Download PDF
CC BY 4.0 · Eur J Dent 2023; 17(03): 823-827
DOI: 10.1055/s-0042-1755616
Original Article

IgA Levels among Type 2 Diabetic and Non-Diabetic Patients with Periodontitis: A Prospective Clinical Study

Pooja Palwankar
1   Department of Periodontology, Manav Rachna Dental College FDS, MRIIRS Faridabad, Haryana, India
,
Saumya Jain
1   Department of Periodontology, Manav Rachna Dental College FDS, MRIIRS Faridabad, Haryana, India
,
Ruchi Pandey
1   Department of Periodontology, Manav Rachna Dental College FDS, MRIIRS Faridabad, Haryana, India
,
Shakila Mahesh
2   Department of Microbiology, Manav Rachna Dental College FDS, MRIIRS Faridabad, Haryana, India
› Author Affiliations Funding None.
› Further Information
Also available at
   Permissions and Reprints

Abstract

Objectives To estimate gingival crevicular immunoglobulin A(IgA) using enzyme-linked immunosorbent assay (ELISA) among type II diabetic patients with periodontitis.

Materials and Methods A non-randomized study was done of 40 periodontitis subjects with a mean age of 50 years and were recruited into two groups, Group A (Type II controlled diabetics with HbA1c < 7%) and Group B (non-diabetics with HbA1c between 4 and 6%). Both the groups underwent nonsurgical periodontal therapy (NSPT). The clinical parameters were recorded at baseline, 1, and 3 months. GCF sample was collected for the estimation of crevicular IgA at baseline and at 3 months.

Statistical Analysis Results were analyzed using parametric tests paired t-test and Student's t-test for every assessment point. The level of significance was set at p < 0.05.

Results Difference in IgA levels and clinical parameters was seen between diabetic and non-diabetic groups, which was statistically significant.

Conclusion Changes in crevicular IgA levels in patients with diabetic periodontitis can be used as a novel biomarker in assessing the inflammatory status.

Keywords

Enzyme linked immunosorbent assay - Immunoglobulin A - nonsurgical periodontal therapy - gingival crevicular fluid - diabetes mellitus - diabetes biomarkers


Publication History

Article published online:
27 September 2022

© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India

 

