PDF herunterladen
CC BY 4.0 · Eur J Dent 2023; 17(04): 1189-1193
DOI: 10.1055/s-0042-1758794
Original Article

Dental Implant Osseointegration Inhibition by Nicotine through Increasing nAChR, NFATc1 Expression, Osteoclast Numbers, and Decreasing Osteoblast Numbers

Nina Nilawati
1   Department of Periodontology, Faculty of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
2   Department of Periodontology, Haji General Hospital, Surabaya, Indonesia
,
Widyastuti Widyastuti
1   Department of Periodontology, Faculty of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
,
Yoifah Rizka
1   Department of Periodontology, Faculty of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
,
Hansen Kurniawan 
1   Department of Periodontology, Faculty of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
› Institutsangaben
› Weitere Informationen
Auch verfügbar auf
   Lizenzen und Reprints

Abstract

Objective The success of dental implants is determined by the osteointegration process. Many studies state that smoking cigarettes can inhibit osseointegration, but the inhibition mechanism is still unclear.

The aim of this study was to identify and analyze the effect of nicotine on the inhibition of dental implant osseointegration through the expression of nicotinic acetylcholine receptor (nAChR), nuclear factor of activated T cells cytoplasmic 1 (NFATc1), osteoclast, and osteoblast numbers.

Materials and Methods This study is an experimental study of 16 New Zealand rabbits, randomized across two groups. Group 1 (eight rabbits) was a control group, and group 2 (eight rabbits) was a treatment group. The treatment group was given 2.5 mg/kg body weight/day of nicotine by injection 1 week before placement of the implant until the end of research. Observations were made in the first and the eighth week by measuring the number of osteoblast and osteoclast by immunohistology test and the expression of nAChR and NFATc1 by immunohistochemistry test.

Statistical Analysis Data was analyzed using a one-way analysis of variance and Student's t-test. A p-value of < 0.05 was considered statistically significant.

Results Significant differences were found between the control and treatment groups (p < 0.05). Results showed that nicotine increases the expression of nAChR and decreases the number of osteoblasts and the expression of BMP2 and osteocalcin.

Conclusion Nicotine inhibits the osseointegration of dental implants by increasing nAChR, NFATc1, osteoclast numbers, and decreasing osteoblast numbers.

Keywords

dental implant - nicotine - nAChR - NFATc1 - osteoclast - osteoblast - osseointegration


Publikationsverlauf

Artikel online veröffentlicht:
27. Dezember 2022

© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India

 

