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Exp Clin Endocrinol Diabetes 2020; 128(02): 77-81
DOI: 10.1055/s-0044-100376
Article
© Georg Thieme Verlag KG Stuttgart · New York

The Variation of Disulfides in the Progression of Type 2 Diabetes Mellitus

Merve Ergin
1   Department of Biochemistry, 25 Aralik State Hospital, Gaziantep, Turkey
,
Cevdet Aydin
2   Department of Endocrinology and Metabolism, Yildirim Beyazit University, Faculty of Medicine, Ankara, Turkey
,
Emine Feyza Yurt
3   Department of Biochemistry, Yildirim Beyazit University, Faculty of Medicine, Ankara, Turkey
,
Bekir Cakir
2   Department of Endocrinology and Metabolism, Yildirim Beyazit University, Faculty of Medicine, Ankara, Turkey
,
Ozcan Erel
3   Department of Biochemistry, Yildirim Beyazit University, Faculty of Medicine, Ankara, Turkey
› Institutsangaben
Weitere Informationen

Publikationsverlauf

received 05. August 2017
revised 25. Dezember 2017

accepted 08. Januar 2018

Publikationsdatum:
29. Januar 2018 (online)

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Abstract

Aim The purpose of this study was to examine thiol-disulfide balance in patients with type 2 diabetes mellitus.

Methods This study included 32 subjects with known type 2 diabetes mellitus without complications, 30 patients with type 2 diabetes mellitus with complications, 28 newly diagnosed patients with type 2 diabetes mellitus, and 45 healthy individuals. Thiol-disulfide profile tests were quantified in all groups.

Results Compared to the control group, patients in each of the diabetic groups had significantly lower native and total thiol levels, higher disulfide levels, and higher disulfide/native thiol and disulfide/total thiol ratios (p<0.05 for all). Disulfide levels were significantly lower in the newly diagnosed group than in other diabetic groups (p<0.05). There were significant associations between glycemic parameters and thiol-disulfide tests (p<0.05).

Conclusions A disequilibrium between thiol-disulfide pairs occurs in patients with type 2 diabetes mellitus, and a gradual increase to disulfide levels may contribute to the disease’s severity. Deteriorated thiol-disulfide homeostasis may be relevant to the pathophysiology of type 2 diabetes mellitus.

Key words

disulfide - oxidative stress - thiol-disulfide homeostasis - type 2 diabetes mellitus
 

