Abstract
Introduction In this retrospective study partial genotyping of human papilloma viruses (HPV) using
the Abbott RealTime HighRisk HPV Test (RealTime) was compared with simple HPV detection
(Qiagen Hybrid Capture 2 Test; hc2) for recurrence prediction at the first follow-up
examination after conization of cervical intraepithelial neoplasia (CIN).
Methods 144 women who had undergone conization for CIN between January 2007 and December
2013 were included. HPV status was determined preoperatively and at first follow-up
using hc2 in 103 women and RealTime in 41 women. Recurrent or persistent CIN was assumed
when CIN2+ was confirmed histologically or on comparable cytology findings.
Results Of the 144 women with complete data 12 (8.3%) had a recurrence after conization.
HPV persistence at follow-up correlated significantly with recurrence (hc2: p = 0.003;
RealTime: p = 0.003) and both sensitivity and specificity were high (hc2 = 100 and
78.4% respectively; RealTime = 75.0 and 83.9%). Whereas isolated HPV testing had a
relatively low positive predictive value for recurrence (hc2 16%; RealTime 54.5%),
this rose to 80% with HPV 16 detection at follow-up.
Conclusion At follow-up after conization of CIN the combination of high risk HPV detection and
partial genotyping of HPV 16 constitutes excellent diagnostic criteria for recurrence/persistence
of CIN.
Key words
conization - human papilloma virus - cervical intraepithelial neoplasia - HPV-genotyping
