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CC BY-NC-ND 4.0 · Ann Natl Acad Med Sci 2023; 59(02): 104-108
DOI: 10.1055/s-0043-1768461
Original Article

Detection of Antibiotic Resistance and Biofilm-Producing Ability of Staphylococcus Species in Clinical Isolates

Shila Kumari Singh
1   Department of Microbiology, Faculty of Sciences, Assam Downtown University, Guwahati, Assam, India
,
Minakshi Bhattacharjee
2   Department of Biotechnology, Faculty of Sciences, Assam Downtown University, Guwahati, Assam, India
,
Balagopalan Unni
3   Department of Research, Assam Downtown University, Guwahati, Assam, India
,
Rajpal Singh Kashyap
4   Department of Research, Central India Institute of Medical Science, Nagpur, Maharashtra, India
› Author Affiliations Funding None.
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Abstract

Background Staphylococci are responsible for life-threatening infections in hospitals and community. Their ability to produce multiple virulence factors and antibiotic resistance is an important reason of high mortality in staphylococcal infections. Biofilm production by these organisms makes it difficult to treat. Most of the treating antibiotics are failing and making it a matter of concern.

Aims This study aims to detect the increased antibiotic resistance in biofilm-producing Staphylococcus and to compare the performance of three potential methods of detection.

Methods A total of 81 isolates of staphylococci including coagulase negative staphylococci (CoNs), methicillin resistant S. aureus (MRSA), and methicillin sensitive S. aureus (MSSA) are included in this study. After the identification, an antibiotic sensitivity test was performed. Biofilm detection was done by three different methods: Congo red agar method, tube adherence method, and microtiter plate method.

Result Out of the 81 samples, 37 CoNs, 17 MRSA, and 27 MSSA were identified. Out of them we got 43 (53%) biofilm producers by Congo red agar method, 40 (49%) by tube adherence method, and 52 (64%) producers by tissue culture plate/microtiter plate method. Most of the biofilm producers showed multiple drug resistance.

Conclusion We found out that the microtiter plate method is sensitive and reliable as compared with the other two methods. Antibiotic resistance was found to be very common in biofilm producers. This was due to the resistance developed as a result of the matrix that does not let the antibiotic bind with the organisms. This can make the treatment of Staphylococcus very difficult in the future as the rate of drug resistance is faster as compared with newly emerging antibiotics.

Keywords

biofilm - antibiotic resistance - MRSA - MSSA - CoNs


Publication History

Article published online:
07 July 2023

© 2023. National Academy of Medical Sciences (India). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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