Drug Res (Stuttg) 2018; 68(02): 113-120
DOI: 10.1055/s-0043-119073
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Curcumin Protects Mitochondria and Cardiomyocytes from Oxidative Damage and Apoptosis Induced by Hemiscorpius Lepturus Venom

Parvaneh Naserzadeh
1   Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
,
Sara Nekhoee Mehr
1   Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
,
Zeinab Sadabadi
1   Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
,
Enayatollah Seydi
2   Department of Occupational Health Engineering, Research Center for Health, Safety and Environment (RCHSE), Alborz University of Medical Sciences, Karaj, Iran
,
Ahmad Salimi
3   Department of Pharmacology and Toxicology, School of Pharmacy, Ardabil University of Medical Science, Ardabil, Iran
,
Jalal Pourahmad
1   Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
› Institutsangaben
Weitere Informationen

Publikationsverlauf

received 08. Juli 2017

accepted 22. August 2017

Publikationsdatum:
10. Oktober 2017 (online)

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Abstract

The main aim of the current study was to determine cardio-toxicity mechanisms of H. lepturus and protective effect of curcumin against this toxin in rats, using isolated heart mitochondria and cardiomyocytes. Our findings indicated that H. lepturus venom caused significantly ((P<0.05) cytotoxicity and caspase 3 activation in cardiomyocytes and mitochondrial dysfunction including increased mitochondrial ROS level, swelling in the mitochondria, decline in the mitochondria membrane potential (MMP), decrease in the cytochrome-c oxidase activity (complex IV), decrease ATP level and finally mitochondrial outer membrane (MOM) rupture in isolated mitochondria. Our results showed that the administration of curcumin efficiently decreased (P<0.05) cytotoxicity and caspase 3 activation, ROS formation, MMP collapse, mitochondrial swelling and mitochondrial outer membrane (MOM) rupture. Our findings suggest H. lepturus venom cusses a disruptive effect on mitochondrial respiratory chain, especially on complex II, and IV that predispose cardiomyocytes to ATP depletion and death signaling that could be protected with administration of curcumin.