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CC BY-NC-ND 4.0 · Rev Bras Ortop (Sao Paulo) 2023; 58(05): e798-e807
DOI: 10.1055/s-0043-1771490
Artigo Original
Trauma

Characterization of the Masquelet Induced Membrane Technique in a Murine Segmental Bone Defect Model

Article in several languages: português | English
João Antonio Matheus Guimarães 
1   Coordenador de pós-graduação, Instituto Nacional de Traumatologia e Ortopedia, Rio de Janeiro, RJ, Brasil
,
Breno Jorge Braga Scorza 
1   Coordenador de pós-graduação, Instituto Nacional de Traumatologia e Ortopedia, Rio de Janeiro, RJ, Brasil
,
Jamila Alessandra Perini Machado 
1   Coordenador de pós-graduação, Instituto Nacional de Traumatologia e Ortopedia, Rio de Janeiro, RJ, Brasil
3   Pesquisadora, Laboratório de Pesquisa de Ciências Farmacêuticas, Universidade do Estado do Rio de Janeiro (UERJ), Rio de Janeiro, Brasil
,
Amanda dos Santos Cavalcanti 
1   Coordenador de pós-graduação, Instituto Nacional de Traumatologia e Ortopedia, Rio de Janeiro, RJ, Brasil
,
Maria Eugênia Leite Duarte 
1   Coordenador de pós-graduação, Instituto Nacional de Traumatologia e Ortopedia, Rio de Janeiro, RJ, Brasil
2   Cirurgião ortopédico, Instituto D'Or de Ensino e Pesquisa, IDOR, Rio de Janeiro, RJ, Brasil
› Author Affiliations Financial Support The authors declare that they received no financial support from public, commercial or non-profit sources.
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Abstract

Objective To reproduce in an animal model the surgical technique of Masquelet used in the treatment of critical bone defects and to analyze the characteristics of the membrane formed around the bone cement.

Methods A 10mm critical defect was created in the femoral shaft of 21 Sprague-Dawley rats. After resection of the central portion of the diaphysis, the defect was stabilized with a Kirschner wire introduced through the medullary canal and with the interposition of a bone cement spacer. After 2, 4, and 6 weeks of the surgical procedure, the animals were euthanized and evaluated on radiographs of the posterior limb regarding the size of the defect, alignment and stability of the osteosynthesis. The membranes formed around the spacer were subjected to histological analysis to assess thickness, connective tissue maturation and vascular density.

Results Over time, the membranes initially made up of loose connective tissue were replaced by membranes represented by dense connective tissue, rich in thick collagen fibers. At six weeks, membrane thickness was greater (565 ± 208μm) than at four (186.9 ± 70.21μm, p = 0.0002) and two weeks (252.2 ± 55.1μm, p = 0.001). All membranes from the initial time showed foci of osteogenic differentiation that progressively reduced over time.

Conclusion In addition to the structural and protective function of the membrane, its intrinsic biological characteristics can actively contribute to bone regeneration. The biological activity attributed by the presence of foci of osteogenesis confers to the membrane the potential of osteoinduction that favors the local conditions for the integration of the bone graft.

Keywords

bone and bones - masquelet technique - models, animal - membrane induced - bone regeneration

Work developed at the National Institute of Traumatology and Orthopedics, Rio de Janeiro, RJ, Brazil.




Publication History

Received: 23 September 2022

Accepted: 16 December 2022

Article published online:
30 October 2023

© 2023. Sociedade Brasileira de Ortopedia e Traumatologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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