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J Pediatr Infect Dis 2023; 18(05): 250-255
DOI: 10.1055/s-0043-1770989
Original Article

Antibiofilm Activity of Different Washing Solutions against Biofilms in Cystic Fibrosis Patients' Nasal Cavities

Gökhan Tüzemen 
1   Department of Otorhinolaryngology-Head and Neck Surgery, Bursa City Hospital, Mınıstry of Health, Bursa, Türkiye
,
Nazmiye Ülkü Tüzemen 
2   Department of Medical Microbiology, Faculty of Medicine, Bursa Uludag University, Bursa, Türkiye
,
Perihan Erkan Alkan 
3   Department of Medical Microbiology, Faculty of Medicine, Bandırma Onyedi Eylül University, Bandırma, Balıkesir, Türkiye
,
Cüneyt Özakın 
2   Department of Medical Microbiology, Faculty of Medicine, Bursa Uludag University, Bursa, Türkiye
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Abstract

Objective Bacterial biofilm formation is a multistep process involving bacterial adhesion to inorganic or mucosal surfaces. We aimed to identify Staphylococcus aureus and Pseudomonas aeruginosa strains colonizing the respiratory tracts of cystic fibrosis (CF) patients and to gauge the antibiofilm potential of streptomycin and ozone solutions against them.

Methods Bacteria were obtained from CF patients' sputum samples processed in our microbiology laboratory over 1 year (2021–2022). A total of 26 nonduplicate strains (13 S. aureus and 13 P. aeruginosa) were included in this study.

Results Both ozone and streptomycin solutions showed significant inhibitory activity. However, when faced with mature biofilm, the streptomycin solution had a significantly more substantial impact than the ozone solution. Furthermore, the ozone solution had no inhibitory effect on mature P. aeruginosa biofilm.

Conclusion Ozone and streptomycin solutions might be used as nasal irrigation to eliminate the biofilms in patients with CF in acute respiratory infections. However, our in vitro observations would need to be confirmed in vivo. In chronic inflammation, ozone solution cannot degrade the mature biofilm of P. aeruginosa, whereas streptomycin solution can degrade such biofilms. This result is promising in lessening the biofilms associated with these bacteria that colonize patients with CF.

Keywords

antibiofilm - S. aureus - P. aeruginosa - ozone - streptomycin


Publication History

Received: 09 February 2023

Accepted: 31 May 2023

Article published online:
24 July 2023

© 2023. Thieme. All rights reserved.

Georg Thieme Verlag KG
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