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CC BY 4.0 · Eur J Dent 2024; 18(01): 131-137
DOI: 10.1055/s-0043-1761194
Original Article

BDNF and Krox20 as Indicators of Platelet-rich Plasma-Induced Nerve Regeneration in a Neuropathic Orofacial Pain Model

Saka Winias 
1   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Andari Sarasati 
2   Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Satutya Wicaksono 
2   Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Nurina Febriyanti Ayuningtyas 
1   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Diah Savitri Ernawati 
1   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Desiana Radithia 
1   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
› Author Affiliations Financial Support This research is financially supported by the Ministry of Research, Technology & Higher Education of Indonesia [grant numbers 27/E1/KPT/2020].
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Abstract

Objective Various growth factors contained in PRP can increase angiogenesis and cell proliferation, which plays an essential role in the process of neuroregeneration and peripheral nerve injury recovery. This study analyzed PRP effects in the neuro-regeneration of axonotmesis through brain-derived neurotrophic factor (BDNF) and Krox20 expressions.

Materials and Methods Freeze-dried allogeneic platelet-rich plasma (PRP) were prepared from allogeneic sources. Forty-two Rattus norvegicus were divided into three groups: negative control group, positive control group (crushing infraorbital nerve) and treatment group (crushing infraorbital nerve without PRP injection). Each group was observed for fourteen and twenty-one days after injury. Infraorbital nerve tissue is isolated for indirect immunohistochemistry examination with BDNF and Krox20 antibodies. Data analysis was performed using One-Way ANOVA and Mann-Whitney tests with significant value as p < 0.05.

Results The PRP group showed BDNF expression significantly higher than control positive groups, both observation days (p = 0.00). A higher Korx20 expression showed by the PRP group after 21 days than in the control positive groups (p = 0.002).

Conclusion PRP can potentially improve neuroregeneration of axonotmesis through increased BDNF and Krox20 expression on the twenty-one days after injury.

Keywords

peripheral nerve injury - neuro regeneration - BDNF - PRP - Krox20

Authors' Contribution

SW, AS, SW2, NFA, DSE, DR designed the study, performed the data collection, data analysis and interpretation, wrote the manuscript and reviewed the manuscript. All authors declare that they contributed to the critical review of intellectual content and approval of the final version to be published.




Publication History

Article published online:
13 June 2023

© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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