  • References

  • 1 Palwankar P, Gupta K, Goyal A, Gopal L. Photodynamic therapy and its effect on oxidative stress following nonsurgical therapy in subjects with periodontitis: a clinico-biochemical study. IJDSIR 2020; 3: 77-84
    Google Scholar
  • 2 Pranam S, Palwankar P, Pandey R, Goyal A. Evaluation of efficacy ofcoenzyme Q10 as an adjunct to nonsurgical periodontal therapy and its effect on crevicular superoxide dismutase in patients with chronic periodontitis. Eur J Dent 2020; 14 (04) 551-557
    Article in Thieme ConnectPubMedGoogle Scholar
  • 3 Agrali OB, Kuru BE. Periodontal treatment in a generalized severe chronic periodontitis patient: a case report with 7-year follow-up. Eur J Dent 2015; 9 (02) 288-292
    Article in Thieme ConnectPubMedGoogle Scholar
  • 4 Bakema JE, van Egmond M. Immunoglobulin A: a next generation of therapeutic antibodies?. MAbs 2011; 3 (04) 352-361
    CrossrefPubMedGoogle Scholar
  • 5 Gupta G. Gingival crevicular fluid as a periodontal diagnostic indicator- II: Inflammatory mediators, host-response modifiers and chair side diagnostic aids. J Med Life 2013; 6 (01) 7-13
    PubMedGoogle Scholar
  • 6 Heji ES, Bukhari AA, Bahammam MA, Homeida LA, Aboalshamat KT, Aldahlawi SA. Periodontal disease as a predictor of undiagnosed diabetes or prediabetes in dental patients. Eur J Dent 2021; 15 (02) 216-221
    Article in Thieme ConnectPubMedGoogle Scholar
  • 7 Gupta N, Gupta ND, Gupta A, Goyal L, Garg S. The influence of type 2 diabetes mellitus on salivary matrix metalloproteinase-8 levels and periodontal parameters: a study in an Indian population. Eur J Dent 2015; 9 (03) 319-323
    Article in Thieme ConnectPubMedGoogle Scholar
  • 8 Engvall E. The ELISA, enzyme-linked immunosorbent assay. Clin Chem 2010; 56 (02) 319-320
    CrossrefPubMedGoogle Scholar
  • 9 Breedveld A, van Egmond M. IgA and FcαRI: pathological roles and therapeutic opportunities. Front Immunol 2019; 10: 553
    CrossrefPubMedGoogle Scholar
  • 10 Joseph R, Sasikumar M, Mammen J, Joseraj MG, Radhakrishnan C. Nonsurgical periodontal-therapy improves glycosylated hemoglobin levels in pre-diabetic patients with chronic periodontitis. World J Diabetes 2017; 8 (05) 213-221
    CrossrefPubMedGoogle Scholar
  • 11 Ahuja CR, Kolte AP, Kolte RA, Gupta M, Chari S. Effect of non-surgical periodontal treatment on gingival crevicular fluid and serum leptin levels in periodontally healthy chronic periodontitis and periodontitis subjects with type 2 diabetes mellitus. J Investig Clin Dent 2019; 10: e12420
    CrossrefPubMedGoogle Scholar
  • 12 Dodwad V, Ahuja S, Kukreja BJ. Effect of locally delivered tetracycline hydrochloride as an adjunct to scaling and root planing on Hba1c, C-reactive protein, and lipid profile in type 2 diabetes: a clinico-biochemical study. Contemp Clin Dent 2012; 3 (02) 150-154
    CrossrefPubMedGoogle Scholar
  • 13 Perayil J, Suresh N, Fenol A, Vyloppillil R, Bhaskar A, Menon S. Comparison of glycated hemoglobin levels in individuals without diabetes and with and without periodontitis before and after non-surgical periodontal therapy. J Periodontol 2014; 85 (12) 1658-1666
    CrossrefPubMedGoogle Scholar
  • 14 Hungund S, Panseriya BJ. Reduction in HbA1c levels following non-surgical periodontal therapy in type-2 diabetic patients with chronic generalized periodontitis: a periodontist's role. J Indian Soc Periodontol 2012; 16 (01) 16-2114
    CrossrefPubMedGoogle Scholar
  • 15 Palwankar P, Rana M, Arora K, Deepthy C. Evaluation of non- surgical therapy on glutathione levels in chronic periodontitis. Eur J Dent 2015; 9 (03) 415-422
    Article in Thieme ConnectPubMedGoogle Scholar
  • 16 Agarwal MC, Chaubey KK, Madan E, Agarwal S. Effect of periodontal therapy on type 2 diabetes mellitus patients with chronic periodontitis with the evaluation of HbA1c. J Int Clin Dent Res Organ 2016; 8 (01) 34-38
    CrossrefGoogle Scholar
  • 17 Moeintaghavi A, Arab HR, Bozorgnia Y, Kianoush K, Alizadeh M. Non-surgical periodontal therapy affects metabolic control in diabetics: a randomized controlled clinical trial. Aust Dent J 2012; 57 (01) 31-37
    CrossrefPubMedGoogle Scholar
  • 18 Kudva P, Tabasum ST, Garg N. Evaluation of clinical and metabolic changes after non surgical periodontal treatment of type 2 diabetes mellitus patients: a clinico biochemical study. J Indian Soc Periodontol 2010; 14 (04) 257-262
    CrossrefPubMedGoogle Scholar
  • 19 Butchibabu K, Swaminathan M, Kumar S, Koppolu P, Kiran K, Muralikrishna T. Estimation of salivary immunoglobulin A levels in gingivitis and chronic periodontitis subjects before and after phase I periodontal therapy. J NTR Univ Health Sci 2014; 3 (05) 23-27
    CrossrefGoogle Scholar
  • 20 Awartani F. Serum immunoglobulin levels in type 2 diabetes patients with chronic periodontitis. J Contemp Dent Pract 2010; 11 (03) 1-10
    CrossrefGoogle Scholar