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CC BY-NC-ND 4.0 · Ibnosina Journal of Medicine and Biomedical Sciences 2022; 14(03): 116-126
DOI: 10.1055/s-0042-1756684
Original Article

Antioxidant and Anti-Anemic Effects of Ethanol Leaf Extracts of Mucuna poggei and Telfairia occidentalis in Phenyl-Hydrazine-Induced Anemia in Wistar Albino Rats

Precious A. Udeozor
1   Department of Biochemistry, College of Science, Evangel University, Akaeze, Ebonyi State, Nigeria
2   Department of Biochemistry, Faculty of Science, Ebonyi State University, P.M.B. 053 Abakaliki, Ebonyi State, Nigeria
,
Udu A. Ibiam
1   Department of Biochemistry, College of Science, Evangel University, Akaeze, Ebonyi State, Nigeria
2   Department of Biochemistry, Faculty of Science, Ebonyi State University, P.M.B. 053 Abakaliki, Ebonyi State, Nigeria
,
Daniel E. Uti
4   Department of Biochemistry, College of Medicine, Federal University of Health Sciences, Otukpo, Benue State, Nigeria
,
Grace U. Umoru
1   Department of Biochemistry, College of Science, Evangel University, Akaeze, Ebonyi State, Nigeria
,
Eucharia N. Onwe
3   Department of Biotechnology, College of Science, Evangel University, Akaeze, Ebonyi State, Nigeria
,
Francis O. Mbonu
9   Department of Science Laboratory Technology, Akanu Ibiam Federal Polytechnic, Unwana, Afikpo, Ebonyi State, Nigeria
,
Wilson A. Omang
6   Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, Calabar, Nigeria
,
Samuel I. Ijoganu
5   Department of Human Physiology,, College of Medicine, Federal University of Health Sciences, Otukpo, Benue State, Nigeria
,
Charles O. Anaga
1   Department of Biochemistry, College of Science, Evangel University, Akaeze, Ebonyi State, Nigeria
,
Jenavine Onyinye Mbah
7   Department of Science Laboratory Technology, Faculty of Biological Sciences, University of Calabar, Calabar, Nigeria
,
Solomon K. Nwadum
8   Department. of Pharmacology and Therapeutics, Faculty of Clinical Basic Medicine, Ebonyi State University Abakaliki, Nigeria
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Abstract

Introduction With the use of recent therapeutic data, several methods for finding medications from untapped natural materials continue to advance research and development. The antioxidant and antianemic effects of ethanol leaf extracts of Telfairia occidentalis and Mucuna poggei in phenylhydrazine-induced anemia in Wister albino rats were investigated.

Method A total of 54 male albino rats were randomly assigned to nine experimental groups (n = 6). Anemia was induced in groups 2 to 9 with 10 mg/kg body weight of phenylhydrazine. Group 1 (normal control) rats were administered normal saline only. Group 2 (anemic control) rats were untreated. Group 3 (standard controls) rats were treated with a standard multivitamin, groups 4, 5, and 6 rats were treated with varying doses of M. poggei leaf extract respectively, while groups 7, 8, and 9 rats were treated with similar doses of T. occidentalis leaf extract by oral intubation for 21 days.

Results Earlier, the gas chromatography-mass spectrometry analysis indicated eighteen (18) and twenty-nine (29) active compounds present in M. poggei and T. occidentalis, respectively. More so, T. occidentalis and M. poggei were studied for their inhibitory potentials against 2,2-diphenyl-1 picrylhydrazyl-free radicals, nitric oxide, phosphomolybdenum, and ferric-reducing antioxidant power. M. poggei and T. occidentalis also showed the following trend of minerals Mg > Ca > Fe > Na > Zn > Mn > K and Mg > Fe > Ca > Na > K > Mn > Zn, respectively. The results also showed that the packed cell volume, red blood cells, and hemoglobin significantly (p < 0.05) decreased in anemic control rats compared with normal controls and were significantly (p < 0.05) increased on treatment with the leaf extracts. Catalase, superoxide dismutase, glutathione peroxidase activities, and glutathione levels significantly (p < 0.05) decreased in anemic control, compared with normal. However, these significantly (p < 0.05) increased on treatment with the leaf extracts in a dose-independent manner. Malondialdehyde levels took the reverse trend.

Conclusion This study indicated that leaf extracts of M. poggei and T. occidentalis can be used in the management of anemia and oxidative stress-related complications

Keywords

Mucuna poggei - Telfairia occidentalis - phenylhydrazine - antioxidant - oxidative stress

Authors' Contributions

UAI and PAU conceived and designed the study. GUU, PAU UNO, JOM, ISI, and FOM performed literature research, COA, DEU, SKN, and PAU performed the laboratory analysis. PAU wrote the manuscript. UAI and DEU read, edited, and revised the manuscript for intellectual content. All authors have read and approved the final manuscript.


Compliance with Ethical Principles

This study was in compliance with the Convention on Trade in Endangered Species of Wild Fauna and Flora. Furthermore, the study was approved by the Department of Biochemistry Ethical Committee on Research, Innovation and Institutional Ethical Committee of Ebonyi State University Abakaliki, Nigeria.




Publication History

Article published online:
21 December 2022

© 2022. The Libyan Authority of Scientific Research and Technology and the Libyan Biotechnology Research Center. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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