (0%) (p < 0.05).
Conclusion The addition of chitosan nanoparticles at concentrations 10, 20, and 30% to the ERB sealer produced greater antibacterial and less cytotoxicity compared with no addition of chitosan (0%).
Publikationsverlauf
Artikel online veröffentlicht:
21. Juni 2022
© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)
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References
- 1 Chandra SS, Shankar P, Indira R. Depth of penetration of four resin sealers into radicular dentinal tubules: a confocal microscopic study. J Endod 2012; 38 (10) 1412-1416
- 2 Dalmia S, Gaikwad A, Samuel R, Aher G, Gulve M, Kolhe S. Antimicrobial efficacy of different endodontic sealers against Enterococcus faecalis: an in vitro study. J Int Soc Prev Community Dent 2018; 8 (02) 104-109
- 3 Torres-Rosas R, Torres-Gómez N, Moreno-Rodríguez A, García-Contreras R, Argueta-Figueroa L. Anti-Inflammatory and antibacterial activity of the chitosan/chlorhexidine gel commercial preparation for postexodontia treatment: an in vitro study. Eur J Dent 2020; 14 (03) 397-403
- 4 Kaur A, Shah N, Logani A, Mishra N. Biotoxicity of commonly used root canal sealers: a meta-analysis. J Conserv Dent 2015; 18 (02) 83-88
- 5 Fonseca DA, Paula AB, Marto CM. et al. Biocompatibility of root canal sealers: a systematic review of in vitro and in vivo studies. Materials (Basel) 2019; 12 (24) 1-34
- 6 Huang Y, Li X, Mandal P. et al. The in vitro antimicrobial activities of four endodontic sealers. BMC Oral Health 2019; 19 (01) 118-125
- 7 AL-Haddad A, Che Ab Aziz ZA. Bioceramic-based root canal sealers: a review. Int J Biomater 2016; 2016: 9753210
- 8 Devadiga SB, Shetty D, Lakhsmi G, Packiyam JE. Synthesis of chitosan silver nanoparticles from chitin of crustacean shell and its applications. Int J Curr Res Chem Pharmac Sci 2016; 3 (05) 1-5
- 9 Del Carpio-Perochena A, Kishen A, Shrestha A, Bramante CM. Antibacterial properties associated with chitosan nanoparticle treatment on root dentin and 2 types of endodontic sealers. J Endod 2015; 41 (08) 1353-1358
- 10 Aydın ZU, Özyürek T, Keskin B, Baran T. Effect of chitosan nanoparticle, QMix, and EDTA on TotalFill BC sealers' dentinal tubule penetration: a confocal laser scanning microscopy study. Odontology 2019; 107 (01) 64-71
- 11 Enggardipta RA, Untara RTE, Santosa P, Kartikaningtyas AT, Widyastuti A, Ratih DN. Apical sealing ability of chitosan nanoparticles in epoxy-resin-based endodontic sealer. MKGI 2019; 5 (02) 69-74
- 12 Ratih DN, Enggardipta RA, Kusumo ANH, Hadriyanto W. Setting time, flowability, and solubility of epoxy resin-based sealer mixed with chitosan nanoparticles. Int J Appl 2021; 13 (02) 122-126
- 13 Putranto AW, Suprastiwi E, Meidyawati R, Agusnar H. Characterization of novel cement-based carboxymethyl chitosan/amorphous calcium phosphate. Eur J Dent 2022; 16 (04) 809-814
- 14 Silva AR, Santos EB, Pinto SCS, Gomes JC, Vaz IP, Carvalho MF. Antimicrobial effect and transdentinal diffusion of new intracanal formulations containing nitrofurantoin or doxycycline. Braz Dent J 2014; 25 (05) 425-429
- 15 Levinson W. Review of Medical Microbiology and Immunology. 9th ed.. New York: McGraw Hill; 2006: 278-300
- 16 International Organization For Standardization. Biological evaluation of medical devices - Part 12: Sample preparation and reference materials. Geneva: ISO 10993–12; 2012
- 17 Catunda RQ, Vieira JRC, de Oliveira EB, da Silva EC, Brasil VLM, Perez DC. Cytotoxicity evaluation of three dental adhesives on Vero cells in vitro . J Clin Exp Dent 2017; 9 (01) e61-e66
- 18 Badole GP, Warhadpande MM, Meshram GK. et al. A comparative evaluation of cytotoxicity of root canal sealers: an in vitro study. Restor Dent Endod 2013; 38 (04) 204-209
- 19 Ahrari F, Tavakkol Afshari J, Poosti M, Brook A. Cytotoxicity of orthodontic bonding adhesive resins on human oral fibroblasts. Eur J Orthod 2010; 32 (06) 688-692
- 20 Monajemzadeh A, Ahmadi Asoor S, Aslani S, Sadeghi-Nejad B. In vitro antimicrobial effect of different root canal sealers against oral pathogens. Curr Med Mycol 2017; 3 (02) 7-12
- 21 Gjorgievska ES, Nicholson JW, Coleman NJ, Booth S, Dimkov A, Hurt A. Component release and mechanical properties of endodontic sealers following incorporation of antimicrobial agents. BioMed Res Int 2017; 2017: 2129807
- 22 Nair N, James B, Devadathan A, Johny MK, Mathew J, Jacob J. Comparative evaluation of antibiofilm efficacy of chitosan nanoparticle and zinc oxide nanoparticle-incorporated calcium hydroxide-based sealer: an in vitro study. Contemp Clin Dent 2018; 9 (03) 434-439
- 23 Soliman AY, Roshdy NB, Lutfy RA. Assessment of the effect of chitosan nanoparticles on the ultra-structure of dentinal wall: a comparative in vitro study. Acta Sci Dent Sci 2020; 4 (01) 32-38
- 24 Poggio C, Trovati F, Ceci M, Colombo M, Pietrocola G. Antibacterial activity of different root canal sealers against Enterococcus faecalis . J Clin Exp Dent 2017; 9 (06) e743-e748
- 25 Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal 2016; 6 (02) 71-79
- 26 Matalon S, Weiss EI, Gorfil C, Noy D, Slutzky H. In vitro antibacterial evaluation of flowable restorative materials. Quintessence Int 2009; 40 (04) 327-332
- 27 Teixeira L, Basso FG, Hebling J. et al. Cytotoxicity evaluation of root canal sealers using an in vitro experimental model with roots. Braz Dent J 2017; 28 (02) 165-171
- 28 Jung S, Sielker S, Hanisch MR, Libricht V, Schäfer E, Dammaschke T. Cytotoxic effects of four different root canal sealers on human osteoblasts. PLoS One 2018; 13 (03) e0194467
- 29 Graunaite I, Lodiene G, Arandarcikaite O, Pukalskas A, Machiulskiene V. Leachables and cytotoxicity of root canal sealers. J Oral Sci 2018; 60 (03) 381-387
- 30 Sajnani AR, Hegde MN. Leaching of monomers from bulk-fill composites: an in vitro study. J Conserv Dent 2016; 19 (05) 482-486