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Transfusionsmedizin 2017; 7(02): 100-103
DOI: 10.1055/s-0043-104895
Praxistipp
Georg Thieme Verlag KG Stuttgart · New York

Nachweis und Bedeutung von Kidd-Antikörpern im Festphasensystem (Capture-R® Ready-Screen®, Immucor)

Detection and Significance of Kidd-Antibodies in Solid Phase Red Cell Adherence System (Capture-R® Ready-Screen®, Immucor)
Sabine Flommersfeld
Universitätsklinikum Gießen und Marburg, Standort Marburg, Zentrum für Transfusionsmedizin und Hämotherapie, Marburg
,
Birgit Ertl
Universitätsklinikum Gießen und Marburg, Standort Marburg, Zentrum für Transfusionsmedizin und Hämotherapie, Marburg
› Author Affiliations
Further Information

Publication History

Publication Date:
30 May 2017 (online)

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Zusammenfassung

Der Nachweis von Kidd-Antikörpern gilt unter Immunhämatologen als besonders anspruchsvoll, da der Antikörper als transfusionsmedizinisch relevant eingestuft wird, aber nicht konsistent in allen Untersuchungsmethoden und oft nur passager nachweisbar ist. In Laboratorien, in denen der Capture-R® Ready-Screen®-Test zur Antikörpersuche eingeführt wurde, werden Kidd-Antikörper signifikant häufiger nachgewiesen als in der Röhrchen- oder Gelkartenzentrifugationsmethode im indirekten Antihumanglobulintest. Ob diese Antikörper, die nun vermehrt im Festphasensystem zu finden sind, klinisch auch eine Relevanz haben, ist insbesondere auch nach einer Publikation von Kay et al. in der Diskussion. Im Folgenden soll die Bedeutung des Nachweises der Kidd-Antikörper und deren klinischer Wertigkeit anhand der Literatur und den eigenen Daten dargestellt werden.

Abstract

The recognition of Kidd antibodies is known to be challenging. Oftentimes the antibody is only temporary identifiable, not visible consistently in all methods but relevant in transfusion matters, for it is known to be involved in acute and delayed transfusion reactions even with fatal outcome. In laboratories in which the Capture-R® Ready-Screen® was introduced for routine antibody screen, Kidd antibodies are found significantly more often in comparison to column agglutination or tube method. The clinical significance of Kidd antibodies that are found only in solid phase red cell adherence assay is yet unknown. Kay et al. assume after analysis of their own data that hemolytic transfusion reactions are caused by these antibodies but can be prevented if the antibody is found early enough by a sensitive method.

Schlüsselwörter

Festphase - Gelzentrifugation - Kidd-Antikörper - hämolytische Transfusionsreaktion

Key words

solid phase - column agglutination - Kidd antibodies - hemolytic transfusion reaction
 

  • Literatur

  • 1 Allen FH, Diamond LK, Niedziela B. A new blood-group antigen. Nature 1951; 167: 482
    PubMedGoogle Scholar
  • 2 Reid ME, Lomas-Francis C. eds. The Blood Group Antigen FactsBook. 2nd ed.. New York: Elsevier; 2007
    Google Scholar
  • 3 Hamilton JR. Kidd blood group system: a review. Immunohematology 2015; 31: 29-35
    PubMedGoogle Scholar
  • 4 Yates J, Howell P, Overfield J. et al. IgG anti-Jka/Jkb antibodies are unlikely to fix complement. Transf Med 1998; 8: 133-140
    CrossrefPubMedGoogle Scholar
  • 5 Reverberi R. The persistence of red cell alloantibodies. Blood Transfus 2008; 6: 225-234
    PubMedGoogle Scholar
  • 6 Ostendorf N, Niefhoff D, Cassens U. et al. Automated serological compatibility testing using a solid-phase test and standard laboratory equipment. Vox Sang 2001; 80: 225-229
    CrossrefPubMedGoogle Scholar
  • 7 Weisbach V, Kohnhäuser T, Zimmermann R. et al. Comparison of the performance of microtube column systems and solid-phase systems and the tube low-ionic-strength solution additive indirect antiglobulin test in the detection of red cell antibodies. Transfus Med 2006; 16: 276-284
    CrossrefPubMedGoogle Scholar
  • 8 Yamada C, Serrano-Rahman L, Vasovic LV. et al. Antibody identification using both automated solid-phase red cell adherence assay and a tube polyethylene glycol antiglobulin method. Transfusion 2008; 48: 1693-1698
    CrossrefPubMedGoogle Scholar
  • 9 Haywood JR, Moulds MK, Bryant BJ. Determination of optimal method for antibody identification in a reference laboratory. Immunohematology 2011; 27: 146-151
    PubMedGoogle Scholar
  • 10 Schmidt A, Bendix BJ, Eapen EK. et al. Single center comparison of gel microcolumn and solid-phase methods for antibody screening. Immunohematology 2013; 29: 101-104
    PubMedGoogle Scholar
  • 11 Kay B, Poisson JL, Tuma CW. et al. Anti-Jka that are detected by solid-phase red blood cell adherence but missed by gel testing can cause hemolytic transfusion reactions. Transfusion 2016; 56: 2973-2979
    CrossrefPubMedGoogle Scholar
  • 12 Bolton-Maggs PH. Annual shot report. Im Internet: https://www.shotuk.org/wp-content/uploads/SHOT-2015-Annual-Report-Web-Edition-Final&%236;-bookmarked-1.pdf Stand: 21.03.2017
    PubMedGoogle Scholar
  • 13 Paul-Ehrlich-Institut. Hämovigilanzbericht des Paul-Ehrlich-Instituts 2013/2014. Langen. Im Internet: http://www.pei.de/haemovigilanzbericht Stand: 21.03.2017
    PubMedGoogle Scholar
  • 14 Winters JL, Richa EM, Bryant SC. et al. Polyethylene glycol antiglobulin tube versus gel microcolumn: influence on the incidence of delayed hemolytic transfusion reactions and delayed serologic transfusion reactions. Transfusion 2010; 50: 1444-1452
    CrossrefPubMedGoogle Scholar