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CC BY-NC-ND 4.0 · J Lab Physicians 2023; 15(04): 573-577
DOI: 10.1055/s-0043-1770067
Original Article

In Vitro Susceptibility of Burkholderia pseudomallei Isolates to Cefiderocol and Ceftazidime/Avibactam from Odisha, India

Jayanti Jena
1   Department of Microbiology, All India Institute of Medical Sciences (AIIMS), Bhubaneswar, Odisha, India
,
Bijayini Behera 
1   Department of Microbiology, All India Institute of Medical Sciences (AIIMS), Bhubaneswar, Odisha, India
,
Gayatree Nayak
1   Department of Microbiology, All India Institute of Medical Sciences (AIIMS), Bhubaneswar, Odisha, India
,
Srujana Mohanty 
1   Department of Microbiology, All India Institute of Medical Sciences (AIIMS), Bhubaneswar, Odisha, India
,
Ashoka Mahapatra
1   Department of Microbiology, All India Institute of Medical Sciences (AIIMS), Bhubaneswar, Odisha, India
,
Prashanth Purushotham
1   Department of Microbiology, All India Institute of Medical Sciences (AIIMS), Bhubaneswar, Odisha, India
,
Anjuna Radhakrishnan 
1   Department of Microbiology, All India Institute of Medical Sciences (AIIMS), Bhubaneswar, Odisha, India
,
Manaswiny Tripathy
1   Department of Microbiology, All India Institute of Medical Sciences (AIIMS), Bhubaneswar, Odisha, India
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Abstract

Introduction and Objectives The availability of a limited arsenal of antibacterial agents effective against Burkholderia pseudomallei, the causative agent of melioidosis, together with sporadic reports of emergence of resistance necessitates an evaluation of in vitro activity of new antimicrobials against clinical B. pseudomallei isolates. Cefiderocol (CFDC), a novel siderophore cephalosporin, and ceftazidime-avibactam (CZA), a new β lactam combination agent, have shown promising results for the treatment of difficult-to-treat Gram-negative bacilli infections with limited treatment options. This study was conducted to determine the in vitro activity of CFDC and CZA against a contemporary collection of 60 B. pseudomallei clinical isolates.

Materials and Methods Minimum inhibitory concentrations (MIC) of CFDC and CZA were determined by broth microdilution and E-test, respectively. The performance of disk diffusion was also evaluated for CFDC.

Results All B. pseudomallei isolates were susceptible to CFDC and CZA with MIC range of 0.125 to 2 mg/L and 0.19 to 1 mg/L, respectively. Zone diameters for CFDC ranged from 31 to 40 mm.

Conclusion CFDC and CZA exhibited excellent in vitro activity against 60 B. pseudomallei isolates. Further pharmacokinetic-pharmacodynamics studies and clinical trials are needed to prove the clinical efficacy of CFDC and CZA in the treatment of melioidosis.

Keywords

Burkholderia pseudomallei - Cefiderocol - Ceftazidime-avibactam


Publication History

Received: 20 January 2023

Accepted: 19 April 2023

Article published online:
13 June 2023

© 2023. The Indian Association of Laboratory Physicians. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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