Effect of ABCA1-R219K Polymorphism in Serum Lipid Parameters in Patients under Statin Therapy Visiting Tertiary Cardiac Center of Nepal

Abstract

Introduction ATP-binding cassette transporter A1 (ABCA1) encoded by ABCA1 gene is one of the important protein involved in lipid metabolism. The effect of statin therapy on dyslipidemia varies among individuals and it may be due to different genetic polymorphism. The R219K polymorphism of ABCA1 gene is found to have a significant role in the response of statin.

Objective This study was designed to evaluate the effect of R219K polymorphism in lipid-lowering action of statin in patients with dyslipidemia.

Material and Methods This study was conducted in 88 patients. Blood samples were taken from patients before and at the end of 3 months of statin use and were analyzed for lipid profile. Whole blood was analyzed for R219K Polymorphism using polymerase chain reaction-restriction fragment length polymorphism.

Results R219K polymorphism was associated with significant percentage reduction of serum triglyceride/high-density lipoprotein (TG/HDL) ratio and total cholesterol/high-density lipoprotein (TC/HDL) ratio in atorvastatin users. However, there was no significant association of polymorphism with change in serum TC, HDL-C, LDL-C, TG, and very low-density lipoprotein (VLDL). Among KK genotype individuals, value of TG, VLDL, TG/HDL, and TC/HDL were significantly lower than in RR genotypes. Also, TG/HDL and TC/HDL were significantly lower in RK genotype than in RR. Treatment of dyslipidemia with statin was found to be comparatively better in patients having the genotypes KK and RK.

Conclusion Our study demonstrated association of R219K polymorphism with the significant reduction of TG/HDL and TC/HDL and particularly the KK genotype was associated with significant improvement of lipid parameters following atorvastatin treatment.

Keywords

R219K polymorphism - PCR-RFLP - lipid profile - single-nucleotide polymorphisms - dyslipidemia - atorvastatin - statin therapy

Data Availability

All the data used and/or analyzed in this study can make available from the corresponding author on request.

Patient Consent

The informed consent was obtained from all the individual patients.

Ethical Approval

This study was approved by the Institutional Review committee (IRC), Institute of Medicine (IOM), Kathmandu, Nepal (IRC reference number: 320(6–11-E) 2/074/075).

Consent for Publication

I, Binod Kumar Yadav, corresponding author of this manuscript give full consent for publication on behalf of all co-authors.

Authors' Contribution

S.G. performed laboratory work, statistical analysis, and manuscript writing. BKY helped in designing the study, analysis of the data, and writing of the manuscript and proof reading. J.S. contributed to laboratory work standardization, data analysis, and proof reading. S.S. and D.S. supported in laboratory work. V.K.S., E.T.T., M.R., and A.B. conceived the study and participated in the design and coordination of the study. K.D.M., C.M.P., and V.P. read and approved the final manuscript.

Publikationsverlauf

Eingereicht: 22. September 2022

Angenommen: 26. Februar 2023

Artikel online veröffentlicht:
03. Juli 2023

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