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Drug Res (Stuttg) 2018; 68(04): 205-212
DOI: 10.1055/s-0043-119461
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Evaluation of Paclitaxel Nanocrystals In Vitro and In Vivo

Wanqing Li
1   School of Basic Medicine, Beijing University of Chinese Medicine, Beijing, P.R.China
,
Zhiguo Li
2   State Key Laboratory of Toxicology and Medical Countermeasures Department of Pharmaceutics, Beijing Institute of Pharmacology and Toxicology, Beijing, P.R.China
,
Lisha Wei
2   State Key Laboratory of Toxicology and Medical Countermeasures Department of Pharmaceutics, Beijing Institute of Pharmacology and Toxicology, Beijing, P.R.China
,
Aiping Zheng
2   State Key Laboratory of Toxicology and Medical Countermeasures Department of Pharmaceutics, Beijing Institute of Pharmacology and Toxicology, Beijing, P.R.China
› Author Affiliations
Further Information

Publication History

received 05 July 2017

accepted 07 September 2017

Publication Date:
30 November 2017 (online)

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Abstract

We created a novel paclitaxel (PTX) nanoparticle drug delivery system and compared this to acommercial injection preparation to evaluate the antitumor effects for both formulations in vivo and in vitro.PTXnanocrystals were 194.9 nm with potential of −29.6 mV. Cytotoxicity tests indicated that both formulations had similar effects and cytotoxicity was dose- and time-dependent.Pharmacodynamics indicated that the drug concentration at the tumor was greater with PTX nanocrystals compared to commercial injection (P<0.01) and that drug accumulated more and for a longer duration. In vivo antitumor evaluation indicated significant antitumor effects and low toxicity of PTX nanocrystals. Moreover, bioimaging indicated that the PTX retention time in MCF-7-bearing mice was longer, especially at the tumor site, and this high drug concentration was maintained for a long time.Overall, PTX nanocrystalsare feasible and superior to traditional injection formulation chemotherapy.

Key words

paclitaxel - nanocrystals - MCF-7 - pharmacodynamics - bioimaging
 

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