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CC BY 4.0 · Eur J Dent 2023; 17(04): 1215-1220
DOI: 10.1055/s-0043-1761191
Original Article

Differential Expression Profile of Salivary oncomiRNAs among Smokeless Tobacco Users

Authors

  • Natheer H. AL-Rawi 

    1   Department of Oral & Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates
    2   Sharjah Institute of Medical Research, University of Sharjah, Sharjah, United Arab Emirates

  • Zuha Rizvi

    1   Department of Oral & Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates
    2   Sharjah Institute of Medical Research, University of Sharjah, Sharjah, United Arab Emirates

  • Sarra Mkadmi

    1   Department of Oral & Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates

  • Rawan Abu Kou

    1   Department of Oral & Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates

  • Neibal Elmabrouk

    1   Department of Oral & Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates

  • Mohammad S. Alrashdan

    1   Department of Oral & Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates

  • Aghila Rani Koippallil Gopalakrishnan

    2   Sharjah Institute of Medical Research, University of Sharjah, Sharjah, United Arab Emirates

Funding This work was supported by the University of Sharjah [grant number 2101100139].
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Abstract

Objective The aim of this study was to evaluate the expression of selected salivary oncomiRNAs among smokeless tobacco users and nonsmokers.

Materials and Methods Twenty-five subjects with chronic smokeless tobacco habit (> 1 year) and 25 nonsmokers were selected for this study. MicroRNA was extracted from saliva samples using the miRNeasy Kit (Qiagen, Hilden, Germany). The forward primers used in the reactions include hsa-miR-21-5p, hsa-miR-146a-3p, hsa-miR-155-3p, and hsa-miR-199a-3p. Relative expression of miRNAs was calculated using the 2-ΔΔCt method. Fold change is calculated by raising 2 to the power of the negative ΔΔCT value.

Statistical Analysis Statistical analysis was carried out using GraphPad Prism 5 software. A p-value less than 0.05 was considered statistically significant.

Results The four tested miRNAs were found overexpressed in saliva of subjects with smokeless tobacco habit when compared with saliva from nontobacco users. miR-21 expression was 3.74 ± 2.26 folds higher among subjects with smokeless tobacco habit compared to nontobacco users (p < 0.01). The expression for miR-146a (5.56 ± 8.3 folds; p < 0.05), miR-155 (8.06 ± 23.4 folds; p < 0.0001) and miR-199a (14.39 ± 30.3 folds; p < 0.05) was significantly higher among subjects with smokeless tobacco habit.

Conclusion Smokeless tobacco leads to salivary overexpression of the miRs 21, 146a, 155, and 199a. Monitoring the levels of these four oncomiRs may provide insight about the future development of oral squamous cell carcinoma, especially in patients with smokeless tobacco habits.

Keywords

smokeless tobacco - miR-21 - miR-146a - miR-155 - miR-199a - oral cancer

Supplementary Material



Publikationsverlauf

Artikel online veröffentlicht:
22. Februar 2023

© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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