Solvent Extraction and GC-MS Analysis of Sesame Seeds for Determination of Bioactive Antioxidant Fatty Acid/Fatty Oil Components

Abstract

The seed kernels of Sesamum indicum L. (family: Pedaliaceae) were extracted with ethanol and yield of components determined by Gas Chromatography/Mass Spectrometry (GC/MS). The free radical scavenging activities of ethanolic extract against1, 1-Diphenyl-2-picrylhydrazyl (DPPH) were determined by UV spectrophotometer at 517 nm. Phytochemical screening revealed the presence of numerous bioactive compounds including steroids, phenolic, terpenoids, fatty acids and different types of ester compounds. The ethanolic extract was purified and analyzed by GC MS.The prevailing compounds found in ethanolic extract were Carvacrol (0.04%),Sesamol (0.11%), 4-Allyl-2-methoxy-phenol(0.04%),Palmitic acid (1.08%), cis-9-Hexadecenal (85.40%), Lineoleoyl chloride (0.52%), Palmitic acid β-monoglyceride (0.40%), Dihydro-aplotaxene (0.61%), Oleoyl chloride (1.11%), (+)-Sesamin (4.73%), 1,3-Benzodioxole, 5-[4-(1,3-benzodioxol-5-yloxy)tetrahydro-1 H,3 H-furo [3,4-c]furan-1-yl], [1 S-(1,3,4,6α.), (2.01%)], 6-Nitrocholest-5-en-3-yl acetate (0.22%), Ergost-5-en-3β-ol (2.35%) and 24-Propylidenecholesterol (0.16%). The presence ofsaturated and unsaturated fatty acids in ethanolicextract justifies the use of this plant to treat many ailments in folk and traditional medicine. Ethanolic extract have shown significant antioxidant activity(IC₅₀120.38±2.8 µg/ml). The presence of phenolic (Sesamol), lignin (Sesamin) compounds and unsaturated fatty acids are reported as possible contributor for antioxidantactivity of seed extract.

Key words

Sesamum indicum - Lignan - Sesamol - total antioxidant capacity - DPPH assay and GC/MS

• References

• 1 Martin JH, Leonard WH. Miscellaneous industries crops. In: Principles of Field Crop Production. New York: Macmillan; 1967: 922-924
• 2 Ogasawara T, Chiba K, Tada M. In Bajaj YPS. ed. Medicinal and Aromatic Plants. Volume 10 Springer; 1988. ISBN 3-540-62727-8
• 3 Abou-Gharbia HA, Shehata AA, Shahidi F. Effect of processing on oxidative stability and lipid classes of sesame oil. Food Research International 2000; 33: 331-340
• 4 Kato MJ, Chu A, Davin LB. et al. Biosynthesis of antioxidant lignans in in Sesamum indicum seeds. Phytochemistry 1998; 47: 583-591
• 5 Shasmitha R. Health benefits of Sesamum Indicum: A short Review. Asian Journal of Pharmaceutical and Clinical Research 2015; 8: 1-3
• 6 Sachan NK, Arif M, Zaman K. et al. Anti-inflammatory, analgesic and antioxidant potential of the stem bark of Spondias mangifera Wild. Archives of Biological Sciences 2011; 63: 413-419
• 7 Khandelwal KR. Practical pharmacognosy techniques & experiments XIII Ed. Pune: Nirali Prakashan; 2004: 149-156
• 8 Qadir A, Singh SP, Akhtar J. et al. Phytochemical and GC-MS analysis of Saudi Arabian Ajwa variety of date seed oil and extracts obtained by the slow pyrolysis method. Oriental Pharmacy and Experimental Medicine 2017; 17: 81-87
• 9 Adams RP. Identification of essential oil components by gas chromatography/mass spectrometry. 2007. 4th ed. Carol Stream, IL: Allured Publ. Corp.;
• 10 Arif M, Fareed S, Hussain MS. Estimation of antioxidant activity of microwave assisted extraction of total phenolics and flavonoids contents of the fruit Spondias mangifera. Asian Journal of Traditional Medicines 2011; 6: 146-155
• 11 Wynn JP, Kendrick A, Ratledge C. Sesamol as an inhibitor of growth and lipid metabolism in Mucor circinelloides via its action on malic enzyme. Lipids 1997; 32: 605-610
• 12 Jacklin A, Ratledge C, Welham K. et al. The sesame seed oil constituent, sesamol, induces growth arrest and apoptosis of cancer and cardiovascular cells. Annals of the New York Academy of Sciences 2003; 1010: 374-380
• 13 Lee CC, Chen PR, Lin S. et al. Sesamin induces nitric oxide and decreases endothelin-1 production in HUVECs: Possible implications for its antihypertensive effect. Journal of Hypertension 2004; 12: 2329-2338
• 14 Ghafoorunissa S, Hemalatha M, Rao VV. Sesame lignans enhance antioxidant activity of vitamin E in lipid peroxidation systems. Molecular and Cellular Biochemistry 2004; 262: 195-202
• 15 Arif M, Fareed S, Hussain T. et al. Adaptogenic activity of lanostane triterpenoid isolated from Carissa carandas fruit against physically and chemically challenged experimental mice. Journal of Pharmacy and Bioallied Sciences 2013; 5: 216-220