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CC BY-NC-ND 4.0 · Ann Natl Acad Med Sci 2023; 59(02): 097-103
DOI: 10.1055/s-0043-1768136
Original Article

Identification of Bacterial Isolates and Their Antimicrobial Susceptibility Pattern from Wound/Pus Sample in a Tertiary Care Hospital, Gwalior, India

Kamini Rajput 
1   Department of Life Science, ITM University, Gwalior, Madhya Pradesh, India
,
Sonia Johri
1   Department of Life Science, ITM University, Gwalior, Madhya Pradesh, India
,
Anju Goyal
2   Department of Microbiology, Birla Institute of Medical Research Hospital, Gwalior, Madhya Pradesh, India
› Institutsangaben Funding None.
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Abstract

Objective The goal of this investigation was to look at the frequency and dispersal of bacteria isolated from pus/wound, as well as their susceptibility patterns.

Materials and Methods A study was conducted on 175 patients who provided pus and/or wound discharge samples in different wards (outpatient department or inpatient department). MacConkey agar and blood agar plates were immediately inoculated with samples and incubated at 37°C for 24 hours. The Gram stain and biochemical tests were used to identify all isolates after incubation. Kirby–Bauer's disc diffusion method was used to perform sensitivity tests on Mueller–Hinton agar plates.

Results This study covered 175 patients, with a bacterial isolation rate of 102 (58.28%). Males outnumbered females in the samples (M:F = 1.8:1), with a median age of 45 years as majority were in the age group of 40 to 60 years which was 41 (40.20%). Total 90.1% samples showed monomicrobial infection, whereas 9.8% showed polymicrobial infection, and total 112 bacterial strains were isolated.

Conclusion Escherichia coli was the most prevalent isolate in present investigation, followed by Pseudomonas aeruginosa. Chloramphenicol is the only antibiotic which is effective for both gram-negative bacilli and gram-positive cocci. This report's susceptibility statistic may be worth considering for developing empiric treatment regimens for pyogenic infections.

Keywords

pus sample - wound infection - antimicrobial susceptibility - pyogenic - multidrug resistant - GNB - GPC

Ethical Approval

The study was approved by the head of the institute where study was done.




Publikationsverlauf

Artikel online veröffentlicht:
16. Juni 2023

© 2023. National Academy of Medical Sciences (India). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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