  • References

  • 1 Shenava S, Singh P, Sharath Babu C, Kumar V, Jyoti B, Sharma S. Co-relation between smoking and bone healing around dental implants: a clinical study. J Int Oral Health 2016; 8: 1-3
    CrossrefGoogle Scholar
  • 2 Arora A, Reddy MM, Mhatre S, Bajaj A, Gopinath PV, Arvind P. Comparative evaluation of effect of smoking on survival of dental implant. J Int Oral Health 2017; 9: 24-27
    CrossrefGoogle Scholar
  • 3 Schwartz-Arad D, Samet N, Samet N, Mamlider A. Smoking and complications of endosseous dental implants. J Periodontol 2002; 73 (02) 153-157
    CrossrefPubMedGoogle Scholar
  • 4 Heitz-Mayfield LJA, Huynh-Ba G. History of treated periodontitis and smoking as risks for implant therapy. Int J Oral Maxillofac Implants 2009; 24 (Suppl): 39-68
    Google Scholar
  • 5 Cavalcanti R, Oreglia F, Manfredonia MF, Gianserra R, Esposito M. The influence of smoking on the survival of dental implants: a 5-year pragmatic multicentre retrospective cohort study of 1727 patients. Eur J Oral Implantology 2011; 4 (01) 39-45
    PubMedGoogle Scholar
  • 6 Chrcanovic BR, Albrektsson T, Wennerberg A. Smoking and dental implants: a systematic review and meta-analysis. J Dent 2015; 43 (05) 487-498
    CrossrefPubMedGoogle Scholar
  • 7 Tymkiw KD, Thunell DH, Johnson GK. et al. Influence of smoking on gingival crevicular fluid cytokines in severe chronic periodontitis. J Clin Periodontol 2011; 38 (03) 219-228
    CrossrefPubMedGoogle Scholar
  • 8 García B, Penarrocha M, Martí E, Gay-Escodad C, von Arx T. Pain and swelling after periapical surgery related to oral hygiene and smoking. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2007; 104 (02) 271-276
    CrossrefPubMedGoogle Scholar
  • 9 Larrazábal C, García B, Peñarrocha M, Peñarrocha M. Influence of oral hygiene and smoking on pain and swelling after surgical extraction of impacted mandibular third molars. J Oral Maxillofac Surg 2010; 68 (01) 43-46
    CrossrefPubMedGoogle Scholar
  • 10 Shibli JA, Piattelli A, Iezzi G. et al. Effect of smoking on early bone healing around oxidized surfaces: a prospective, controlled study in human jaws. J Periodontol 2010; 81 (04) 575-583
    CrossrefPubMedGoogle Scholar
  • 11 Bezerra Ferreira JD, Rodrigues JA, Piattelli A, Iezzi G, Gehrke SA, Shibli JA. The effect of cigarette smoking on early osseointegration of dental implants: a prospective controlled study. Clin Oral Implants Res 2016; 27 (09) 1123-1128
    CrossrefPubMedGoogle Scholar
  • 12 Chrcanovic BR, Kisch J, Albrektsson T, Wennerberg A. Factors influencing early dental implant failures. J Dent Res 2016; 95 (09) 995-1002
    CrossrefPubMedGoogle Scholar
  • 13 Nazeer J, Singh R, Suri P. et al. Evaluation of marginal bone loss around dental implants in cigarette smokers and nonsmokers. A comparative study. J Family Med Prim Care 2020; 9 (02) 729-734
    CrossrefPubMedGoogle Scholar
  • 14 Takamiya AS, Goiato MC, Gennari Filho H. Effect of smoking on the survival of dental implants. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2014; 158 (04) 650-653
    CrossrefPubMedGoogle Scholar
  • 15 Ghayor C, Correro RM, Lange K, Karfeld-Sulzer LS, Grätz KW, Weber FE. Inhibition of osteoclast differentiation and bone resorption by N-methylpyrrolidone. J Biol Chem 2011; 286 (27) 24458-24466
    CrossrefPubMedGoogle Scholar
  • 16 Choi J, Choi SY, Lee SY. et al. Caffeine enhances osteoclast differentiation and maturation through p38 MAP kinase/Mitf and DC-STAMP/CtsK and TRAP pathway. Cell Signal 2013; 25 (05) 1222-1227
    CrossrefPubMedGoogle Scholar
  • 17 Kwak HB, Yang D, Ha H. et al. Tanshinone IIA inhibits osteoclast differentiation through down-regulation of c-Fos and NFATc1. Exp Mol Med 2006; 38 (03) 256-264
    CrossrefPubMedGoogle Scholar
  • 18 Takayanagi H, Kim S, Koga T. et al. Induction and activation of the transcription factor NFATc1 (NFAT2) integrate RANKL signaling in terminal differentiation of osteoclasts. Dev Cell 2002; 3 (06) 889-901
