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CC BY 4.0 · Pharmaceutical Fronts 2023; 05(04): e288-e296
DOI: 10.1055/s-0043-1777285
Original Article

Solvents Influence 1H NMR Chemical Shifts and Complete 1H and 13C NMR Spectral Assignments for Florfenicol

Wan-Ting Ai
1   Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Huzhou, People's Republic of China
,
Wei-Ke Su
1   Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Huzhou, People's Republic of China
2   Zhejiang Governor Triangle Biomedical Industrial Technology Research Park, Zhejiang University of Technology, Huzhou, People's Republic of China
,
Feng Su
1   Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Huzhou, People's Republic of China
2   Zhejiang Governor Triangle Biomedical Industrial Technology Research Park, Zhejiang University of Technology, Huzhou, People's Republic of China
› Institutsangaben Funding The study is supported by the Zhejiang Science and Technology Plan Project (Grant No. 2021C03161), the Zhejiang Provincial Key R&D Project (Grant No. 2020C03006 & 2019-ZJ-JS-03), and the Natural Science Foundation of Zhejiang Province, China (Grant No. LQ18B050003).
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Abstract

Florfenicol (FFC) is an important and widely used veterinary drug, and its structure has been characterized by nuclear magnetic resonance (NMR) spectroscopy. The study aimed to investigate the influences of solvent type, solvent concentration, and temperature on the chemical shifts of the 1H NMR of FFC. The results showed that different types of solvents significantly affected the chemical shifts, especially the chemical shifts of 2-H, 3-H, 5-H, and the active protons. When DMSO-d 6 is used as the solvent, there is no significant difference in the chemical shifts of FFC with a concentration ranging from 20 to 250 mmol/L; however, as the temperature increases, the chemical shifts of the active protons move to a higher field. Besides, the NMR spectroscopic data and structural analysis of FFC were refined by 1H, 13C, distortionless enhancement by polarization transfer-135 (DEPT-135), 1H–1H correlation spectroscopy (1H–1H COSY), phase-sensitive gradient heteronuclear singular quantum correlation (gHSQC), and heteronuclear multiple bond correlation (gHMBC) NMR spectroscopy using DMSO-d 6 as a solvent. The study will help with qualitative and quantitative analysis of FFC in the future.

Keywords

florfenicol - nuclear magnetic resonance - solvents - solvent concentrations - chemical shifts


Publikationsverlauf

Eingereicht: 29. Juni 2023

Angenommen: 31. Oktober 2023

Artikel online veröffentlicht:
01. Dezember 2023

© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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