Download PDF
CC BY-NC-ND 4.0 · Ann Natl Acad Med Sci 2023; 59(02): 097-103
DOI: 10.1055/s-0043-1768136
Original Article

Identification of Bacterial Isolates and Their Antimicrobial Susceptibility Pattern from Wound/Pus Sample in a Tertiary Care Hospital, Gwalior, India

Kamini Rajput 
1   Department of Life Science, ITM University, Gwalior, Madhya Pradesh, India
,
Sonia Johri
1   Department of Life Science, ITM University, Gwalior, Madhya Pradesh, India
,
Anju Goyal
2   Department of Microbiology, Birla Institute of Medical Research Hospital, Gwalior, Madhya Pradesh, India
› Author Affiliations Funding None.
› Further Information
Also available at
   Permissions and Reprints

Abstract

Objective The goal of this investigation was to look at the frequency and dispersal of bacteria isolated from pus/wound, as well as their susceptibility patterns.

Materials and Methods A study was conducted on 175 patients who provided pus and/or wound discharge samples in different wards (outpatient department or inpatient department). MacConkey agar and blood agar plates were immediately inoculated with samples and incubated at 37°C for 24 hours. The Gram stain and biochemical tests were used to identify all isolates after incubation. Kirby–Bauer's disc diffusion method was used to perform sensitivity tests on Mueller–Hinton agar plates.

Results This study covered 175 patients, with a bacterial isolation rate of 102 (58.28%). Males outnumbered females in the samples (M:F = 1.8:1), with a median age of 45 years as majority were in the age group of 40 to 60 years which was 41 (40.20%). Total 90.1% samples showed monomicrobial infection, whereas 9.8% showed polymicrobial infection, and total 112 bacterial strains were isolated.

Conclusion Escherichia coli was the most prevalent isolate in present investigation, followed by Pseudomonas aeruginosa. Chloramphenicol is the only antibiotic which is effective for both gram-negative bacilli and gram-positive cocci. This report's susceptibility statistic may be worth considering for developing empiric treatment regimens for pyogenic infections.

Keywords

pus sample - wound infection - antimicrobial susceptibility - pyogenic - multidrug resistant - GNB - GPC

Ethical Approval

The study was approved by the head of the institute where study was done.




Publication History

Article published online:
16 June 2023

© 2023. National Academy of Medical Sciences (India). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India

 

