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Planta Medica International Open 2017; 4(03): e93-e103
DOI: 10.1055/s-0043-121151
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Identification of Bioactive Compounds in Polar and Nonpolar Extracts of Araujia sericifera

Martina Palomino-Schätzlein
1   Laboratorio de Bioquímica Estructural, Centro de Investigación Príncipe Felipe, Valencia, Spain
,
Mary Cecilia Montaño
2   Centro Ecología Química Agrícola, Instituto Agroforestal Mediterráneo, Universidad Politécnica de Valencia, Valencia, Spain
,
Pablo V. Escrig
3   Instituto de Tecnología Química (UPV-CSIC), Valencia, Spain
,
Herminio Boira
4   Instituto Agroforestal Mediterráneo, Universidad Politécnica de Valencia, Valencia, Spain
,
Avelino Corma
3   Instituto de Tecnología Química (UPV-CSIC), Valencia, Spain
,
Antonio Pineda-Lucena
1   Laboratorio de Bioquímica Estructural, Centro de Investigación Príncipe Felipe, Valencia, Spain
,
Jaime Primo
2   Centro Ecología Química Agrícola, Instituto Agroforestal Mediterráneo, Universidad Politécnica de Valencia, Valencia, Spain
,
Nuria Cabedo
2   Centro Ecología Química Agrícola, Instituto Agroforestal Mediterráneo, Universidad Politécnica de Valencia, Valencia, Spain
› Author Affiliations
Further Information

Publication History

received 06 April 2017
revised 07 June 2017

accepted 25 September 2017

Publication Date:
09 November 2017 (online)

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Abstract

Araujia sericifera is a native perennial, climbing laticiferous shrub from South America that is currently naturalized in many other countries. Previous data describe promising properties for A. sericifera, but no systematic study of its bioactive compounds and possible medicinal applications has been conducted to date. In the present study, aerial parts of A. sericifera (leaves, stems, and fruits) were explored by combining GC-MS and NMR spectroscopy analysis for both nonpolar (hexane) and polar (methanol) extracts. The hexanic extracts contained high amounts of pentacyclic triterpenes including two new metabolites, 3-tigloyl germanicol (18) and 3-tigloyl lupeol (19). The methanolic extracts revealed the presence of luteolin-7-glucoside (24), trigonelline (22), and conduritol F (23) as the main constituents. A multivariate study of a meaningful number of extracts allowed us to determine the distribution of compounds inside the plant. A cytotoxic evaluation in vitro showed that both leaf and fruit hexanic extracts presented a moderate activity against human breast carcinoma cell lines (MDA-MB-453 and MCF-7) and human colon carcinoma cell line (HCT-116) by the MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] assay.

Key words

Araujia sericifera - Asclepiadaceae - metabolite profile - cancer cell lines - GC-MS - NMR spectroscopy

Supporting Information

    Details on extraction and properties of the extracts as Supporting information is available online at http://www.thieme-connect.de/products
  • Supporting Information
 

  • References

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