  • References

  • 1 Adeshara KA, Diwan AG, Jagtap TR. et al. Relationship between plasma glycation with membrane modification, oxidative stress and expression of glucose trasporter-1 in type 2 diabetes patients with vascular complications. J Diabetes Complications 2017; 31: 439-448
    CrossrefPubMedSuche in Google Scholar
  • 2 Annadurai T, Vasanthakumar A, Geraldine P. et al. Variations in erythrocyte antioxidant levels and lipid peroxidation status and in serum lipid profile parameters in relation to blood haemoglobin A1c values in individuals with type 2 diabetes mellitus. Diabetes Res Clin Pract 2014; 105: 58-69
    CrossrefPubMedSuche in Google Scholar
  • 3 Zephy D, Ahmad J. Type 2 diabetes mellitus: Role of melatonin and oxidative stress. Diabetes Metab Syndr 2015; 9: 127-131
    CrossrefPubMedSuche in Google Scholar
  • 4 Butkowski EG, Jelinek HF. Hyperglycaemia, oxidative stress and inflammatory markers. Redox Rep. 2017; 22: 257-264
    CrossrefPubMedSuche in Google Scholar
  • 5 Pisoschi AM, Pop A. The role of antioxidants in the chemistry of oxidative stress: A review. Eur J Med Chem 2015; 97: 55-74
    CrossrefPubMedSuche in Google Scholar
  • 6 Poljsak B, Šuput D, Milisav I. Achieving the balance between ROS and antioxidants: when to use the synthetic antioxidants. Oxid Med Cell Longev 2013; 2013: 956792
    PubMedSuche in Google Scholar
  • 7 Matteucci E, Giampietro O. Thiol signalling network with an eye to diabetes. Molecules 2010; 15: 8890-8903
    CrossrefPubMedSuche in Google Scholar
  • 8 Turell L, Radi R, Alvarez B. The thiol pool in human plasma: the central contribution of albumin to redox processes. Free Radic Biol Med. 2013; 65: 244-253
    CrossrefPubMedSuche in Google Scholar
  • 9 Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem 2014; 47: 326-332
    Suche in Google Scholar
  • 10 Kolgelier S, Ergin M, Saltuk Demir L. et al. Impaired thiol-disulfide balance in acute brucellosis. Jpn J Infect Dis 2017; 70: 258-262
    Suche in Google Scholar
  • 11 Ozler S, Oztas E, Caglar AT. et al. Thiol/disulfide homeostasis in predicting adverse perinatal outcomes at 24–28 weeks of pregnancy in gestational diabetes. J Matern Fetal Neonatal Med 2016; 29: 3699-3704
    CrossrefPubMedSuche in Google Scholar
  • 12 Ergin M, Caliskanturk M, Senat A. et al. Disulfide stress in carbon monoxide poisoning. Clin Biochem 2016; 49: 1243-1247
    CrossrefPubMedSuche in Google Scholar
  • 13 Ates I, Kaplan M, Inan B. et al. How does thiol/disulfide homeostasis change in prediabetic patients?. Diabetes Res Clin Pract 2015; 110: 166-171
    CrossrefPubMedSuche in Google Scholar
  • 14 Ergin M, Cendek BD, Neselioglu S. et al. Dynamic thiol-disulfide homeostasis in hyperemesis gravidarum. J Perinatol. 2015; 35: 788-792
    CrossrefPubMedSuche in Google Scholar
  • 15 Grindel A, Guggenberger B, Eichberger L. et al. Oxidative stress, DNA damage and DNA repair in female patients with diabetes mellitus type 2. PLoS One 2016; 11: e0162082
    CrossrefPubMedSuche in Google Scholar
  • 16 Arif M, Islam MR, Waise TM. et al. DNA damage and plasma antioxidant indices in Bangladeshi type 2 diabetic patients. Diabetes Metab 2010; 36: 51-57
    CrossrefPubMedSuche in Google Scholar
  • 17 Duman BS, Ozturk M, Yilmazeri S. et al. Thiols, malonaldehyde and total antioxidant status in the Turkish patients with type 2 diabetes mellitus. Tohoku J Exp Med 2003; 201: 147-155
    CrossrefPubMedSuche in Google Scholar
  • 18 Piwowar A, Knapik-Kordecka M, Warwas M. Markers of oxidative protein damage in plasma and urine of type 2 diabetic patients. Br J Biomed Sci 2009; 66: 194-199
    PubMedSuche in Google Scholar
  • 19 Rani AJ, Mythili SV. Study on total antioxidant status in relation to oxidative stress in type 2 diabetes mellitus. J Clin Diagn Res 2014; 8: 108-110
    CrossrefPubMedSuche in Google Scholar
  • 20 Allen EM, Mieyal JJ. Protein-thiol oxidation and cell death: regulatory role of glutaredoxins. Antioxid Redox Signal. 2012; 17: 1748-1763
    CrossrefPubMedSuche in Google Scholar
  • 21 Lu SC. Glutathione synthesis. Biochim Biophys Acta. 2013; 1830: 3143-3153
    CrossrefPubMedSuche in Google Scholar
  • 22 Spanidis Y, Mpesios A, Stagos D. et al. Assessment of the redox status in patients with metabolic syndrome and type 2 diabetes reveals great variations. Exp Ther Med 2016; 11: 895-903
    CrossrefPubMedSuche in Google Scholar
  • 23 Sekhar RV, McKay SV, Patel SG. et al. Glutathione synthesis is diminished in patients with uncontrolled diabetes and restored by dietary supplementation with cysteine and glycine. Diabetes care 2011; 34: 162-167
    CrossrefPubMedSuche in Google Scholar
  • 24 Ates I, Kaplan M, Yuksel M. et al. Determination of thiol/disulphide homeostasis in type 1 diabetes mellitus and the factors associated with thiol oxidation. Endocrine 2016; 51: 47-51
    CrossrefPubMedSuche in Google Scholar
  • 25 Manna P, Jain SK. L-cysteine and hydrogen sulfide increase PIP3 and AMPK/PPARgamma expression and decrease ROS and vascular inflammation markers in high glucose treated human U937 monocytes. J Cell Biochem 2013; 114: 2334-2345
    CrossrefPubMedSuche in Google Scholar
  • 26 Carter RN, Morton NM. Cysteine and hydrogen sulphide in the regulation of metabolism: insights from genetics and pharmacology. J Pathol 2016; 238: 321-332
    CrossrefPubMedSuche in Google Scholar
  • 27 Hsu CC, Yen HF, Yin MC. et al. Five cysteine-containing compounds delay diabetic deterioration in Balb/cA mice. J Nutr 2004; 134: 3245-3249
    CrossrefPubMedSuche in Google Scholar
  • 28 Achari AE, Jain SK. L-Cysteine supplementation increases adiponectin synthesis and secretion, and GLUT4 and glucose utilization by upregulating disulfide bond A-like protein expression mediated by MCP-1 inhibition in 3T3-L1 adipocytes exposed to high glucose. Mol Cell Biochem 2016; 414: 105-113
    CrossrefPubMedSuche in Google Scholar
  • 29 Ju Y, Untereiner A, Wu L. et al. H2S-induced S-sulfhydration of pyruvate carboxylase contributes to gluconeogenesis in liver cells. Biochim Biophys Acta. (BBA)-General Subjects 2015; 1850: 2293-2303
    CrossrefPubMedSuche in Google Scholar