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CC BY 4.0 · Eur J Dent 2023; 17(03): 623-628
DOI: 10.1055/s-0042-1757468
Review Article

Micro Computed Tomography and Immunohistochemistry Analysis of Dental Implant Osseointegration in Animal Experimental Model: A Scoping Review

Annisa Putri
1   Dentomaxillofacial Radiology Residency Program, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia
,
Farina Pramanik
2   Department of Dentomaxillofacial Radiology, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia
,
Azhari Azhari
2   Department of Dentomaxillofacial Radiology, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia
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Abstract

Osseointegration is a complex process that involves the interaction of dental implants, bone, and the immune system. Preclinical testing was carried out to develop a better understanding of the mechanism. Micro-computed tomography (micro-CT) imaging techniques and immunohistochemistry are excellent tools for this objective as both enable quantitative assessment of bone microarchitecture and intercellular interaction. An extensive literature search was conducted using the databases PubMed, Science Direct, Wiley Online, Proquest and Ebscohost from January 2011 to January 2021. Among the publications retrieved, the rat model was the most frequently used experimental protocol, with the tibia being the most frequently implanted site. The region of interest demonstrates a high degree of homogeneity as measured by trabecula but varies in size and shape. The most frequently mentioned micro-CT bone parameter and immunohistochemistry bone markers were bone volume per total volume (BV/TV) and runt-related transcription factors (RUNX). Animal models, micro-CT analysis methods, and immunohistochemistry biomarkers yielded a variety of results in the studies. Understanding bone architecture and the remodeling process will aid in the selection of a viable model for a specific research topic.

Keywords

dental implant - osseointegration - animal model - micro-CT - immunohistochemistry

Authors' Contributions

A.A. conceptualized the manuscript and supervision; F.P. and A.P. were involved in the methodology, writing, review, and editing of the manuscript; all authors read and agreed to the published version of the manuscript.




Publication History

Article published online:
28 March 2023

© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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