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CC BY 4.0 · Eur J Dent 2023; 17(04): 1120-1128
DOI: 10.1055/s-0042-1758789
Original Article

Influence of Lithium- and Zinc-Containing Bioactive Glasses on Pulpal Regeneration

An Thi Loc Tran
1   Dental Biomaterials Science Program, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Charnsak Sukajintanakarn
2   Department of Conservative Dentistry and Prosthodontics, Faculty of Dentistry, Srinakharinwirot University, Bangkok, Thailand
,
Pisol Senawongse
3   Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Hathaitip Sritanaudomchai
4   Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Nisarat Ruangsawasdi
5   Department of Pharmacology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Puangwan Lapthanasupkul
6   Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Nakarin Kitkumthorn
4   Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Naruporn Monmaturapoj
7   Assistive Technology and Medical Devices Research Center, National Science and Technology Development Agency, Pathum Thani, Thailand
,
Chutikarn Khamsut
7   Assistive Technology and Medical Devices Research Center, National Science and Technology Development Agency, Pathum Thani, Thailand
,
Parichart Naruphontjirakul
8   Biological Engineering Program, Faculty of Engineering, King Mongkut's University of Technology Thonburi, Bangkok, Thailand
,
Pong Pongprueksa 
3   Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
› Institutsangaben
Funding Dr. An Thi Loc Tran has been granted a PhD fellowship from the International Dental Collaboration of the Mekong River Region (IDCMR), Faculty of Dentistry, Mahidol University. This research was partially supported by the National Research Council of Thailand (NRCT) and Mahidol University (Project Number: NRCT5-RSA63015-15).
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Abstract

Objective To evaluate the potential of modified bioactive glasses containing lithium and zinc as pulp capping materials by investigating the odontogenic differentiation and mineralization response in the tooth culture model.

Materials and Methods Lithium- and zinc-containing bioactive glasses (45S5.1Li, 45S5.5Li, 45S5.1Zn, 45S5.5Zn, 45S5.1Zn sol-gel, and 45S5.5Zn sol-gel), fibrinogen-thrombin, and biodentine were prepared to assess Axin2 gene expression at 0, 30 minutes, 1 hour, 12 hours, and 1 day and DSPP gene expression at 0, 3, 7, and 14 days in stem cells from human exfoliated deciduous teeth (SHEDs) using qRT-PCR. The experimental bioactive glasses incorporated with fibrinogen-thrombin and biodentine were placed on the pulpal tissue in the tooth culture model. Histology and immunohistochemistry were analyzed at 2 weeks and 4 weeks.

Results Axin2 gene expression for all experimental groups was significantly higher than the control at 12 hours. The DSPP gene expression for all experimental groups was significantly higher than the control at 14 days. The presence of mineralization foci was significantly higher at 4 weeks for the modified bioactive glasses 45S5.5Zn, 45S5.1Zn sol-gel, and 45S5.5Zn sol-gel as well as Biodentine compared with the fibrinogen-thrombin control.

Conclusion Lithium- and zinc-containing bioactive glasses increased Axin2 and DSPP gene expression in SHEDs and can potentially enhance pulp mineralization and regeneration. Zinc-containing bioactive glasses are a promising candidate to be used as pulp capping materials.

Keywords

bioactive glass - lithium bioactive glass - zinc bioactive glass - tooth culture model - fibrinogen thrombin


Publikationsverlauf

Artikel online veröffentlicht:
22. Februar 2023

© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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