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Planta Med 2017; 83(10): 870-876
DOI: 10.1055/s-0043-102948
Formulation and Delivery Systems of Natural Products
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Bromelain Loading and Release from a Hydrogel Formulated Using Alginate and Arabic Gum

Janaína Artem Ataide
1   Postgraduate Program in Medical Sciences, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Brazil
,
Letícia Caramori Cefali
2   Postgraduate Program in Biosciences and Technology of Bioactive Products, Institute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil
,
Marcia de Araujo Rebelo
3   Laboratory of Biomaterials & Nanotechnology, Universidade de Sorocaba (UNISO), Sorocaba, Brazil
,
Lívia Genovez Spir
4   Faculty of Pharmaceutical Sciences, State University of Campinas (UNICAMP), Campinas, Brazil
,
Elias Basile Tambourgi
5   Chemical Engineering Systems Department, School of Chemical Engineering, State University of Campinas (UNICAMP), Campinas, Brazil
,
Angela Faustino Jozala
6   Department of Technological and Environmental Processes, Universidade de Sorocaba (UNISO), Sorocaba, Brazil
,
Marco Vinícius Chaud
3   Laboratory of Biomaterials & Nanotechnology, Universidade de Sorocaba (UNISO), Sorocaba, Brazil
,
Edgar Silveira
1   Postgraduate Program in Medical Sciences, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Brazil
7   Genetics and Biochemistry Institute, Uberlandia Federal University (UFU), Uberlandia, Brazil
,
Xiaochen Gu
8   College of Pharmacy, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada
,
Priscila Gava Mazzola
4   Faculty of Pharmaceutical Sciences, State University of Campinas (UNICAMP), Campinas, Brazil
› Institutsangaben
Weitere Informationen

Publikationsverlauf

received 19. Oktober 2016
revised 12. Januar 2017

accepted 26. Januar 2017

Publikationsdatum:
14. Februar 2017 (online)

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Abstract

An ideal wound dressing ensures a moist environment around the wound area and absorbs exudates from the wound surface. Topical application of bromelain to incised wounds has been shown to reprogram the wound microenvironment to promote effective tissue repair. Combining the characteristics of hydrogels and bromelain is therefore of great interest. Herein, we describe the development of a hydrogel, formulated using alginate and Arabic gum, for bromelain loading and release. The hydrogel formulation was evaluated using response surface methodology, considering the pH value and the concentration of alginate and Arabic gum. Bromelain loading and release were evaluated based on passive diffusion. Differential scanning calorimetry and Fourier transform infrared spectroscopy were performed to confirm bromelain immobilization in the hydrogel. The final hydrogel formulation had a swelling ratio of 227 % and incorporated 19 % of bromelain from a bromelain solution. Bromelain immobilization in the hydrogel was the result of hydrogen bond formation and was optimal at 4 °C after 4 h of contact. This evidence suggests that bromelain entrapment into a hydrogel is a promising strategy for the development of wound dressings that support the debridement of burns and wounds.

Key words

bromelain - hydrogel - alginate - Arabic gum - formulation development - Ananas comosus L. - Bromeliaceae
 

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