    CrossrefPubMedGoogle Scholar
  • 19 En-Nosse M, Hartmann S, Trinkaus K. et al. Expression of non-neuronal cholinergic system in osteoblast-like cells and its involvement in osteogenesis. Cell Tissue Res 2009; 338 (02) 203-215
    CrossrefPubMedGoogle Scholar
  • 20 Liang D, Wang KJ, Tang ZQ. et al. Effects of nicotine on the metabolism and gene expression profile of Sprague‑Dawley rat primary osteoblasts. Mol Med Rep 2018; 17 (06) 8269-8281
    PubMedGoogle Scholar
  • 21 Søballe K. Hydroxyapatite ceramic coating for bone implant fixation. Mechanical and histological studies in dogs. Acta Orthop Scand Suppl 1993; 255: 1-58
    CrossrefPubMedGoogle Scholar
  • 22 Shroff Y, Shah M, Vyas M, Pandya R. Smoking and implant failure: an evidence based review. IOSR J Dent Med Sci 2018; 17: 60-64
    Google Scholar
  • 23 Pereira ML, Carvalho JC, Peres F, Fernandes MH. Effect of nicotine in matrix mineralization by human bone marrow and Saos-2 cells cultured on the surface of plasma-sprayed titanium implants. J Biomed Mater Res A 2009; 88 (01) 84-93
    CrossrefPubMedGoogle Scholar
  • 24 Kim BS, Kim SJ, Kim HJ. et al. Effects of nicotine on proliferation and osteoblast differentiation in human alveolar bone marrow-derived mesenchymal stem cells. Life Sci 2012; 90 (3−4): 109-115
    CrossrefPubMedGoogle Scholar
  • 25 Nitzan D, Mamlider A, Levin L, Schwartz-Arad D. Impact of smoking on marginal bone loss. Int J Oral Maxillofac Implants 2005; 20 (04) 605-609
    Google Scholar
  • 26 Garg A. Pathophysiology of tobacco use and wound healing. Dent Implantol Update 2010; 21 (01) 1-4
    PubMedGoogle Scholar
  • 27 Henemyre CL, Scales DK, Hokett SD. et al. Nicotine stimulates osteoclast resorption in a porcine marrow cell model. J Periodontol 2003; 74 (10) 1440-1446
    CrossrefPubMedGoogle Scholar
  • 28 Adler ID, Attia SM. Nicotine is not clastogenic at doses of 1 or 2 mg/kg body weight given orally to male mice. Mutat Res 2003; 542 (1-2): 139-142
    CrossrefPubMedGoogle Scholar
  • 29 Tanaka H, Tanabe N, Shoji M. et al. Nicotine and lipopolysaccharide stimulate the formation of osteoclast-like cells by increasing macrophage colony-stimulating factor and prostaglandin E2 production by osteoblasts. Life Sci 2006; 78 (15) 1733-1740
    CrossrefPubMedGoogle Scholar
  • 30 Kliemann K, Kneffel M, Bergen I. et al. Quantitative analyses of bone composition in acetylcholine receptor M3R and alpha7 knockout mice. Life Sci 2012; 91 (21−22): 997-1002
    CrossrefPubMedGoogle Scholar
  • 31 Tanaka H, Tanabe N, Kawato T. et al. Nicotine affects bone resorption and suppresses the expression of cathepsin K, MMP−9 and vacuolar-type H(+)-ATPase d2 and actin organization in osteoclasts. PLoS One 2013; 8 (03) e59402
    CrossrefPubMedGoogle Scholar
  • 32 Lips KS, Yanko Ö, Kneffel M. et al. Small changes in bone structure of female α7 nicotinic acetylcholine receptor knockout mice. BMC Musculoskelet Disord 2015; 16 (01) 5
    CrossrefPubMedGoogle Scholar
  • 33 Kim JH, Kim N. Regulation of NFATc1 in osteoclast differentiation. J Bone Metab 2014; 21 (04) 233-241
    CrossrefPubMedGoogle Scholar
  • 34 Wu LZ, Duan DM, Liu YF, Ge X, Zhou ZF, Wang XJ. Nicotine favors osteoclastogenesis in human periodontal ligament cells co-cultured with CD4(+) T cells by upregulating IL−1β. Int J Mol Med 2013; 31 (04) 938-942
    CrossrefPubMedGoogle Scholar
  • 35 Rothem DE, Rothem L, Dahan A, Eliakim R, Soudry M. Nicotinic modulation of gene expression in osteoblast cells, MG−63. Bone 2011; 48 (04) 903-909
    CrossrefPubMedGoogle Scholar
  • 36 Asagiri M, Takayanagi H. The molecular understanding of osteoclast differentiation. Bone 2007; 40 (02) 251-264
    CrossrefPubMedGoogle Scholar
  • 37 Wang L, Chen K, He J. et al. Cytochalasin Z11 inhibits RANKL-induced osteoclastogenesis via suppressing NFATc1 activation. RSC Advances 2019; 9 (66) 38438-38446
    CrossrefPubMedGoogle Scholar