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CC BY 4.0 · Eur J Dent 2023; 17(03): 790-796
DOI: 10.1055/s-0042-1755550
Original Article

Novel Application of 3D Scaffolds of Poly(E-Caprolactone)/Graphene as Osteoinductive Properties in Bone Defect

Hendrik Setia Budi 
1   Department of Oral Biology, Dental Pharmacology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Silvia Anitasari 
2   Department of Dental Material and Devices, Dentistry Program, Faculty of Medicine, Universitas Mulawarman, Samarinda, Indonesia
3   Department Medical Microbiology, Medical Program, Faculty of Medicine, Universitas Mulawarman, Samarinda, Indonesia
,
Yung-Kang Shen
4   School of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan
,
Marut Tangwattanachuleeporn
5   Faculty of Allied Health Sciences, Burapha University, Chon Buri, Thailand
6   Research Unit for Sensor Innovation, Burapha University, Chon Buri, Thailand
,
Prawati Nuraini
7   Department of Pediatric Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Narendra Arya Setiabudi
8   Medical Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
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Abstract

Objective Scaffolds provided a surface on which cells could attach, proliferate, and differentiate. Nowadays, bone tissue engineering offers hope for treating bone cancer. Poly(e-caprolactone) (PCL)/graphene have capability as an osteogenic and regenerative therapy. It could be used to produce bone tissue engineering scaffolds. The purpose of this study was to investigate the ability of PCL/graphene to enhance the osteoinductive mechanism.

Materials and Methods The PCL/graphene scaffold was developed utilizing a particulate-leaching process and cultured with osteoblast-like cells MG63 at 0.5, 1.5, and 2.5 wt% of graphene. We evaluated the porosity, pore size, migratory cells, and cell attachment of the scaffold.

Statistical Analysis Data was expressed as the mean ± standard error of the mean and statistical analyses were performed using one-way analysis of variance and Tukey's post hoc at a level of p-value < 0.05.

Results Porosity of scaffold with various percentage of graphene was nonsignificant (p > 0.05). There were differences in the acceleration of cell migration following wound closure between groups at 24 hours (p < 0.01) and 48 hours (p < 0.00). Adding the graphene on the scaffolds enhanced migration of osteoblast cells culture and possibility to attach. Graphene on 2.5 wt% exhibited good characteristics over other concentrations.

Conclusion This finding suggests that PCL/graphene composites may have potential applications in bone tissue engineering.

Keywords

PCL - graphene - scaffold - osteoinductive - tissue engineering

Authors' Contributions

H.S.B. reports all support for study design, collection of data, data analysis/interpretation, writing of the manuscript, and revision of the manuscript.


S.A. reports all support for data analysis/interpretation, writing of the manuscript, and revision of the manuscript.


N.A.S. reports support for collection of data and writing of the manuscript.


Y.-K.S. reports all support for study design, writing of the manuscript, and revision of the manuscript.


M.T. reports all support for data analysis/interpretation and writing of the manuscript.




Publication History

Article published online:
09 November 2022

© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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