  • References

  • 1 Aftab S, Tarik M, Siddique A, Yusuf A. Clinical and microbiological aspect of wound infection: a review update. Bangladesh Journal of Infectious Diseases. 2014; 1 (02) 32-36
    CrossrefSearch in Google Scholar
  • 2 Mukherjee S, Mishra S, Tiwary S. Microbial profile and antibiogram of pus isolate in a tertiary care hospital of Western Odisha. J Evol Med Dent Sci 2020; 9 (16) 1325-1330
    CrossrefSearch in Google Scholar
  • 3 Ennab R, AI-Momani W, AI-Titi R, Elayan A. Antibiotic profile of pathogenic bacteria isolated from postsurgical site infections in public hospitals in Northern Jordan. Infect Drug Resist 2022; 15: 359-366
    CrossrefPubMedSearch in Google Scholar
  • 4 Sharma V, Parihar G, Sharma V, Sharma H. A study of various isolates from pus sample with their antibiogram from Jln hospital, Ajmer. IOSR J Dent Med Sci 2015; 14 (10) 64-68
    Search in Google Scholar
  • 5 Insan NG, Payal N. Post-operative wound infection: bacteriology and antibiotic sensitivity pattern. Int J Curr Res Rev 2013; 05 (13) 74-79
    Search in Google Scholar
  • 6 Kumwenda P, Adukwu EC, Tabe ES. et al. Prevalence, distribution and antimicrobial susceptibility pattern of bacterial isolates from a tertiary hospital in Malawi. BMC Infect Dis 2021; 21 (34) 1-10
    PubMedSearch in Google Scholar
  • 7 Muluye D, Wondimeneh Y, Ferede G. et al. Bacterial isolates and their antibiotic susceptibility patterns among patients with pus and/or wound discharge at Gondar University Hospital. BMC Res Notes 2014; 7 (619) 1-5
    PubMedSearch in Google Scholar
  • 8 Trojan R, Razdan L, Singh N. Antibiotic susceptibility patterns of bacterial isolates from pus samples in a tertiary care hospital care hospital of Punjab, India. Int J Microbiol 2016; 16: 1-4
    CrossrefPubMedSearch in Google Scholar
  • 9 Wadekar MD, Sathish JV, Jayashree, Pooja C. Bacteriological profile of pus samples and their antibiotic susceptibility pattern. Indian J Microbiol Res 2020; 7 (01) 43-47
    CrossrefSearch in Google Scholar
  • 10 Misha G, Chelkeba L, Melaku T. Bacterial profile and antimicrobial susceptibility patterns of isolates among patients diagnosed with surgical site infection at a tertiary teaching hospital in Ethiopia: a prospective cohort study. Ann Clin Microbiol Antimicrob 2021; 20 (33) 1-10
    PubMedSearch in Google Scholar
  • 11 Sudhaharan S, Kanne P, Chavali P, Vemu L. Aerobic bacteriological profile and antimicrobial susceptibility pattern of pus isolates from tertiary care hospital in India. J Infect Dev Ctries 2018; 12 (10) 842-848
    CrossrefPubMedSearch in Google Scholar
  • 12 Duggal S, Khatri PK, Parihar RS. et al. Antibiogram of various bacterial isolates from pus samples in a tertiary care centre in Rajasthan. Int J Sci Res 2015; 4 (05) 1580-1584
    Search in Google Scholar
  • 13 Singh A, Verma V, Singh R. Antibiotic sensitivity pattern of pathogens isolated from pus culture- A tertiary care hospital based study. Journal of Nepalgunj Medical College. 2019; 17 (02) 70-74
    CrossrefSearch in Google Scholar
  • 14 Rameshkannan S, Nileshraj G, Rameshprabu S, Mangaiarkkarasi A, Meher R. Pattern of pathogens and their sensitivity isolated from pus culture reports in a tertiary care hospital, Puducherry. Indian J Basic Appl Med Res 2014; 4 (01) 243-248
    Search in Google Scholar
  • 15 Hailu D, Mekonnen D, Derbie A. et al. Pathogenic bacteria profile and antimicrobial susceptibility patterns of ear infection at Bahir Dar Regional Health Research Laboratory Center, Ethiopia. Springerplus 2016; 16 (05) 466-471
    CrossrefPubMedSearch in Google Scholar
  • 16 Mondal SN, Islam A, Zerin T. Characterization and Antibiotic Susceptibility of bacteria isolated from clinical specimens in Rajshahi, Bangladesh. Int J Health Sci Res 2022; 12 (02) 16-25
    CrossrefSearch in Google Scholar
  • 17 Huda N, Yusuf A, Sultana H, Hossain M, Andalib S. Antimicrobial sensitivity pattern of bacteria isolated from pus sample collected from a private diagnostic laboratory in Rangpur district of Bangladesh. Bangladesh J Infect Dis 2021; 8 (02) 64-70
    CrossrefSearch in Google Scholar
  • 18 Moussa AA, Abdi AA, Awale MA, Garba B. Occurrence and phenotypic characterization of multidrug-resistant bacterial pathogen isolated from patients in a public hospital in Mogadishu, Somalia. Infect Drug Resist 2021; 14: 825-832
    CrossrefPubMedSearch in Google Scholar
  • 19 Mengesha RE, Kasa BG, Saravanan M, Berhe DF, Wasihun AG. Aerobic bacteria in post-surgical wound infections and pattern of their antimicrobial susceptibility in Ayder teaching and referral hospital, Mekelle, Ethiopia. BMC Res Notes 2014; 7: 575-580
    CrossrefPubMedSearch in Google Scholar
  • 20 Omoyibo EE, Oladele AO, Ibrahim MH, Adekunle OT. Antibiotic susceptibility of wound swab isolates in a tertiary hospital in southwest Nigeria. Ann Afr Med 2018; 17 (03) 110-116
    CrossrefPubMedSearch in Google Scholar
  • 21 Khanam RA, Islam R, Sharif A, Rezina P, Sharmin I, Yusuf A. Bacteriological profiles of pus with antimicrobial sensitivity pattern at a teaching hospital in Dhaka City. Bangladesh J Infection Dis 2018; 5 (01) 10-14
    CrossrefSearch in Google Scholar
  • 22 The Clinical and Laboratory Standards Institute. CLSI Supplement M100 – Performance Standards for Antimicrobial Susceptibility Testing. 32nd ed.. Malvern, Pennsylvania, United States: The Clinical and Laboratory Standards Institute; 2022
    Search in Google Scholar
  • 23 Rai S, Yadav UN, Pant ND. et al. Bacteriological profile and antimicrobial susceptibility patterns of bacteria isolated from pus/wound swab samples from children attending a tertiary care hospital in Kathmandu, Nepal. Int J Microbiol 2017; 17: 1-5
    CrossrefPubMedSearch in Google Scholar
  • 24 Batra S, Veena B, Agarwal S, Sharma R. Bacteriological profile and antimicrobial susceptibility pattern of pus culture isolates from a tertiary care hospital, SMS Medical College Jaipur. Eur J Mol Clin Med 2020; 07 (11) 7502-7508
    Search in Google Scholar
  • 25 Bankar N, Wankhade A, Brahmane RB, Hathiwala R, Chandi DH. Bacteriological profile of Pus/Wound swab and antimicrobial susceptibility of Staphylococcus aureus isolated from pus and wound swab of indoor patients of tertiary care hospital, Durg, Chhattisgarh, India. International Journal of Innovative Research in Medical Science. 2018; 03 (04) 1-5
    Search in Google Scholar
  • 26 Davies J, Davies D. Origins and evolution of antibiotic resistance. Microbiol Mol Biol Rev 2010; 74 (03) 417-433
    CrossrefPubMedSearch in Google Scholar